This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muldrow, T. A.
Right arrow Articles by Auble, D. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muldrow, T. A.
Right arrow Articles by Auble, D. T.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 1999, p. 2835-2845, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

MOT1 Can Activate Basal Transcription In Vitro by Regulating the Distribution of TATA Binding Protein between Promoter and Nonpromoter Sites

Tamara A. Muldrow,1 Allyson M. Campbell,2 P. Anthony Weil,2 and David T. Auble1,*

Department of Biochemistry and Molecular Genetics, University of Virginia Health Science Center, Charlottesville, Virginia 22908,1 and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-06152

Received 10 December 1998/Accepted 13 January 1999

MOT1 is an ATPase which can dissociate TATA binding protein (TBP)-DNA complexes in a reaction requiring ATP hydrolysis. Consistent with this observation, MOT1 can repress basal transcription in vitro. Paradoxically, however, some genes, such as HIS4, appear to require MOT1 as an activator of transcription in vivo. To further investigate the function of MOT1 in basal transcription, we performed in vitro transcription reactions using yeast nuclear extracts depleted of MOT1. Quantitation of MOT1 revealed that it is an abundant protein, with nuclear extracts from wild-type cells containing a molar excess of MOT1 over TBP. Surprisingly, MOT1 can weakly activate basal transcription in vitro. This activation by MOT1 is detectable with amounts of MOT1 that are approximately stoichiometric to TBP. With amounts of MOT1 similar to those present in wild-type nuclear extracts, MOT1 behaves as a weak repressor of basal transcription. These results suggest that MOT1 might activate transcription via an indirect mechanism in which limiting TBP can be liberated from nonpromoter sites for use at promoters. In support of this idea, excess nonpromoter DNA sequesters TBP and represses transcription, but this effect can be reversed by addition of MOT1. These results help to reconcile previous in vitro and in vivo results and expand the repertoire of transcriptional control strategies to include factor-assisted redistribution of TBP between promoter and nonpromoter sites.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Genetics, University of Virginia Health Science Center, Charlottesville, VA 22908. Phone: (804) 243-2629. Fax: (804) 924-5069. E-mail: dta4n{at}virginia.edu.

Molecular and Cellular Biology, April 1999, p. 2835-2845, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Takahashi, H., Martin-Brown, S., Washburn, M. P., Florens, L., Conaway, J. W., Conaway, R. C. (2009). Proteomics Reveals a Physical and Functional Link between Hepatocyte Nuclear Factor 4{alpha} and Transcription Factor IID. J. Biol. Chem. 284: 32405-32412 [Abstract] [Full Text]  
  • Sprouse, R. O., Wells, M. N., Auble, D. T. (2009). TATA-binding Protein Variants That Bypass the Requirement for Mot1 in Vivo. J. Biol. Chem. 284: 4525-4535 [Abstract] [Full Text]  
  • Arnett, D. R., Jennings, J. L., Tabb, D. L., Link, A. J., Weil, P. A. (2008). A Proteomics Analysis of Yeast Mot1p Protein-Protein Associations: Insights into Mechanism. Mol. Cell. Proteomics 7: 2090-2106 [Abstract] [Full Text]  
  • van Werven, F. J., van Bakel, H., van Teeffelen, H. A.A.M., Altelaar, A.F. M., Koerkamp, M. G., Heck, A. J.R., Holstege, F. C.P., Timmers, H.Th. M. (2008). Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome. Genes Dev. 22: 2359-2369 [Abstract] [Full Text]  
  • Dasgupta, A., Sprouse, R. O., French, S., Aprikian, P., Hontz, R., Juedes, S. A., Smith, J. S., Beyer, A. L., Auble, D. T. (2007). Regulation of rRNA Synthesis by TATA-Binding Protein-Associated Factor Mot1. Mol. Cell. Biol. 27: 2886-2896 [Abstract] [Full Text]  
  • Voronkova, V., Kacherovsky, N., Tachibana, C., Yu, D., Young, E. T. (2006). Snf1-Dependent and Snf1-Independent Pathways of Constitutive ADH2 Expression in Saccharomyces cerevisiae. Genetics 172: 2123-2138 [Abstract] [Full Text]  
  • Horwitz, A. A., Sankaran, S., Parvin, J. D. (2006). Direct Stimulation of Transcription Initiation by BRCA1 Requires Both Its Amino and Carboxyl Termini. J. Biol. Chem. 281: 8317-8320 [Abstract] [Full Text]  
  • Klejman, M. P., Pereira, L. A., van Zeeburg, H. J. T., Gilfillan, S., Meisterernst, M., Timmers, H. T. M. (2004). NC2{alpha} Interacts with BTAF1 and Stimulates Its ATP-Dependent Association with TATA-Binding Protein. Mol. Cell. Biol. 24: 10072-10082 [Abstract] [Full Text]  
  • Pereira, L. A., Klejman, M. P., Ruhlmann, C., Kavelaars, F., Oulad-Abdelghani, M., Timmers, H. Th. M., Schultz, P. (2004). Molecular Architecture of the Basal Transcription Factor B-TFIID. J. Biol. Chem. 279: 21802-21807 [Abstract] [Full Text]  
  • Borkovich, K. A., Alex, L. A., Yarden, O., Freitag, M., Turner, G. E., Read, N. D., Seiler, S., Bell-Pedersen, D., Paietta, J., Plesofsky, N., Plamann, M., Goodrich-Tanrikulu, M., Schulte, U., Mannhaupt, G., Nargang, F. E., Radford, A., Selitrennikoff, C., Galagan, J. E., Dunlap, J. C., Loros, J. J., Catcheside, D., Inoue, H., Aramayo, R., Polymenis, M., Selker, E. U., Sachs, M. S., Marzluf, G. A., Paulsen, I., Davis, R., Ebbole, D. J., Zelter, A., Kalkman, E. R., O'Rourke, R., Bowring, F., Yeadon, J., Ishii, C., Suzuki, K., Sakai, W., Pratt, R. (2004). Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism. Microbiol. Mol. Biol. Rev. 68: 1-108 [Abstract] [Full Text]  
  • Kou, H., Irvin, J. D., Huisinga, K. L., Mitra, M., Pugh, B. F. (2003). Structural and Functional Analysis of Mutations along the Crystallographic Dimer Interface of the Yeast TATA Binding Protein. Mol. Cell. Biol. 23: 3186-3201 [Abstract] [Full Text]  
  • Estruch, F., Cole, C. N. (2003). An Early Function during Transcription for the Yeast mRNA Export Factor Dbp5p/Rat8p Suggested by Its Genetic and Physical Interactions with Transcription Factor IIH Components. Mol. Biol. Cell 14: 1664-1676 [Abstract] [Full Text]  
  • Geisberg, J. V., Moqtaderi, Z., Kuras, L., Struhl, K. (2002). Mot1 Associates with Transcriptionally Active Promoters and Inhibits Association of NC2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 8122-8134 [Abstract] [Full Text]  
  • Dasgupta, A., Darst, R. P., Martin, K. J., Afshari, C. A., Auble, D. T. (2002). Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms. Proc. Natl. Acad. Sci. USA 99: 2666-2671 [Abstract] [Full Text]  
  • Song, W., Solimeo, H., Rupert, R. A., Yadav, N. S., Zhu, Q. (2002). Functional Dissection of a Rice Dr1/DrAp1 Transcriptional Repression Complex. Plant Cell 14: 181-195 [Abstract] [Full Text]  
  • Pereira, L. A., van der Knaap, J. A., van den Boom, V., van den Heuvel, F. A. J., Timmers, H. T. M. (2001). TAFII170 Interacts with the Concave Surface of TATA-Binding Protein To Inhibit Its DNA Binding Activity. Mol. Cell. Biol. 21: 7523-7534 [Abstract] [Full Text]  
  • Neuwald, A. F., Hirano, T. (2000). HEAT Repeats Associated with Condensins, Cohesins, and Other Complexes Involved in Chromosome-Related Functions. Genome Res 10: 1445-1452 [Abstract] [Full Text]  
  • Crowe, A. J., Sang, L., Li, K. K., Lee, K. C., Spear, B. T., Barton, M. C. (1999). Hepatocyte Nuclear Factor 3 Relieves Chromatin-mediated Repression of the alpha -Fetoprotein Gene. J. Biol. Chem. 274: 25113-25120 [Abstract] [Full Text]  
  • Kobayashi, A., Miyake, T., Ohyama, Y., Kawaichi, M., Kokubo, T. (2001). Mutations in the TATA-binding Protein, Affecting Transcriptional Activation, Show Synthetic Lethality with the TAF145 Gene Lacking the TAF N-terminal Domain in Saccharomyces cerevisiae. J. Biol. Chem. 276: 395-405 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»