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Molecular and Cellular Biology, October 1999, p. 6543-6553, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic and Physical Interactions Involving the Yeast Nuclear Cap-Binding Complex

Puri Fortes,1 Joanna Kufel,2 Maarten Fornerod,1 Maria Polycarpou-Schwarz,1 Denis Lafontaine,2 David Tollervey,2 and Iain W. Mattaj1,*

European Molecular Biology Laboratory, D-69117 Heidelberg, Germany,1 and Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, United Kingdom2

Received 31 March 1999/Returned for modification 14 May 1999/Accepted 12 July 1999

Yeast strains lacking the yeast nuclear cap-binding complex (yCBC) are viable, although impaired in growth. We have taken advantage of this observation to carry out a genetic screen for components that show synthetic lethality (SL) with a cbp20-Delta cbp80-Delta double mutation. One set of SL interactions was due to mutations that were complemented by components of U1 small nuclear RNP (snRNP) and the yeast splicing commitment complex. These interactions confirm the role of yCBC in commitment complex formation. Physical interaction of yCBC with the commitment complex components Mud10p and Mud2p, which may directly mediate yCBC function, was demonstrated. Unexpectedly, we identified multiple SL mutations that were complemented by Cbf5p and Nop58p. These are components of the two major classes of yeast small nucleolar RNPs, which function in the maturation of rRNA precursors. Mutants lacking yCBC were found to be defective in rRNA processing. Analysis of the yCBC deletion phenotype suggests that this is likely to be due to a defect in the splicing of a subset of ribosomal protein mRNA precursors.

* Corresponding author. Mailing address: European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. Phone: 49 6221 387 393. Fax: 49 6221 387 518. E-mail: mattaj{at}embl-heidelberg.de.

Molecular and Cellular Biology, October 1999, p. 6543-6553, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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