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Molecular and Cellular Biology, March 1999, p. 1661-1672, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

p50cdc37 Acting in Concert with Hsp90 Is Required for Raf-1 Functiondagger

Nicholas Grammatikakis,1,* Jun-Hsiang Lin,2 Aliki Grammatikakis,1 Philip N. Tsichlis,2 and Brent H. Cochran1

Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111,1 and Fox Chase Cancer Center, Philadelphia, Pennsylvania 191112

Received 22 July 1998/Returned for modification 1 September 1998/Accepted 25 November 1998

Genetic screens in Drosophila have identified p50cdc37 to be an essential component of the sevenless receptor/mitogen-activated kinase protein (MAPK) signaling pathway, but neither the function nor the target of p50cdc37 in this pathway has been defined. In this study, we examined the role of p50cdc37 and its Hsp90 chaperone partner in Raf/Mek/MAPK signaling biochemically. We found that coexpression of wild-type p50cdc37 with Raf-1 resulted in robust and dose-dependent activation of Raf-1 in Sf9 cells. In addition, p50cdc37 greatly potentiated v-Src-mediated Raf-1 activation. Moreover, we found that p50cdc37 is the primary determinant of Hsp90 recruitment to Raf-1. Overexpression of a p50cdc37 mutant which is unable to recruit Hsp90 into the Raf-1 complex inhibited Raf-1 and MAPK activation by growth factors. Similarly, pretreatment with geldanamycin (GA), an Hsp90-specific inhibitor, prevented both the association of Raf-1 with the p50cdc37-Hsp90 heterodimer and Raf-1 kinase activation by serum. Activation of Raf-1 via baculovirus coexpression with oncogenic Src or Ras in Sf9 cells was also strongly inhibited by dominant negative p50cdc37 or by GA. Thus, formation of a ternary Raf-1-p50cdc37-Hsp90 complex is crucial for Raf-1 activity and MAPK pathway signaling. These results provide the first biochemical evidence for the requirement of the p50cdc37-Hsp90 complex in protein kinase regulation and for Raf-1 function in particular.

* Corresponding author. Mailing address: Department of Physiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. E-mail: ngrammat{at}earthlink.net.

dagger N.G. dedicates this paper to John, George, and Bill.

Molecular and Cellular Biology, March 1999, p. 1661-1672, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Lin, J.-H., Makris, A., McMahon, C., Bear, S. E., Patriotis, C., Prasad, V. R., Brent, R., Golemis, E. A., Tsichlis, P. N. (1999). The Ankyrin Repeat-containing Adaptor Protein Tvl-1 Is a Novel Substrate and Regulator of Raf-1. J. Biol. Chem. 274: 14706-14715 [Abstract] [Full Text]  
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