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Molecular and Cellular Biology, December 1999, p. 8028-8032, Vol. 19, No. 12
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Immunological Development and Cardiovascular Function Are Normal in Annexin VI Null Mutant Mice

Tim E. Hawkins,1 Jürgen Roes,2 Daryl Rees,3 Jayne Monkhouse,3 and Stephen E. Moss1,*

Department of Physiology, University College London, London WC1E 6BT,1 Department of Medicine, Windeyer Institute of Biomedical Sciences, University College London, London W1P 6DB,2 and Wolfson Institute for Biomedical Research and Centre for Clinical Pharmacology, University College London, London WC1E 6JJ,3 United Kingdom

Received 18 August 1999/Accepted 25 August 1999

Annexins are calcium-binding proteins of unknown function but which are implicated in important cellular processes, including anticoagulation, ion flux regulation, calcium homeostasis, and endocytosis. To gain insight into the function of annexin VI, we performed targeted disruption of its gene in mice. Matings between heterozygous mice produced offspring with a normal Mendelian pattern of inheritance, indicating that the loss of annexin VI did not interfere with viability in utero. Mice lacking annexin VI reached sexual maturity at the same age as their normal littermates, and both males and females were fertile. Because of interest in the role of annexin VI in cardiovascular function, we examined heart rate and blood pressure in knockout and wild-type mice and found these to be identical in the two groups. Similarly, the cardiovascular responses of both sets of mice to septic shock were indistinguishable. We also examined components of the immune system and found no differences in thymic, splenic, or bone marrow lymphocyte levels between knockout and wild-type mice. This is the first study of annexin knockout mice, and the lack of a clear phenotype has broad implications for current views of annexin function.

* Corresponding author. Mailing address: Department of Physiology, University College London, Gower St., London WC1E 6BT, United Kingdom. Phone: 0171 380 7744. Fax: 0171 413 8395. E-mail: s.moss{at}ucl.ac.uk.

Molecular and Cellular Biology, December 1999, p. 8028-8032, Vol. 19, No. 12
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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