This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kirkpatrick, D. T.
Right arrow Articles by Petes, T. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirkpatrick, D. T.
Right arrow Articles by Petes, T. D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 1999, p. 7661-7671, Vol. 19, No. 11
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Control of Meiotic Recombination and Gene Expression in Yeast by a Simple Repetitive DNA Sequence That Excludes Nucleosomes

David T. Kirkpatrick,1 Yuh-Hwa Wang,2,3 Margaret Dominska,1 Jack D. Griffith,2 and Thomas D. Petes1,*

Department of Biology and Curriculum in Genetics and Molecular Biology1 and The Lineberger Comprehensive Cancer Center,2 University of North Carolina, Chapel Hill, North Carolina 27599-3280, and Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 088543

Received 19 May 1999/Returned for modification 29 June 1999/Accepted 30 July 1999

Tandem repeats of the pentanucleotide 5'-CCGNN (where N indicates any base) were previously shown to exclude nucleosomes in vitro (Y.-H. Wang and J. D. Griffith, Proc. Natl. Acad. Sci. USA 93:8863-8867, 1996). To determine the in vivo effects of these sequences, we replaced the upstream regulatory sequences of the HIS4 gene of Saccharomyces cerevisiae with either 12 or 48 tandem copies of CCGNN. Both tracts activated HIS4 transcription. We found that (CCGNN)12 tracts elevated meiotic recombination (hot spot activity), whereas the (CCGNN)48 tract repressed recombination (cold spot activity). In addition, a "pure" tract of (CCGAT)12 activated both transcription and meiotic recombination. We suggest that the cold spot activity of the (CCGNN)48 tract is related to the phenomenon of the suppressive interactions of adjacent hot spots previously described in yeast (Q.-Q. Fan, F. Xu, and T. D. Petes, Mol. Cell. Biol. 15:1679-1688, 1995; Q.-Q. Fan, F. Xu, M. A. White, and T. D. Petes, Genetics 145:661-670, 1997; T.-C. Wu and M. Lichten, Genetics 140:55-66, 1995; L. Xu and N. Kleckner, EMBO J. 16:5115-5128, 1995).

* Corresponding author. Mailing address: Department of Biology, University of North Carolina at Chapel Hill, CB no. 3280, Coker Hall, Chapel Hill, NC 27599-3280. Phone: (919) 962-1445. Fax: (919) 962-8472. E-mail: tompetes{at}emailunc.edu.

Molecular and Cellular Biology, November 1999, p. 7661-7671, Vol. 19, No. 11
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Steiner, W. W., Steiner, E. M., Girvin, A. R., Plewik, L. E. (2009). Novel Nucleotide Sequence Motifs That Produce Hotspots of Meiotic Recombination in Schizosaccharomyces pombe. Genetics 182: 459-469 [Abstract] [Full Text]  
  • Brandstrom, M., Bagshaw, A. T., Gemmell, N. J., Ellegren, H. (2008). The Relationship Between Microsatellite Polymorphism and Recombination Hot Spots in the Human Genome. Mol Biol Evol 25: 2579-2587 [Abstract] [Full Text]  
  • Fukuda, T., Kugou, K., Sasanuma, H., Shibata, T., Ohta, K. (2008). Targeted induction of meiotic double-strand breaks reveals chromosomal domain-dependent regulation of Spo11 and interactions among potential sites of meiotic recombination. Nucleic Acids Res 36: 984-997 [Abstract] [Full Text]  
  • Tsai, C. J., Mets, D. G., Albrecht, M. R., Nix, P., Chan, A., Meyer, B. J. (2008). Meiotic crossover number and distribution are regulated by a dosage compensation protein that resembles a condensin subunit. Genes Dev. 22: 194-211 [Abstract] [Full Text]  
  • Yu, Q., Sandmeier, J., Xu, H., Zou, Y., Bi, X. (2006). Mechanism of the Long Range Anti-silencing Function of Targeted Histone Acetyltransferases in Yeast. J. Biol. Chem. 281: 3980-3988 [Abstract] [Full Text]  
  • Mieczkowski, P. A., Dominska, M., Buck, M. J., Gerton, J. L., Lieb, J. D., Petes, T. D. (2006). Global Analysis of the Relationship between the Binding of the Bas1p Transcription Factor and Meiosis-Specific Double-Strand DNA Breaks in Saccharomyces cerevisiae. Mol. Cell. Biol. 26: 1014-1027 [Abstract] [Full Text]  
  • Wells, R. D., Dere, R., Hebert, M. L., Napierala, M., Son, L. S. (2005). Advances in mechanisms of genetic instability related to hereditary neurological diseases. Nucleic Acids Res 33: 3785-3798 [Abstract] [Full Text]  
  • Steiner, W. W., Smith, G. R. (2005). Optimizing the Nucleotide Sequence of a Meiotic Recombination Hotspot in Schizosaccharomyces pombe. Genetics 169: 1973-1983 [Abstract] [Full Text]  
  • Mulvihill, D. J., Edamura, K. N., Hagerman, K. A., Pearson, C. E., Wang, Y.-H. (2005). Effect of CAT or AGG Interruptions and CpG Methylation on Nucleosome Assembly upon Trinucleotide Repeats on Spinocerebellar Ataxia, Type 1 and Fragile X Syndrome. J. Biol. Chem. 280: 4498-4503 [Abstract] [Full Text]  
  • Bi, X., Yu, Q., Sandmeier, J. J., Zou, Y. (2004). Formation of Boundaries of Transcriptionally Silent Chromatin by Nucleosome-Excluding Structures. Mol. Cell. Biol. 24: 2118-2131 [Abstract] [Full Text]  
  • Napierala, M., Dere, R., Vetcher, A., Wells, R. D. (2004). Structure-dependent Recombination Hot Spot Activity of GAA{middle dot}TTC Sequences from Intron 1 of the Friedreich's Ataxia Gene. J. Biol. Chem. 279: 6444-6454 [Abstract] [Full Text]  
  • Petes, T. D., Merker, J. D. (2002). Context Dependence of Meiotic Recombination Hotspots in Yeast: The Relationship Between Recombination Activity of a Reporter Construct and Base Composition. Genetics 162: 2049-2052 [Abstract] [Full Text]  
  • Hsu, Y. Y., Wang, Y.-H. (2002). Human Fragile Site FRA16B DNA Excludes Nucleosomes in the Presence of Distamycin. J. Biol. Chem. 277: 17315-17319 [Abstract] [Full Text]  
  • Jauert, P. A., Edmiston, S. N., Conway, K., Kirkpatrick, D. T. (2002). RAD1 Controls the Meiotic Expansion of the Human HRAS1 Minisatellite in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 953-964 [Abstract] [Full Text]  
  • Abdullah, M. F. F., Borts, R. H. (2001). Meiotic recombination frequencies are affected by nutritional states in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 10.1073/pnas.201529598v1 [Abstract] [Full Text]  
  • Kirkpatrick, D. T., Ferguson, J. R., Petes, T. D., Symington, L. S. (2000). Decreased Meiotic Intergenic Recombination and Increased Meiosis I Nondisjunction in exo1 Mutants of Saccharomyces cerevisiae. Genetics 156: 1549-1557 [Abstract] [Full Text]  
  • Gerton, J. L., DeRisi, J., Shroff, R., Lichten, M., Brown, P. O., Petes, T. D. (2000). Inaugural Article: Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 97: 11383-11390 [Abstract] [Full Text]  
  • Yesland, K., Fonzi, W. A. (2000). Allele-specific gene targeting in Candida albicans results from heterology between alleles. Microbiology 146: 2097-2104 [Abstract] [Full Text]  
  • Nasar, F., Jankowski, C., Nag, D. K. (2000). Long Palindromic Sequences Induce Double-Strand Breaks during Meiosis in Yeast. Mol. Cell. Biol. 20: 3449-3458 [Abstract] [Full Text]  
  • Abdullah, M. F. F., Borts, R. H. (2001). Meiotic recombination frequencies are affected by nutritional states in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 98: 14524-14529 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»