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Molecular and Cellular Biology, February 1999, p. 1251-1261, Vol. 19, No. 2
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Definition of Transcriptional Pause Elements in Fission Yeast

Agustín Aranda and Nick J. Proudfoot*

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom

Received 9 September 1998/Returned for modification 19 October 1998/Accepted 1 November 1998

Downstream elements (DSEs) with transcriptional pausing activity play an important role in transcription termination of RNA polymerase II. We have defined two such DSEs in Schizosaccharomyces pombe, one for the ura4 gene and a new one in the 3'-end region of the nmt2 gene. Although these DSEs do not have sequence homology, both are orientation specific and are composed of multiple and redundant sequence elements that work together to achieve full pausing activity. Previous studies on the nmt1 and nmt2 genes revealed that transcription extends several kilobases past the genes' poly(A) sites. We show that the insertion of either DSE immediately downstream of the nmt1 poly(A) site induces more immediate termination. nmt2 termination efficiency can be increased by moving the DSE closer to the poly(A) site. These results suggest that DSEs may be a common feature in yeast genes.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, South Parks Rd., Oxford OX1 3RE, United Kingdom. Phone: 44 1865 275566. Fax: 44 1865 275556. E-mail: Nicholas.Proudfoot{at}path.ox.ac.uk.

Molecular and Cellular Biology, February 1999, p. 1251-1261, Vol. 19, No. 2
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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