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Molecular and Cellular Biology, March 1999, p. 2000-2007, Vol. 19, No. 3
0270-7306/99

The 3'right-arrow 5' Exonucleases of DNA Polymerases delta  and epsilon  and the 5'right-arrow 3' Exonuclease Exo1 Have Major Roles in Postreplication Mutation Avoidance in Saccharomyces cerevisiae

Hiep T. Tran,1,dagger Dmitry A. Gordenin,1,2 and Michael A. Resnick1,*

Chromosome Stability Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709,1 and Department of Genetics, St. Petersburg State University, St. Petersburg 19034, Russia2

Received 1 July 1998/Returned for modification 5 August 1998/Accepted 18 November 1998

Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5'right-arrow3' Exo1 and the 3'right-arrow5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase varepsilon  (pol2-4) and delta  (pol3-01) mutants defective in 3'right-arrow5' proofreading exonuclease, mutants defective in the 5'right-arrow3' exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes.

* Corresponding author. Mailing address: Chromosome Stability Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences (NIEHS), 101 TW Alexander Dr., P.O. Box 12233, Research Triangle Park, NC 27709. Phone: (919) 541-4480. Fax: (919) 541-7593. E-mail: resnick{at}niehs.nih.gov.

dagger Present address: Life Sensors Inc., Malvern, PA 19355.

Molecular and Cellular Biology, March 1999, p. 2000-2007, Vol. 19, No. 3
0270-7306/99


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