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Molecular and Cellular Biology, March 1999, p. 2088-2097, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Stable Remodeling of Tailless Nucleosomes by the Human SWI-SNF Complexdagger

Jeffrey R. Guyon,1,2,3 Geeta J. Narlikar,1,2 Saïd Sif,1,2 and Robert E. Kingston1,2,*

Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 021141; Department of Genetics, Harvard Medical School, Boston, Massachusetts 021152; and Graduate Program, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 465563

Received 19 August 1998/Returned for modification 8 October 1998/Accepted 13 December 1998

The histone N-terminal tails have been shown previously to be important for chromatin assembly, remodeling, and stability. We have tested the ability of human SWI-SNF (hSWI-SNF) to remodel nucleosomes whose tails have been cleaved through a limited trypsin digestion. We show that hSWI-SNF is able to remodel tailless mononucleosomes and nucleosomal arrays, although hSWI-SNF remodeling of tailless nucleosomes is less effective than remodeling of nucleosomes with tails. Analogous to previous observations with tailed nucleosomal templates, we show both (i) that hSWI-SNF-remodeled trypsinized mononucleosomes and arrays are stable for 30 min in the remodeled conformation after removal of ATP and (ii) that the remodeled tailless mononucleosome can be isolated on a nondenaturing acrylamide gel as a novel species. Thus, nucleosome remodeling by hSWI-SNF can occur via interactions with a tailless nucleosome core.

* Corresponding author. Mailing address: Department of Molecular Biology, Wellman 10, Massachusetts General Hospital, Boston, MA 02114. Phone: (617) 726-5990. Fax: (617) 726-5949. E-mail: kingston{at}frodo.mgh.harvard.edu.

dagger This paper is dedicated to Veronica Blasquez in remembrance of her guidance, encouragement, and friendship.

Molecular and Cellular Biology, March 1999, p. 2088-2097, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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