This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, S.-C.
Right arrow Articles by Stavnezer, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, S.-C.
Right arrow Articles by Stavnezer, J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 1998, p. 5523-5532, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Ability of CD40L, but Not Lipopolysaccharide, To Initiate Immunoglobulin Switching to Immunoglobulin G1 Is Explained by Differential Induction of NF-kappa B/Rel Proteins

Shih-Chang Lin,1 Henry H. Wortis,2 and Janet Stavnezer1,*

Department of Molecular Genetics and Microbiology and Program in Immunology and Virology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0122,1 and Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111-18002

Received 14 November 1997/Returned for modification 13 January 1998/Accepted 25 June 1998

Antibodies of the immunoglobulin G1 class are induced in mice by T-cell-dependent antigens but not by lipopolysaccharide (LPS). CD40 engagement contributes to this preferential isotype production by activating NF-kappa B/Rel to induce germ line gamma 1 transcripts, which are essential for class switch recombination. Although LPS also activates NF-kappa B, it poorly induces germ line gamma 1 transcripts. Western blot analyses show that CD40 ligand (CD40L) induces all NF-kappa B/Rel proteins, whereas LPS activates predominantly p50 and c-Rel. Electrophoretic mobility shift assays show that in CD40L-treated cells, p50-RelA and p50-RelB dimers are the major NF-kappa B complexes binding to the germ line gamma 1 promoter, whereas in LPS-treated cells, p50-c-Rel and p50-p50 dimers are the major binding complexes. Transfection of expression plasmids for NF-kappa B/Rel fusion proteins (forced dimers) indicates that p50-RelA and p50-RelB dimers activate the germ line gamma 1 promoter and that p50-c-Rel and p50-p50 dimers inhibit this activation by competitively binding to the promoter without activating the promoter. Therefore, germ line gamma 1 transcription depends on the composition of NF-kappa B/Rel proteins.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology and Program in Immunology and Virology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655-0122. Phone: (508) 856-4100. Fax: (508) 856-1789. E-mail: janet.stavnezer{at}banyan.ummed.edu.

Molecular and Cellular Biology, September 1998, p. 5523-5532, Vol. 18, No. 9
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Baglole, C. J., Maggirwar, S. B., Gasiewicz, T. A., Thatcher, T. H., Phipps, R. P., Sime, P. J. (2008). The Aryl Hydrocarbon Receptor Attenuates Tobacco Smoke-induced Cyclooxygenase-2 and Prostaglandin Production in Lung Fibroblasts through Regulation of the NF-{kappa}B Family Member RelB. J. Biol. Chem. 283: 28944-28957 [Abstract] [Full Text]  
  • Kin, N. W., Sanders, V. M. (2007). CD86 Regulates IgG1 Production via a CD19-Dependent Mechanism. J. Immunol. 179: 1516-1523 [Abstract] [Full Text]  
  • Shen, L., Zhang, C., Wang, T., Brooks, S., Ford, R. J., Lin-Lee, Y. C., Kasianowicz, A., Kumar, V., Martin, L., Liang, P., Cowell, J., Ambrus, J. L. Jr (2006). Development of Autoimmunity in IL-14{alpha}-Transgenic Mice. J. Immunol. 177: 5676-5686 [Abstract] [Full Text]  
  • Kin, N. W., Sanders, V. M. (2006). CD86 Stimulation on a B Cell Activates the Phosphatidylinositol 3-Kinase/Akt and Phospholipase C{gamma}2/Protein Kinase C{alpha}beta Signaling Pathways.. J. Immunol. 176: 6727-6735 [Abstract] [Full Text]  
  • Chang, P.-Y., Miyamoto, S. (2006). Nuclear Factor-{kappa}B Dimer Exchange Promotes a p21waf1/cip1 Superinduction Response in Human T Leukemic Cells. Mol Cancer Res 4: 101-112 [Abstract] [Full Text]  
  • Berton, M. T., Linehan, L. A., Wick, K. R., Dunnick, W. A. (2004). NF-{kappa}B elements associated with the Stat6 site in the germline {gamma}1 immunoglobulin promoter are not necessary for the transcriptional response to CD40 ligand. Int Immunol 16: 1741-1749 [Abstract] [Full Text]  
  • Podojil, J. R., Kin, N. W., Sanders, V. M. (2004). CD86 and {beta}2-Adrenergic Receptor Signaling Pathways, Respectively, Increase Oct-2 and OCA-B Expression and Binding to the 3'-IgH Enhancer in B Cells. J. Biol. Chem. 279: 23394-23404 [Abstract] [Full Text]  
  • Elewaut, D., Shaikh, R. B., Hammond, K. J. L., De Winter, H., Leishman, A. J., Sidobre, S., Turovskaya, O., Prigozy, T. I., Ma, L., Banks, T. A., Lo, D., Ware, C. F., Cheroutre, H., Kronenberg, M. (2003). NIK-dependent RelB Activation Defines a Unique Signaling Pathway for the Development of V{alpha}14i NKT Cells. JEM 197: 1623-1633 [Abstract] [Full Text]  
  • Bhattacharya, D., Lee, D. U., Sha, W. C. (2002). Regulation of Ig class switch recombination by NF-{kappa}B: retroviral expression of RelB in activated B cells inhibits switching to IgG1, but not to IgE. Int Immunol 14: 983-991 [Abstract] [Full Text]  
  • Pizzi, M., Goffi, F., Boroni, F., Benarese, M., Perkins, S. E., Liou, H.-C., Spano, P. (2002). Opposing Roles for NF-kappa B/Rel Factors p65 and c-Rel in the Modulation of Neuron Survival Elicited by Glutamate and Interleukin-1beta. J. Biol. Chem. 277: 20717-20723 [Abstract] [Full Text]  
  • Gelfanov, V. M., Burgess, G. S., Litz-Jackson, S., King, A. J., Marshall, M. S., Nakshatri, H., Boswell, H. S. (2001). Transformation of interleukin-3-dependent cells without participation of Stat5/bcl-xL: cooperation of akt with raf/erk leads to p65 nuclear factor {kappa}B-mediated antiapoptosis involving c-IAP2. Blood 98: 2508-2517 [Abstract] [Full Text]  
  • Gao, N., Dang, T., Yuan, D. (2001). IFN-{gamma}-Dependent and -Independent Initiation of Switch Recombination by NK Cells. J. Immunol. 167: 2011-2018 [Abstract] [Full Text]  
  • Mao, C.-s., Stavnezer, J. (2001). Differential Regulation of Mouse Germline Ig {gamma}1 and {epsilon} Promoters by IL-4 and CD40. J. Immunol. 167: 1522-1534 [Abstract] [Full Text]  
  • Shi, M.-J., Park, S.-R., Kim, P.-H., Stavnezer, J. (2001). Roles of Ets proteins, NF-{{kappa}}B and nocodazole in regulating induction of transcription of mouse germline Ig {{alpha}} RNA by transforming growth factor-{beta}1. Int Immunol 13: 733-746 [Abstract] [Full Text]  
  • Wuerffel, R. A., Ma, L., Kenter, A. L. (2001). NF-{{kappa}}B p50-Dependent In Vivo Footprints at Ig S{{gamma}}3 DNA Are Correlated with {micro}{->}{{gamma}}3 Switch Recombination. J. Immunol. 166: 4552-4559 [Abstract] [Full Text]  
  • Zelazowski, P., Shen, Y., Snapper, C. M. (2000). NF-{kappa}B/p50 and NF-{kappa}B/c-Rel differentially regulate the activity of the 3'{alpha}E-hsl,2 enhancer in normal murine B cells in an activation-dependent manner. Int Immunol 12: 1167-1172 [Abstract] [Full Text]  
  • Bajpai, U. D., Zhang, K., Teutsch, M., Sen, R., Wortis, H. H. (2000). Bruton's Tyrosine Kinase Links the B Cell Receptor to Nuclear Factor {kappa}b Activation. JEM 191: 1735-1744 [Abstract] [Full Text]  
  • Bhushan, A., Barnhart, B., Shone, S., Song, C., Covey, L. R. (2000). A Transcriptional Defect Underlies B Lymphocyte Dysfunction in a Patient Diagnosed with Non-X-Linked Hyper-IgM Syndrome. J. Immunol. 164: 2871-2880 [Abstract] [Full Text]  
  • McRae, B. L., Beilfuss, B. A., Seventer, G. A. v. (2000). IFN-{beta} Differentially Regulates CD40-Induced Cytokine Secretion by Human Dendritic Cells. J. Immunol. 164: 23-28 [Abstract] [Full Text]  
  • Sedger, L. M., Shows, D. M., Blanton, R. A., Peschon, J. J., Goodwin, R. G., Cosman, D., Wiley, S. R. (1999). IFN-{gamma} Mediates a Novel Antiviral Activity Through Dynamic Modulation of TRAIL and TRAIL Receptor Expression. J. Immunol. 163: 920-926 [Abstract] [Full Text]  
  • Hsing, Y., Bishop, G. A. (1999). Requirement for Nuclear Factor-{kappa}B Activation by a Distinct Subset of CD40-Mediated Effector Functions in B Lymphocytes. J. Immunol. 162: 2804-2811 [Abstract] [Full Text]  
  • Kasibhatla, S., Genestier, L., Green, D. R. (1999). Regulation of Fas-Ligand Expression during Activation-induced Cell Death in T Lymphocytes via Nuclear Factor kappa B. J. Biol. Chem. 274: 987-992 [Abstract] [Full Text]  
  • Solan, N. J., Miyoshi, H., Carmona, E. M., Bren, G. D., Paya, C. V. (2002). RelB Cellular Regulation and Transcriptional Activity Are Regulated by p100. J. Biol. Chem. 277: 1405-1418 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»