This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhao, J.
Right arrow Articles by Moore, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, J.
Right arrow Articles by Moore, C.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 1999, p. 7733-7740, Vol. 19, No. 11
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Pta1, a Component of Yeast CF II, Is Required for Both Cleavage and Poly(A) Addition of mRNA Precursor

Jing Zhao,1 Marco Kessler,1 Steffen Helmling,2 J. Patrick O'Connor,3 and Claire Moore1,2,*

Department of Molecular Biology and Microbiology1and Department of Biochemistry,2 School of Medicine, Tufts University, Boston, Massachusetts, and Department of Orthopaedics, UMDNJ-New Jersey Medical School, Newark, New Jersey3

Received 13 May 1999/Returned for modification 23 June 1999/Accepted 23 August 1999

CF II, a factor required for cleavage of the 3' ends of mRNA precursor in Saccharomyces cerevisiae, has been shown to contain four polypeptides. The three largest subunits, Cft1/Yhh1, Cft2/Ydh1, and Brr5/Ysh1, are homologs of the three largest subunits of mammalian cleavage-polyadenylation specificity factor (CPSF), an activity needed for both cleavage and poly(A) addition. In this report, we show by protein sequencing and immunoreactivity that the fourth subunit of CF II is Pta1, an essential 90-kDa protein originally implicated in tRNA splicing. Yth1, the yeast homolog of the CPSF 30-kDa subunit, is not detected in this complex. Extracts prepared from pta1 mutant strains are impaired in the cleavage and the poly(A) addition of both GAL7 and CYC1 substrates and exhibit little processing activity even after prolonged incubation. However, activity is efficiently rescued by the addition of purified CF II to the defective extracts. Extract from a strain with a mutation in the CF IA subunit Rna14 also restored processing, but extract from a brr5-1 strain did not. The amounts of Pta1 and other CF II subunits are reduced in pta1 strains, suggesting that levels of the subunits may be coordinately regulated. Coimmunoprecipitation experiments indicate that the CF II in extract can be found in a stable complex containing Pap1, CF II, and the Fip1 and Yth1 subunits of polyadenylation factor I. While purified CF II does not appear to retain the association with these other factors, this larger complex may be the form recruited onto pre-mRNA in vivo. The involvement of Pta1 in both steps of mRNA 3'-end formation supports the conclusion that CF II is the functional homolog of CPSF.

* Corresponding author. Mailing address: Department of Molecular Biology and Microbiology, School of Medicine, Tufts University, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6935. Fax: (617) 636-0337. E-mail: cmoore{at}opal.tufts.edu.

Molecular and Cellular Biology, November 1999, p. 7733-7740, Vol. 19, No. 11
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Qu, X., Lykke-Andersen, S., Nasser, T., Saguez, C., Bertrand, E., Jensen, T. H., Moore, C. (2009). Assembly of an Export-Competent mRNP Is Needed for Efficient Release of the 3'-End Processing Complex after Polyadenylation. Mol. Cell. Biol. 29: 5327-5338 [Abstract] [Full Text]  
  • Roth, K. M., Byam, J., Fang, F., Butler, J. S. (2009). Regulation of NAB2 mRNA 3'-end formation requires the core exosome and the Trf4p component of the TRAMP complex. RNA 15: 1045-1058 [Abstract] [Full Text]  
  • Krishnamurthy, S., Ghazy, M. A., Moore, C., Hampsey, M. (2009). Functional Interaction of the Ess1 Prolyl Isomerase with Components of the RNA Polymerase II Initiation and Termination Machineries. Mol. Cell. Biol. 29: 2925-2934 [Abstract] [Full Text]  
  • Ghazy, M. A., He, X., Singh, B. N., Hampsey, M., Moore, C. (2009). The Essential N Terminus of the Pta1 Scaffold Protein Is Required for snoRNA Transcription Termination and Ssu72 Function but Is Dispensable for Pre-mRNA 3'-End Processing. Mol. Cell. Biol. 29: 2296-2307 [Abstract] [Full Text]  
  • Bucheli, M. E., He, X., Kaplan, C. D., Moore, C. L., Buratowski, S. (2007). Polyadenylation site choice in yeast is affected by competition between Npl3 and polyadenylation factor CFI. RNA 13: 1756-1764 [Abstract] [Full Text]  
  • Atanasiu, D., Fraser, N. W. (2007). The Stable 2-Kilobase Latency-Associated Transcript of Herpes Simplex Virus Type 1 Can Alter the Assembly of the 60S Ribosomal Subunit and Is Exported from Nucleus to Cytoplasm by a CRM1-Dependent Pathway. J. Virol. 81: 7695-7701 [Abstract] [Full Text]  
  • ZHELKOVSKY, A., TACAHASHI, Y., NASSER, T., HE, X., STERZER, U., JENSEN, T. H., DOMDEY, H., MOORE, C. (2006). The role of the Brr5/Ysh1 C-terminal domain and its homolog Syc1 in mRNA 3'-end processing in Saccharomyces cerevisiae. RNA 12: 435-445 [Abstract] [Full Text]  
  • Gilmartin, G. M. (2005). Eukaryotic mRNA 3' processing: a common means to different ends. Genes Dev. 19: 2517-2521 [Full Text]  
  • St-Pierre, B., Liu, X., Kha, L.-C. T., Zhu, X., Ryan, O., Jiang, Z., Zacksenhaus, E. (2005). Conserved and specific functions of mammalian ssu72. Nucleic Acids Res 33: 464-477 [Abstract] [Full Text]  
  • RYAN, K., CALVO, O., MANLEY, J. L. (2004). Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease. RNA 10: 565-573 [Abstract] [Full Text]  
  • Cheng, H., He, X., Moore, C. (2004). The Essential WD Repeat Protein Swd2 Has Dual Functions in RNA Polymerase II Transcription Termination and Lysine 4 Methylation of Histone H3. Mol. Cell. Biol. 24: 2932-2943 [Abstract] [Full Text]  
  • Hendriks, E. F., Abdul-Razak, A., Matthews, K. R. (2003). tbCPSF30 Depletion by RNA Interference Disrupts Polycistronic RNA Processing in Trypanosoma brucei. J. Biol. Chem. 278: 26870-26878 [Abstract] [Full Text]  
  • Calvo, O., Manley, J. L. (2003). Strange bedfellows: polyadenylation factors at the promoter. Genes Dev. 17: 1321-1327 [Full Text]  
  • He, X., Khan, A. U., Cheng, H., Pappas, D. L. Jr., Hampsey, M., Moore, C. L. (2003). Functional interactions between the transcription and mRNA 3' end processing machineries mediated by Ssu72 and Sub1. Genes Dev. 17: 1030-1042 [Abstract] [Full Text]  
  • Tacahashi, Y., Helmling, S., Moore, C. L. (2003). Functional dissection of the zinc finger and flanking domains of the Yth1 cleavage/polyadenylation factor. Nucleic Acids Res 31: 1744-1752 [Abstract] [Full Text]  
  • Hammell, C. M., Gross, S., Zenklusen, D., Heath, C. V., Stutz, F., Moore, C., Cole, C. N. (2002). Coupling of Termination, 3' Processing, and mRNA Export. Mol. Cell. Biol. 22: 6441-6457 [Abstract] [Full Text]  
  • Hofmann, I., Schnolzer, M., Kaufmann, I., Franke, W. W. (2002). Symplekin, a Constitutive Protein of Karyo- and Cytoplasmic Particles Involved in mRNA Biogenesis in Xenopus laevis Oocytes. Mol. Biol. Cell 13: 1665-1676 [Abstract] [Full Text]  
  • Vo, L. T. A., Minet, M., Schmitter, J.-M., Lacroute, F., Wyers, F. (2001). Mpe1, a Zinc Knuckle Protein, Is an Essential Component of Yeast Cleavage and Polyadenylation Factor Required for the Cleavage and Polyadenylation of mRNA. Mol. Cell. Biol. 21: 8346-8356 [Abstract] [Full Text]  
  • Gross, S., Moore, C. L. (2001). Rna15 Interaction with the A-Rich Yeast Polyadenylation Signal Is an Essential Step in mRNA 3'-End Formation. Mol. Cell. Biol. 21: 8045-8055 [Abstract] [Full Text]  
  • Helmling, S., Zhelkovsky, A., Moore, C. L. (2001). Fip1 Regulates the Activity of Poly(A) Polymerase through Multiple Interactions. Mol. Cell. Biol. 21: 2026-2037 [Abstract] [Full Text]  
  • Hirose, Y., Manley, J. L. (2000). RNA polymerase II and the integration of nuclear events. Genes Dev. 14: 1415-1429 [Full Text]  
  • Takagaki, Y., Manley, J. L. (2000). Complex Protein Interactions within the Human Polyadenylation Machinery Identify a Novel Component. Mol. Cell. Biol. 20: 1515-1525 [Abstract] [Full Text]  
  • Rodriguez, C. R., Cho, E.-J., Keogh, M.-C., Moore, C. L., Greenleaf, A. L., Buratowski, S. (2000). Kin28, the TFIIH-Associated Carboxy-Terminal Domain Kinase, Facilitates the Recruitment of mRNA Processing Machinery to RNA Polymerase II. Mol. Cell. Biol. 20: 104-112 [Abstract] [Full Text]  
  • Barilla, D., Lee, B. A., Proudfoot, N. J. (2001). Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 98: 445-450 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»