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Mol Cell Biol, May 1998, p. 2876-2883, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Stepwise Recruitment of Components of the Preinitiation Complex by Upstream Activators In Vivo

Song He and Steven Jay Weintraub*

Departments of Internal Medicine and Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, Missouri 63110

Received 14 August 1997/Returned for modification 16 October 1997/Accepted 12 February 1998

Recently, it was found that if either the TATA binding protein or RNA polymerase II holoenzyme is artificially tethered to a promoter, transcription is activated. This finding provided presumptive evidence that upstream activating proteins function by recruiting components of the preinitiation complex (PIC) to the promoter. To date, however, there have been no studies demonstrating that upstream factors actually recruit components of the PIC to the promoter in vivo. Therefore, we have studied the mechanism of action of two disparate transactivating domains. We present a series of in vivo functional assays that demonstrate that each of these proteins targets different components of the PIC for recruitment. We show that, by targeting different components of the PIC for recruitment, these activating domains can cooperate with each other to activate transcription synergistically and that, even within one protein, two different activating subdomains can activate transcription synergistically by cooperating to recruit different components of the PIC. Finally, considering our work together with previous studies, we propose that certain transcription factors both recruit components of the PIC and facilitate steps in transcriptional activation that occur subsequent to recruitment.

* Corresponding author. Mailing address: Departments of Internal Medicine and Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Ave., Campus Box 8052, Saint Louis, MO 63110. Phone: (314) 362-8964. Fax: (314) 362-8963. E-mail: weintrau{at}im.wustl.edu.

Mol Cell Biol, May 1998, p. 2876-2883, Vol. 18, No. 5
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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