This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kiefer, F.
Right arrow Articles by Pawson, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kiefer, F.
Right arrow Articles by Pawson, T.

 Previous Article  |  Next Article 

Mol Cell Biol, July 1998, p. 4209-4220, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Syk Protein Tyrosine Kinase Is Essential for Fcgamma Receptor Signaling in Macrophages and Neutrophils

Friedemann Kiefer,1 John Brumell,1 2 Nadia Al-Alawi,1 Sylvain Latour,3 Alec Cheng,1 André Veillette,3 Sergio Grinstein,2 and Tony Pawson1 4 *

Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5,1 Hospital for Sick Children, Toronto, Ontario M5G 1X8,2 McGill Cancer Centre, McGill University, Montreal, Quebec H3G 1Y6,3 and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A8,4 Canada

Received 24 February 1998/Accepted 20 April 1998

The cytoplasmic protein tyrosine kinase Syk has two amino-terminal SH2 domains that engage phosphorylated immunoreceptor tyrosine-based activation motifs in the signaling subunits of immunoreceptors. Syk, in conjunction with Src family kinases, has been implicated in immunoreceptor signaling in both lymphoid and myeloid cells. We have investigated the role of Syk in Fcgamma receptor (Fcgamma R)-dependent and -independent responses in bone marrow-derived macrophages and neutrophils by using mouse radiation chimeras reconstituted with fetal liver cells from Syk-/- embryos. Chimeric mice developed an abdominal hemorrhage starting 2 to 3 months after transplantation that was ultimately lethal. Syk-deficient neutrophils derived from the bone marrow were incapable of generating reactive oxygen intermediates in response to Fcgamma R engagement but responded normally to tetradecanoyl phorbol acetate stimulation. Syk-deficient macrophages were defective in phagocytosis induced by Fcgamma R but showed normal phagocytosis in response to complement. The tyrosine phosphorylation of multiple cellular polypeptides, including the Fcgamma R gamma  chain, as well as Erk2 activation, was compromised in Syk-/- macrophages after Fcgamma R stimulation. In contrast, the induction of nitric oxide synthase in macrophages stimulated with lipopolysaccharide and gamma interferon was not dependent on Syk. Surprisingly, Syk-deficient macrophages were impaired in the ability to survive or proliferate on plastic petri dishes. Taken together, these results suggest that Syk has specific physiological roles in signaling from Fcgamma Rs in neutrophils and macrophages and raise the possibility that in vivo, Syk is involved in signaling events other than those mediated by immunoreceptors.

* Corresponding author. Mailing address: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada. Phone: (416) 586-8262. Fax: (416) 586-8857. E-mail: Pawson{at}mshri.on.ca.

Mol Cell Biol, July 1998, p. 4209-4220, Vol. 18, No. 7
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Van Ziffle, J. A., Lowell, C. A. (2009). Neutrophil-specific deletion of Syk kinase results in reduced host defense to bacterial infection. Blood 114: 4871-4882 [Abstract] [Full Text]  
  • Schymeinsky, J., Gerstl, R., Mannigel, I., Niedung, K., Frommhold, D., Panthel, K., Heesemann, J., Sixt, M., Quast, T., Kolanus, W., Mocsai, A., Wienands, J., Sperandio, M., Walzog, B. (2009). A fundamental role of mAbp1 in neutrophils: impact on {beta}2 integrin-mediated phagocytosis and adhesion in vivo. Blood 114: 4209-4220 [Abstract] [Full Text]  
  • Zhu, S., Wurdak, H., Wang, Y., Galkin, A., Tao, H., Li, J., Lyssiotis, C. A., Yan, F., Tu, B. P., Miraglia, L., Walker, J., Sun, F., Orth, A., Schultz, P. G., Wu, X. (2009). A genomic screen identifies TYRO3 as a MITF regulator in melanoma. Proc. Natl. Acad. Sci. USA 106: 17025-17030 [Abstract] [Full Text]  
  • Kerrigan, A. M., Dennehy, K. M., Mourao-Sa, D., Faro-Trindade, I., Willment, J. A., Taylor, P. R., Eble, J. A., Reis e Sousa, C., Brown, G. D. (2009). CLEC-2 Is a Phagocytic Activation Receptor Expressed on Murine Peripheral Blood Neutrophils. J. Immunol. 182: 4150-4157 [Abstract] [Full Text]  
  • Jakus, Z., Simon, E., Frommhold, D., Sperandio, M., Mocsai, A. (2009). Critical role of phospholipase C{gamma}2 in integrin and Fc receptor-mediated neutrophil functions and the effector phase of autoimmune arthritis. JEM 206: 577-593 [Abstract] [Full Text]  
  • Tohyama, Y., Yamamura, H. (2009). Protein Tyrosine Kinase, Syk: A Key Player in Phagocytic Cells. J Biochem 145: 267-273 [Abstract] [Full Text]  
  • N'Diaye, E.-N., Branda, C. S., Branda, S. S., Nevarez, L., Colonna, M., Lowell, C., Hamerman, J. A., Seaman, W. E. (2009). TREM-2 (triggering receptor expressed on myeloid cells 2) is a phagocytic receptor for bacteria. JCB 184: 215-223 [Abstract] [Full Text]  
  • Haberzettl, P., Schins, R. P. F., Hohr, D., Wilhelmi, V., Borm, P. J. A., Albrecht, C. (2008). Impact of the Fc{gamma}II-receptor on quartz uptake and inflammatory response by alveolar macrophages. Am. J. Physiol. Lung Cell. Mol. Physiol. 294: L1137-L1148 [Abstract] [Full Text]  
  • Huang, Y.-W., Yan, M., Collins, R. F., DiCiccio, J. E., Grinstein, S., Trimble, W. S. (2008). Mammalian Septins Are Required for Phagosome Formation. Mol. Biol. Cell 19: 1717-1726 [Abstract] [Full Text]  
  • Lau, C., Wang, X., Song, L., North, M., Wiehler, S., Proud, D., Chow, C.-W. (2008). Syk Associates with Clathrin and Mediates Phosphatidylinositol 3-Kinase Activation during Human Rhinovirus Internalization. J. Immunol. 180: 870-880 [Abstract] [Full Text]  
  • Busetto, S., Trevisan, E., Decleva, E., Dri, P., Menegazzi, R. (2007). Chloride Movements in Human Neutrophils during Phagocytosis: Characterization and Relationship to Granule Release. J. Immunol. 179: 4110-4124 [Abstract] [Full Text]  
  • Popa-Nita, O., Rollet-Labelle, E., Thibault, N., Gilbert, C., Bourgoin, S. G., Naccache, P. H. (2007). Crystal-induced neutrophil activation. IX. Syk-dependent activation of class Ia phosphatidylinositol 3-kinase. J. Leukoc. Biol. 82: 763-773 [Abstract] [Full Text]  
  • Khandani, A., Eng, E., Jongstra-Bilen, J., Schreiber, A. D., Douda, D., Samavarchi-Tehrani, P., Harrison, R. E. (2007). Microtubules regulate PI-3K activity and recruitment to the phagocytic cup during Fc{gamma} receptor-mediated phagocytosis in nonelicited macrophages. J. Leukoc. Biol. 82: 417-428 [Abstract] [Full Text]  
  • Xu, J.-W., Morita, I., Ikeda, K., Miki, T., Yamori, Y. (2007). C-Reactive Protein Suppresses Insulin Signaling in Endothelial Cells: Role of Spleen Tyrosine Kinase. Mol. Endocrinol. 21: 564-573 [Abstract] [Full Text]  
  • Leeansyah, E., Wines, B. D., Crowe, S. M., Jaworowski, A. (2007). The Mechanism Underlying Defective Fc{gamma} Receptor-Mediated Phagocytosis by HIV-1-Infected Human Monocyte-Derived Macrophages. J. Immunol. 178: 1096-1104 [Abstract] [Full Text]  
  • Huang, Z.-Y., Barreda, D. R., Worth, R. G., Indik, Z. K., Kim, M.-K., Chien, P., Schreiber, A. D. (2006). Differential kinase requirements in human and mouse Fc-gamma receptor phagocytosis and endocytosis. J. Leukoc. Biol. 80: 1553-1562 [Abstract] [Full Text]  
  • Schymeinsky, J., Sindrilaru, A., Frommhold, D., Sperandio, M., Gerstl, R., Then, C., Mocsai, A., Scharffetter-Kochanek, K., Walzog, B. (2006). The Vav binding site of the non-receptor tyrosine kinase Syk at Tyr 348 is critical for beta2 integrin (CD11/CD18)-mediated neutrophil migration. Blood 108: 3919-3927 [Abstract] [Full Text]  
  • Trivedi, V., Zhang, S. C., Castoreno, A. B., Stockinger, W., Shieh, E. C., Vyas, J. M., Frickel, E.-M., Nohturfft, A. (2006). Immunoglobulin G signaling activates lysosome/phagosome docking. Proc. Natl. Acad. Sci. USA 103: 18226-18231 [Abstract] [Full Text]  
  • Wang, X., Lau, C., Wiehler, S., Pow, A., Mazzulli, T., Gutierrez, C., Proud, D., Chow, C.-W. (2006). Syk Is Downstream of Intercellular Adhesion Molecule-1 and Mediates Human Rhinovirus Activation of p38 MAPK in Airway Epithelial Cells. J. Immunol. 177: 6859-6870 [Abstract] [Full Text]  
  • Abtahian, F., Bezman, N., Clemens, R., Sebzda, E., Cheng, L., Shattil, S. J., Kahn, M. L., Koretzky, G. A. (2006). Evidence for the Requirement of ITAM Domains but Not SLP-76/Gads Interaction for Integrin Signaling in Hematopoietic Cells.. Mol. Cell. Biol. 26: 6936-6949 [Abstract] [Full Text]  
  • Shi, Y., Tohyama, Y., Kadono, T., He, J., Shahjahan Miah, S. M., Hazama, R., Tanaka, C., Tohyama, K., Yamamura, H. (2006). Protein-tyrosine kinase Syk is required for pathogen engulfment in complement-mediated phagocytosis. Blood 107: 4554-4562 [Abstract] [Full Text]  
  • Cougoule, C., Hoshino, S., Dart, A., Lim, J., Caron, E. (2006). Dissociation of Recruitment and Activation of the Small G-protein Rac during Fc{gamma} Receptor-mediated Phagocytosis. J. Biol. Chem. 281: 8756-8764 [Abstract] [Full Text]  
  • Underhill, D. M., Rossnagle, E., Lowell, C. A., Simmons, R. M. (2005). Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 106: 2543-2550 [Abstract] [Full Text]  
  • Bennouna, S., Denkers, E. Y. (2005). Microbial Antigen Triggers Rapid Mobilization of TNF-{alpha} to the Surface of Mouse Neutrophils Transforming Them into Inducers of High-Level Dendritic Cell TNF-{alpha} Production. J. Immunol. 174: 4845-4851 [Abstract] [Full Text]  
  • Scott, C. C., Dobson, W., Botelho, R. J., Coady-Osberg, N., Chavrier, P., Knecht, D. A., Heath, C., Stahl, P., Grinstein, S. (2005). Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis. JCB 169: 139-149 [Abstract] [Full Text]  
  • Ulanova, M., Puttagunta, L., Marcet-Palacios, M., Duszyk, M., Steinhoff, U., Duta, F., Kim, M.-K., Indik, Z. K., Schreiber, A. D., Befus, A. D. (2005). Syk tyrosine kinase participates in {beta}1-integrin signaling and inflammatory responses in airway epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 288: L497-L507 [Abstract] [Full Text]  
  • Clemens, R. A., Newbrough, S. A., Chung, E. Y., Gheith, S., Singer, A. L., Koretzky, G. A., Peterson, E. J. (2004). PRAM-1 Is Required for Optimal Integrin-Dependent Neutrophil Function. Mol. Cell. Biol. 24: 10923-10932 [Abstract] [Full Text]  
  • Herre, J., Marshall, A. S. J., Caron, E., Edwards, A. D., Williams, D. L., Schweighoffer, E., Tybulewicz, V., Sousa, C. R. e, Gordon, S., Brown, G. D. (2004). Dectin-1 uses novel mechanisms for yeast phagocytosis in macrophages. Blood 104: 4038-4045 [Abstract] [Full Text]  
  • Yamauchi, A., Kim, C., Li, S., Marchal, C. C., Towe, J., Atkinson, S. J., Dinauer, M. C. (2004). Rac2-Deficient Murine Macrophages Have Selective Defects in Superoxide Production and Phagocytosis of Opsonized Particles. J. Immunol. 173: 5971-5979 [Abstract] [Full Text]  
  • Ryckman, C., Gilbert, C., de Medicis, R., Lussier, A., Vandal, K., Tessier, P. A. (2004). Monosodium urate monohydrate crystals induce the release of the proinflammatory protein S100A8/A9 from neutrophils. J. Leukoc. Biol. 76: 433-440 [Abstract] [Full Text]  
  • Huang, Z.-Y., Hunter, S., Kim, M.-K., Chien, P., Worth, R. G., Indik, Z. K., Schreiber, A. D. (2004). The monocyte Fc{gamma} receptors Fc{gamma}RI/{gamma} and Fc{gamma}RIIA differ in their interaction with Syk and with Src-related tyrosine kinases. J. Leukoc. Biol. 76: 491-499 [Abstract] [Full Text]  
  • Albelda, S. M., Lau, K. C., Chien, P., Huang, Z.-Y., Arguiris, E., Bohen, A., Sun, J., Billet, J. A., Christofidou-Solomidou, M., Indik, Z. K., Schreiber, A. D. (2004). Role for Platelet-Endothelial Cell Adhesion Molecule-1 in Macrophage Fc{gamma} Receptor Function. Am. J. Respir. Cell Mol. Bio. 31: 246-255 [Abstract] [Full Text]  
  • Jakus, Z., Berton, G., Ligeti, E., Lowell, C. A., Mocsai, A. (2004). Responses of Neutrophils to Anti-Integrin Antibodies Depends on Costimulation through Low Affinity Fc{gamma}Rs: Full Activation Requires Both Integrin and Nonintegrin Signals. J. Immunol. 173: 2068-2077 [Abstract] [Full Text]  
  • Ratthe, C., Girard, D. (2004). Interleukin-15 enhances human neutrophil phagocytosis by a Syk-dependent mechanism: importance of the IL-15R{alpha} chain. J. Leukoc. Biol. 76: 162-168 [Abstract] [Full Text]  
  • Song, X., Tanaka, S., Cox, D., Lee, S. C. (2004). Fc{gamma} receptor signaling in primary human microglia: differential roles of PI-3K and Ras/ERK MAPK pathways in phagocytosis and chemokine induction. J. Leukoc. Biol. 75: 1147-1155 [Abstract] [Full Text]  
  • Hewins, P., Williams, J. M., Wakelam, M. J.O., Savage, C. O.S. (2004). Activation of Syk in Neutrophils by Antineutrophil Cytoplasm Antibodies Occurs via Fc{gamma} Receptors and CD18. J. Am. Soc. Nephrol. 15: 796-808 [Abstract] [Full Text]  
  • Nichols, K. E., Haines, K., Myung, P. S., Newbrough, S., Myers, E., Jumaa, H., Shedlock, D. J., Shen, H., Koretzky, G. A. (2004). Macrophage activation and Fc{gamma} receptor-mediated signaling do not require expression of the SLP-76 and SLP-65 adaptors. J. Leukoc. Biol. 75: 541-552 [Abstract] [Full Text]  
  • Bennouna, S., Bliss, S. K., Curiel, T. J., Denkers, E. Y. (2003). Cross-Talk in the Innate Immune System: Neutrophils Instruct Recruitment and Activation of Dendritic Cells during Microbial Infection. J. Immunol. 171: 6052-6058 [Abstract] [Full Text]  
  • Yamamoto, N., Takeshita, K., Shichijo, M., Kokubo, T., Sato, M., Nakashima, K., Ishimori, M., Nagai, H., Li, Y.-F., Yura, T., Bacon, K. B. (2003). The Orally Available Spleen Tyrosine Kinase Inhibitor 2-[7-(3,4-Dimethoxyphenyl)-imidazo[1,2-c]pyrimidin-5-ylamino]nicotinamide Dihydrochloride (BAY 61-3606) Blocks Antigen-Induced Airway Inflammation in Rodents. J. Pharmacol. Exp. Ther. 306: 1174-1181 [Abstract] [Full Text]  
  • Gu, H., Botelho, R. J., Yu, M., Grinstein, S., Neel, B. G. (2003). Critical role for scaffolding adapter Gab2 in Fc{gamma}R-mediated phagocytosis. JCB 161: 1151-1161 [Abstract] [Full Text]  
  • Santini, V., Scappini, B., Indik, Z. K., Gozzini, A., Rossi Ferrini, P., Schreiber, A. D. (2003). The carboxy-terminal region of the granulocyte colony-stimulating factor receptor transduces a phagocytic signal. Blood 101: 4615-4622 [Abstract] [Full Text]  
  • Gilbert, C., Levasseur, S., Desaulniers, P., Dusseault, A.-A., Thibault, N., Bourgoin, S. G., Naccache, P. H. (2003). Chemotactic Factor-Induced Recruitment and Activation of Tec Family Kinases in Human Neutrophils. II. Effects of LFM-A13, a Specific Btk Inhibitor. J. Immunol. 170: 5235-5243 [Abstract] [Full Text]  
  • Mocsai, A., Zhang, H., Jakus, Z., Kitaura, J., Kawakami, T., Lowell, C. A. (2003). G-protein-coupled receptor signaling in Syk-deficient neutrophils and mast cells. Blood 101: 4155-4163 [Abstract] [Full Text]  
  • Worth, R. G., Kim, M.-K., Kindzelskii, A. L., Petty, H. R., Schreiber, A. D. (2003). Signal sequence within Fcgamma RIIA controls calcium wave propagation patterns: Apparent role in phagolysosome fusion. Proc. Natl. Acad. Sci. USA 100: 4533-4538 [Abstract] [Full Text]  
  • Kwiatkowska, K., Frey, J., Sobota, A. (2003). Phosphorylation of Fc{gamma}RIIA is required for the receptor-induced actin rearrangement and capping: the role of membrane rafts. J. Cell Sci. 116: 537-550 [Abstract] [Full Text]  
  • Sedlik, C., Orbach, D., Veron, P., Schweighoffer, E., Colucci, F., Gamberale, R., Ioan-Facsinay, A., Verbeek, S., Ricciardi-Castagnoli, P., Bonnerot, C., Tybulewicz, V. L. J., Di Santo, J., Amigorena, S. (2003). A Critical Role for Syk Protein Tyrosine Kinase in Fc Receptor-Mediated Antigen Presentation and Induction of Dendritic Cell Maturation. J. Immunol. 170: 846-852 [Abstract] [Full Text]  
  • Wen, R., Jou, S.-T., Chen, Y., Hoffmeyer, A., Wang, D. (2002). Phospholipase C{gamma}2 Is Essential for Specific Functions of Fc{epsilon}R and Fc{gamma}R. J. Immunol. 169: 6743-6752 [Abstract] [Full Text]  
  • Strzelecka-Kiliszek, A., Kwiatkowska, K., Sobota, A. (2002). Lyn and Syk Kinases Are Sequentially Engaged in Phagocytosis Mediated by Fc{gamma}R. J. Immunol. 169: 6787-6794 [Abstract] [Full Text]  
  • Coppolino, M. G., Dierckman, R., Loijens, J., Collins, R. F., Pouladi, M., Jongstra-Bilen, J., Schreiber, A. D., Trimble, W. S., Anderson, R., Grinstein, S. (2002). Inhibition of Phosphatidylinositol-4-phosphate 5-Kinase Ialpha Impairs Localized Actin Remodeling and Suppresses Phagocytosis. J. Biol. Chem. 277: 43849-43857 [Abstract] [Full Text]  
  • Brewer, J. A., Kanagawa, O., Sleckman, B. P., Muglia, L. J. (2002). Thymocyte Apoptosis Induced by T Cell Activation Is Mediated by Glucocorticoids In Vivo. J. Immunol. 169: 1837-1843 [Abstract] [Full Text]  
  • Stenton, G. R., Ulanova, M., Dery, R. E., Merani, S., Kim, M.-K., Gilchrist, M., Puttagunta, L., Musat-Marcu, S., James, D., Schreiber, A. D., Befus, A. D. (2002). Inhibition of Allergic Inflammation in the Airways Using Aerosolized Antisense to Syk Kinase. J. Immunol. 169: 1028-1036 [Abstract] [Full Text]  
  • Scanga, C. A., Aliberti, J., Jankovic, D., Tilloy, F., Bennouna, S., Denkers, E. Y., Medzhitov, R., Sher, A. (2002). Cutting Edge: MyD88 Is Required for Resistance to Toxoplasma gondii Infection and Regulates Parasite-Induced IL-12 Production by Dendritic Cells. J. Immunol. 168: 5997-6001 [Abstract] [Full Text]  
  • Barabe, F., Rollet-Labelle, E., Gilbert, C., Fernandes, M. J. G., Naccache, S. N., Naccache, P. H. (2002). Early Events in the Activation of Fc{gamma}RIIA in Human Neutrophils: Stimulated Insolubilization, Translocation to Detergent-Resistant Domains, and Degradation of Fc{gamma}RIIA. J. Immunol. 168: 4042-4049 [Abstract] [Full Text]  
  • Chi, M., Tridandapani, S., Zhong, W., Coggeshall, K. M., Mortensen, R. F. (2002). C-Reactive Protein Induces Signaling Through Fc{gamma}RIIa on HL-60 Granulocytes. J. Immunol. 168: 1413-1418 [Abstract] [Full Text]  
  • Majeed, M., Caveggion, E., Lowell, C. A., Berton, G. (2001). Role of Src kinases and Syk in Fc{gamma} receptor-mediated phagocytosis and phagosome-lysosome fusion. J. Leukoc. Biol. 70: 801-811 [Abstract] [Full Text]  
  • Yanagi, S., Inatome, R., Ding, J., Kitaguchi, H., Tybulewicz, V. L. J., Yamamura, H. (2001). Syk expression in endothelial cells and their morphologic defects in embryonic Syk-deficient mice. Blood 98: 2869-2871 [Abstract] [Full Text]  
  • Desaulniers, P., Fernandes, M., Gilbert, C., Bourgoin, S. G., Naccache, P. H. (2001). Crystal-induced neutrophil activation. VII. Involvement of Syk in the responses to monosodium urate crystals. J. Leukoc. Biol. 70: 659-668 [Abstract] [Full Text]  
  • Kedzierska, K., Vardaxis, N. J., Jaworowski, A., Crowe, S. M. (2001). Fc{gamma}R-mediated phagocytosis by human macrophages involves Hck, Syk, and Pyk2 and is augmented by GM-CSF. J. Leukoc. Biol. 70: 322-328 [Abstract] [Full Text]  
  • Cooney, D. S., Phee, H., Jacob, A., Coggeshall, K. M. (2001). Signal Transduction by Human-Restricted Fc{{gamma}}RIIa Involves Three Distinct Cytoplasmic Kinase Families Leading to Phagocytosis. J. Immunol. 167: 844-854 [Abstract] [Full Text]  
  • Yamada, T., Fujieda, S., Yanagi, S., Yamamura, H., Inatome, R., Yamamoto, H., Igawa, H., Saito, H. (2001). IL-1 Induced Chemokine Production Through the Association of Syk with TNF Receptor-Associated Factor-6 in Nasal Fibroblast Lines. J. Immunol. 167: 283-288 [Abstract] [Full Text]  
  • Marshall, J. G., Booth, J. W., Stambolic, V., Mak, T., Balla, T., Schreiber, A. D., Meyer, T., Grinstein, S. (2001). Restricted Accumulation of Phosphatidylinositol 3-Kinase Products in a Plasmalemmal Subdomain during Fc{gamma} Receptor-Mediated Phagocytosis. JCB 153: 1369-1380 [Abstract] [Full Text]  
  • Bertagnolo, V., Marchisio, M., Brugnoli, F., Bavelloni, A., Boccafogli, L., Colamussi, M. L., Capitani, S. (2001). Requirement of Tyrosine-phosphorylated Vav for Morphological Differentiation of All-trans-Retinoic Acid-treated HL-60 Cells. Cell Growth Differ. 12: 193-200 [Abstract] [Full Text]  
  • Sada, K., Zhang, J., Siraganian, R. P. (2001). SH2 domain-mediated targeting, but not localization, of Syk in the plasma membrane is critical for Fc{epsilon}RI signaling. Blood 97: 1352-1359 [Abstract] [Full Text]  
  • Kim, C., Dinauer, M. C. (2001). Rac2 Is an Essential Regulator of Neutrophil Nicotinamide Adenine Dinucleotide Phosphate Oxidase Activation in Response to Specific Signaling Pathways. J. Immunol. 166: 1223-1232 [Abstract] [Full Text]  
  • May, R., Machesky, L. (2001). Phagocytosis and the actin cytoskeleton. J. Cell Sci. 114: 1061-1077 [Abstract]  
  • Yamada, T., Fujieda, S., Yanagi, S., Yamamura, H., Inatome, R., Sunaga, H., Saito, H. (2001). Protein-Tyrosine Kinase Syk Expressed in Human Nasal Fibroblasts and Its Effect on RANTES Production. J. Immunol. 166: 538-543 [Abstract] [Full Text]  
  • Botelho, R. J., Teruel, M., Dierckman, R., Anderson, R., Wells, A., York, J. D., Meyer, T., Grinstein, S. (2000). Localized Biphasic Changes in Phosphatidylinositol-4,5-Bisphosphate at Sites of Phagocytosis. JCB 151: 1353-1368 [Abstract] [Full Text]  
  • Judd, B. A., Myung, P. S., Leng, L., Obergfell, A., Pear, W. S., Shattil, S. J., Koretzky, G. A. (2000). Hematopoietic reconstitution of SLP-76 corrects hemostasis and platelet signaling through alpha IIbbeta 3 and collagen receptors. Proc. Natl. Acad. Sci. USA 97: 12056-12061 [Abstract] [Full Text]  
  • Lach-Trifilieff, E., Menear, K., Schweighoffer, E., Tybulewicz, V. L. J., Walker, C. (2000). Syk-deficient eosinophils show normal interleukin-5-mediated differentiation, maturation, and survival but no longer respond to Fcgamma R activation. Blood 96: 2506-2510 [Abstract] [Full Text]  
  • Myung, P. S., Clements, J. L., White, D. W., Malik, Z. A., Cowdery, J. S., Allen, L.-A. H., Harty, J. T., Kusner, D. J., Koretzky, G. A. (2000). In vitro and in vivo macrophage function can occur independently of SLP-76. Int Immunol 12: 887-897 [Abstract] [Full Text]  
  • Aittomaki, S., Pesu, M., Groner, B., Janne, O. A., Palvimo, J. J., Silvennoinen, O. (2000). Cooperation Among Stat1, Glucocorticoid Receptor, and PU.1 in Transcriptional Activation of the High-Affinity Fc{gamma} Receptor I in Monocytes. J. Immunol. 164: 5689-5697 [Abstract] [Full Text]  
  • Colucci, F., Guy-Grand, D., Wilson, A., Turner, M., Schweighoffer, E., Tybulewicz, V. L. J., Di Santo, J. P. (2000). A New Look at Syk in {alpha}{beta} and {gamma}{delta} T Cell Development Using Chimeric Mice with a Low Competitive Hematopoietic Environment. J. Immunol. 164: 5140-5145 [Abstract] [Full Text]  
  • Hazan-Halevy, I., Seger, R., Levy, R. (2000). The Requirement of Both Extracellular Regulated Kinase and p38 Mitogen-activated Protein Kinase for Stimulation of Cytosolic Phospholipase A2 Activity by Either Fcgamma RIIA or Fcgamma RIIIB in Human Neutrophils. A POSSIBLE ROLE FOR Pyk2 BUT NOT FOR THE Grb2-Sos-Shc COMPLEX. J. Biol. Chem. 275: 12416-12423 [Abstract] [Full Text]  
  • Stenton, G. R., Kim, M.-K., Nohara, O., Chen, C.-F., Hirji, N., Wills, F. L., Gilchrist, M., Hwang, P.-H., Park, J.-G., Finlay, W., Jones, R. L., Befus, A. D., Schreiber, A. D. (2000). Aerosolized Syk Antisense Suppresses Syk Expression, Mediator Release from Macrophages, and Pulmonary Inflammation. J. Immunol. 164: 3790-3797 [Abstract] [Full Text]  
  • Fitzer-Attas, C. J., Lowry, M., Crowley, M. T., Finn, A. J., Meng, F., DeFranco, A. L., Lowell, C. A. (2000). Fc{gamma} Receptor-Mediated Phagocytosis in Macrophages Lacking the Src Family Tyrosine Kinases Hck, Fgr, and Lyn. JEM 191: 669-682 [Abstract] [Full Text]  
  • Lin, T.-J., Hirji, N., Stenton, G. R., Gilchrist, M., Grill, B. J., Schreiber, A. D., Befus, A. D. (2000). Activation of Macrophage CD8: Pharmacological Studies of TNF and IL-1{beta} Production. J. Immunol. 164: 1783-1792 [Abstract] [Full Text]  
  • Gresham, H. D., Dale, B. M., Potter, J. W., Chang, P. W., Vines, C. M., Lowell, C. A., Lagenaur, C. F., Willman, C. L. (2000). Negative Regulation of Phagocytosis in Murine Macrophages by the Src Kinase Family Member, Fgr. JEM 191: 515-528 [Abstract] [Full Text]  
  • Faruki, S, Geahlen, R., Asai, D. (2000). Syk-dependent phosphorylation of microtubules in activated B-lymphocytes. J. Cell Sci. 113: 2557-2565 [Abstract]  
  • Raeder, E. M. B., Mansfield, P. J., Hinkovska-Galcheva, V., Shayman, J. A., Boxer, L. A. (1999). Syk Activation Initiates Downstream Signaling Events During Human Polymorphonuclear Leukocyte Phagocytosis. J. Immunol. 163: 6785-6793 [Abstract] [Full Text]  
  • Pasquet, J.-M., Gross, B., Quek, L., Asazuma, N., Zhang, W., Sommers, C. L., Schweighoffer, E., Tybulewicz, V., Judd, B., Lee, J. R., Koretzky, G., Love, P. E., Samelson, L. E., Watson, S. P. (1999). LAT Is Required for Tyrosine Phosphorylation of Phospholipase Cgamma 2 and Platelet Activation by the Collagen Receptor GPVI. Mol. Cell. Biol. 19: 8326-8334 [Abstract] [Full Text]  
  • Sato, N., Kim, M.-K., Schreiber, A. D. (1999). Enhancement of Fc{gamma} Receptor-Mediated Phagocytosis by Transforming Mutants of Cbl. J. Immunol. 163: 6123-6131 [Abstract] [Full Text]  
  • Edberg, J. C., Yee, A. M. F., Rakshit, D. S., Chang, D. J., Gokhale, J. A., Indik, Z. K., Schreiber, A. D., Kimberly, R. P. (1999). The Cytoplasmic Domain of Human Fcgamma RIa Alters the Functional Properties of the Fcgamma RI{middle dot}gamma -Chain Receptor Complex. J. Biol. Chem. 274: 30328-30333 [Abstract] [Full Text]  
  • van Leeuwen, J. E. M., Paik, P. K., Samelson, L. E. (1999). The Oncogenic 70Z Cbl Mutation Blocks the Phosphotyrosine Binding Domain-Dependent Negative Regulation of ZAP-70 by c-Cbl in Jurkat T Cells. Mol. Cell. Biol. 19: 6652-6664 [Abstract] [Full Text]  
  • Downey, G. P., Botelho, R. J., Butler, J. R., Moltyaner, Y., Chien, P., Schreiber, A. D., Grinstein, S. (1999). Phagosomal Maturation, Acidification, and Inhibition of Bacterial Growth in Nonphagocytic Cells Transfected with Fcgamma RIIA Receptors. J. Biol. Chem. 274: 28436-28444 [Abstract] [Full Text]  
  • Zhang, J., Billingsley, M. L., Kincaid, R. L., Siraganian, R. P. (2000). Phosphorylation of Syk Activation Loop Tyrosines Is Essential for Syk Function. AN IN VIVO STUDY USING A SPECIFIC ANTI-Syk ACTIVATION LOOP PHOSPHOTYROSINE ANTIBODY. J. Biol. Chem. 275: 35442-35447 [Abstract] [Full Text]  
  • Cornillon, S., Pech, E., Benghezal, M., Ravanel, K., Gaynor, E., Letourneur, F., Bruckert, F., Cosson, P. (2000). Phg1p Is a Nine-transmembrane Protein Superfamily Member Involved in Dictyostelium Adhesion and Phagocytosis. J. Biol. Chem. 275: 34287-34292 [Abstract] [Full Text]  
  • Kuwae, A., Yoshida, S., Tamano, K., Mimuro, H., Suzuki, T., Sasakawa, C. (2001). Shigella Invasion of Macrophage Requires the Insertion of IpaC into the Host Plasma Membrane. FUNCTIONAL ANALYSIS OF IpaC. J. Biol. Chem. 276: 32230-32239 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»