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Molecular and Cellular Biology, January 1999, p. 392-401, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The B29 (Immunoglobulin beta -Chain) Gene Is a Genetic Target for Early B-Cell Factor

Peter Åkerblad, Maria Rosberg, Tomas Leanderson, and Mikael Sigvardsson*

Immunology Group, CMB, Lund University, S-223 62 Lund, Sweden

Received 3 June 1998/Returned for modification 30 June 1998/Accepted 17 September 1998

Early B-cell factor (EBF) is a transcription factor suggested as essential for early B-lymphocyte development by findings in mice where the coding gene has been inactivated by homologous disruption. This makes the identification of genetic targets for this transcription factor pertinent for the understanding of early B-cell development. The lack of B29 transcripts, coding for the beta  subunit of the B-cell receptor complex, in pro-B cells from EBF-deficient mice suggested that B29 might be a genetic target for EBF. We here present data suggesting that EBF interacts with three independent sites within the mouse B29 promoter. Furthermore, ectopic expression of EBF in HeLa cells activated a B29 promoter-controlled reporter construct 13-fold and induced a low level of expression from the endogenous B29 gene. Finally, mutations in the EBF binding sites diminished B29 promoter activity in pre-B cells while the same mutations did not have as striking an effect on the promoter function in B-cell lines of later differentiation stages. These data suggest that the B29 gene is a genetic target for EBF in early B-cell development.

* Corresponding author. Mailing address: Immunology Group, CMB, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden. Phone: 46 462224160. Fax: 46 462224218. E-mail: mikael.sigvardsson{at}immuno.lu.se.

Molecular and Cellular Biology, January 1999, p. 392-401, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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