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Molecular and Cellular Biology, September 1998, p. 5032-5041, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Cellular Repressor of E1A-Stimulated Genes That Inhibits Activation by E2F

Elizabeth Veal,1 Michael Eisenstein,1 Zian H. Tseng,2 and Grace Gill1,*

Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115,1 and Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 947202

Received 18 March 1998/Returned for modification 3 June 1998/Accepted 12 June 1998

The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. Here we report the identification and characterization of a cellular protein that antagonizes transcriptional activation and cellular transformation by E1A. This protein, termed CREG for cellular repressor of E1A-stimulated genes, shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. In transfection assays, CREG represses transcription and antagonizes 12SE1A-mediated activation of both the adenovirus E2 and cellular hsp70 promoters. CREG also antagonizes E1A-mediated transformation, as expression of CREG reduces the efficiency with which E1A and the oncogene ras cooperate to transform primary cells. Binding sites for E2F, a key transcriptional regulator of cell cycle progression, were found to be required for repression of the adenovirus E2 promoter by CREG, and CREG was shown to inhibit activation by E2F. Since both the adenovirus E1A protein and transcriptional activation by E2F function to promote cellular proliferation, the results presented here suggest that CREG activity may contribute to the transcriptional control of cell growth and differentiation.

* Corresponding author. Mailing address: Department of Pathology, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-0985. Fax: (617) 432-1313. E-mail: ggill{at}warren.med.harvard.edu.

Molecular and Cellular Biology, September 1998, p. 5032-5041, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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