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Molecular and Cellular Biology, April 1999, p. 3125-3135, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Concerted Activity of Tyrosine Phosphatase SHP-2 and Focal Adhesion Kinase in Regulation of Cell Motility

Santos Mañes,1,* Emilia Mira,1 Concepción Gómez-Mouton,1 Zhizuang Joe Zhao,2 Rosa Ana Lacalle,1 and Carlos Martínez-A.1

Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas,Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049 Madrid, Spain,1 and Hematology/Oncology Division, Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232-63052

Received 30 September 1998/Returned for modification 23 October 1998/Accepted 11 January 1999

The coordinated interplay of substrate adhesion and deadhesion is necessary for cell motility. Using MCF-7 cells, we found that insulin-like growth factor I (IGF-I) induces the adhesion of MCF-7 to vitronectin and collagen in a dose- and time-dependent manner, suggesting that IGF-I triggers the activation of different integrins. On the other hand, IGF-I promotes the association of insulin receptor substrate 1 with the focal adhesion kinase (FAK), paxillin, and the tyrosine phosphatase SHP-2, resulting in FAK and paxillin dephosphorylation. Abrogation of SHP-2 catalytic activity with a dominant-negative mutant (SHP2-C>S) abolishes IGF-I-induced FAK dephosphorylation, and cells expressing SHP2-C>S show reduced IGF-I-stimulated chemotaxis compared with either mock- or SHP-2 wild-type-transfected cells. This impairment of cell migration is recovered by reintroduction of a catalytically active SHP-2. Interestingly, SHP-2-C>S cells show a larger number of focal adhesion contacts than wild-type cells, suggesting that SHP-2 activity participates in the integrin deactivation process. Although SHP-2 regulates mitogen-activated protein kinase activity, the mitogen-activated protein kinase kinase inhibitor PD-98059 has only a marginal effect on MCF-7 cell migration. The role of SHP-2 as a general regulator of cell chemotaxis induced by other chemotactic agents and integrins is discussed.

* Corresponding author. Mailing address: Department of Immunology and Oncology, Centro Nacional de Biotecnología, CSIC, Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049 Madrid, Spain. Phone: (34) 91/585-4660. Fax: (34) 91/372-0493. E-mail: smanes{at}cnb.uam.es.

Molecular and Cellular Biology, April 1999, p. 3125-3135, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Shamri, R., Grabovsky, V., Feigelson, S. W., Dwir, O., van Kooyk, Y., Alon, R. (2002). Chemokine Stimulation of Lymphocyte alpha 4 Integrin Avidity but Not of Leukocyte Function-associated Antigen-1 Avidity to Endothelial Ligands under Shear Flow Requires Cholesterol Membrane Rafts. J. Biol. Chem. 277: 40027-40035 [Abstract] [Full Text]  
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  • Abbi, S., Ueda, H., Zheng, C., Cooper, L. A., Zhao, J., Christopher, R., Guan, J.-L. (2002). Regulation of Focal Adhesion Kinase by a Novel Protein Inhibitor FIP200. Mol. Biol. Cell 13: 3178-3191 [Abstract] [Full Text]  
  • Wilcox-Adelman, S. A., Denhez, F., Goetinck, P. F. (2002). Syndecan-4 Modulates Focal Adhesion Kinase Phosphorylation. J. Biol. Chem. 277: 32970-32977 [Abstract] [Full Text]  
  • Amieux, P. S., Howe, D. G., Knickerbocker, H., Lee, D. C., Su, T., Laszlo, G. S., Idzerda, R. L., McKnight, G. S. (2002). Increased Basal cAMP-dependent Protein Kinase Activity Inhibits the Formation of Mesoderm-derived Structures in the Developing Mouse Embryo. J. Biol. Chem. 277: 27294-27304 [Abstract] [Full Text]  
  • Lacalle, R. A., Mira, E., Gomez-Mouton, C., Jimenez-Baranda, S., Martinez-A., C., Manes, S. (2002). Specific SHP-2 partitioning in raft domains triggers integrin-mediated signaling via Rho activation. JCB 157: 277-289 [Abstract] [Full Text]  
  • Buckley, D. A., Cheng, A., Kiely, P. A., Tremblay, M. L., O'Connor, R. (2002). Regulation of Insulin-Like Growth Factor Type I (IGF-I) Receptor Kinase Activity by Protein Tyrosine Phosphatase 1B (PTP-1B) and Enhanced IGF-I-Mediated Suppression of Apoptosis and Motility in PTP-1B-Deficient Fibroblasts. Mol. Cell. Biol. 22: 1998-2010 [Abstract] [Full Text]  
  • Ivaska, J., Nissinen, L., Immonen, N., Eriksson, J. E., Kahari, V.-M., Heino, J. (2002). Integrin {alpha}2{beta}1 Promotes Activation of Protein Phosphatase 2A and Dephosphorylation of Akt and Glycogen Synthase Kinase 3{beta}. Mol. Cell. Biol. 22: 1352-1359 [Abstract] [Full Text]  
  • Smith, R. K., Carroll, P. M., Allard, J. D., Simon, M. A. (2002). MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila receptor tyrosine kinase signaling. Development 129: 71-82 [Abstract] [Full Text]  
  • Hu, Y., Szente, B., Kiely, J.-M., Gimbrone, M. A. Jr. (2001). Molecular Events in Transmembrane Signaling via E-selectin. SHP2 ASSOCIATION, ADAPTOR PROTEIN COMPLEX FORMATION AND ERK1/2 ACTIVATION. J. Biol. Chem. 276: 48549-48553 [Abstract] [Full Text]  
  • Brown, C., Lin, Y., Hassid, A. (2001). Requirement of protein tyrosine phosphatase SHP2 for NO-stimulated vascular smooth muscle cell motility. Am. J. Physiol. Heart Circ. Physiol. 281: H1598-H1605 [Abstract] [Full Text]  
  • Lu, Z., Jiang, G., Blume-Jensen, P., Hunter, T. (2001). Epidermal Growth Factor-Induced Tumor Cell Invasion and Metastasis Initiated by Dephosphorylation and Downregulation of Focal Adhesion Kinase. Mol. Cell. Biol. 21: 4016-4031 [Abstract] [Full Text]  
  • Ikeo, S., Yamauchi, K., Shigematsu, S., Nakajima, K., Aizawa, T., Hashizume, K. (2001). Differential effects of growth hormone and insulin-like growth factor I on human endothelial cell migration. Am. J. Physiol. Cell Physiol. 280: C1255-C1261 [Abstract] [Full Text]  
  • BROCKMANN, G. A., HALEY, C. S., WOLF, E., KARLE, S., KRATZSCH, J., RENNE, U., SCHWERIN, M., HOEFLICH, A. (2001). Genome-wide search for loci controlling serum IGF binding protein levels of mice. FASEB J. 15: 978-987 [Abstract] [Full Text]  
  • Chernock, R. D., Cherla, R. P., Ganju, R. K. (2001). SHP2 and cbl participate in {alpha}-chemokine receptor CXCR4-mediated signaling pathways. Blood 97: 608-615 [Abstract] [Full Text]  
  • Klingbeil, C. K., Hauck, C. R., Hsia, D. A., Jones, K.C., Reider, S. R., Schlaepfer, D. D. (2001). Targeting Pyk2 to {beta}1-Integrin-Containing Focal Contacts Rescues Fibronectin-Stimulated Signaling and Haptotactic Motility Defects of Focal Adhesion Kinase-Null Cells. JCB 152: 97-110 [Abstract] [Full Text]  
  • Maile, L., Badley-Clarke, J, Clemmons, D. (2001). Structural analysis of the role of the (&bgr;)3 subunit of the (&agr;)V(&bgr;)3 integrin in IGF-I signaling. J. Cell Sci. 114: 1417-1425 [Abstract]  
  • Kruger, J., Butler, J. R., Cherapanov, V., Dong, Q., Ginzberg, H., Govindarajan, A., Grinstein, S., Siminovitch, K. A., Downey, G. P. (2000). Deficiency of Src Homology 2-Containing Phosphatase 1 Results in Abnormalities in Murine Neutrophil Function: Studies in Motheaten Mice. J. Immunol. 165: 5847-5859 [Abstract] [Full Text]  
  • Kodama, A., Matozaki, T., Fukuhara, A., Kikyo, M., Ichihashi, M., Takai, Y. (2000). Involvement of an SHP-2-Rho Small G Protein Pathway in Hepatocyte Growth Factor/Scatter Factor-induced Cell Scattering. Mol. Biol. Cell 11: 2565-2575 [Abstract] [Full Text]  
  • Sheibani, N., Sorenson, C. M., Frazier, W. A. (2000). Differential Modulation of Cadherin-mediated Cell-Cell Adhesion by Platelet Endothelial Cell Adhesion Molecule-1 Isoforms through Activation of Extracellular Regulated Kinases. Mol. Biol. Cell 11: 2793-2802 [Abstract] [Full Text]  
  • Monno, S., Newman, M. V., Cook, M., Lowe, W. L. Jr. (2000). Insulin-Like Growth Factor I Activates c-Jun N-Terminal Kinase in MCF-7 Breast Cancer Cells. Endocrinology 141: 544-550 [Abstract] [Full Text]  
  • Petrone, A, Sap, J (2000). Emerging issues in receptor protein tyrosine phosphatase function: lifting fog or simply shifting?. J. Cell Sci. 113: 2345-2354 [Abstract]  
  • O'Reilly, A. M., Pluskey, S., Shoelson, S. E., Neel, B. G. (2000). Activated Mutants of SHP-2 Preferentially Induce Elongation of Xenopus Animal Caps. Mol. Cell. Biol. 20: 299-311 [Abstract] [Full Text]  
  • Higashi, H., Tsutsumi, R., Muto, S., Sugiyama, T., Azuma, T., Asaka, M., Hatakeyama, M. (2002). SHP-2 Tyrosine Phosphatase as an Intracellular Target of Helicobacter pylori CagA Protein. Science 295: 683-686 [Abstract] [Full Text]  
  • Pluskota, E., Chen, Y., D'Souza, S. E. (2000). Src Homology Domain 2-containing Tyrosine Phosphatase 2 Associates with Intercellular Adhesion Molecule 1 to Regulate Cell Survival. J. Biol. Chem. 275: 30029-30036 [Abstract] [Full Text]  
  • Escalante, M., Courtney, J., Chin, W. G., Teng, K. K., Kim, J.-I., Fajardo, J. E., Mayer, B. J., Hempstead, B. L., Birge, R. B. (2000). Phosphorylation of c-Crk II on the Negative Regulatory Tyr222 Mediates Nerve Growth Factor-induced Cell Spreading and Morphogenesis. J. Biol. Chem. 275: 24787-24797 [Abstract] [Full Text]  
  • Hauck, C. R., Hsia, D. A., Schlaepfer, D. D. (2000). Focal Adhesion Kinase Facilitates Platelet-derived Growth Factor-BB-stimulated ERK2 Activation Required for Chemotaxis Migration of Vascular Smooth Muscle Cells. J. Biol. Chem. 275: 41092-41099 [Abstract] [Full Text]  
  • Xu, F., Zhao, R., Peng, Y., Guerrah, A., Zhao, Z. J. (2001). Association of Tyrosine Phosphatase SHP-2 with F-actin at Low Cell Densities. J. Biol. Chem. 276: 29479-29484 [Abstract] [Full Text]  
  • Ligensa, T., Krauss, S., Demuth, D., Schumacher, R., Camonis, J., Jaques, G., Weidner, K. M. (2001). A PDZ Domain Protein Interacts with the C-terminal Tail of the Insulin-like Growth Factor-1 Receptor but Not with the Insulin Receptor. J. Biol. Chem. 276: 33419-33427 [Abstract] [Full Text]  
  • Derkinderen, P., Toutant, M., Kadare, G., Ledent, C., Parmentier, M., Girault, J.-A. (2001). Dual Role of Fyn in the Regulation of FAK+6,7 by Cannabinoids in Hippocampus. J. Biol. Chem. 276: 38289-38296 [Abstract] [Full Text]  
  • Playford, M. P., Bicknell, D., Bodmer, W. F., Macaulay, V. M. (2000). Insulin-like growth factor 1 regulates the location, stability, and transcriptional activity of beta -catenin. Proc. Natl. Acad. Sci. USA 97: 12103-12108 [Abstract] [Full Text]  
  • Lacalle, R. A., Mira, E., Gomez-Mouton, C., Jimenez-Baranda, S., Martinez-A., C., Manes, S. (2002). Specific SHP-2 partitioning in raft domains triggers integrin-mediated signaling via Rho activation. JCB 157: 277-289 [Abstract] [Full Text]  
  • Sorenson, C. M., Sheibani, N. (2002). Altered regulation of SHP-2 and PTP 1B tyrosine phosphatases in cystic kidneys from bcl-2 -/- mice. Am. J. Physiol. Renal Physiol. 282: F442-F450 [Abstract] [Full Text]  


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