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Molecular and Cellular Biology, July 1999, p. 4832-4842, Vol. 19, No. 7
0270-7306/99/$04.00+0

Use of a Recombination Reporter Insert To Define Meiotic Recombination Domains on Chromosome III of Saccharomyces cerevisiae

Valérie Borde, Tzu-Chen Wu, and Michael Lichten*

Laboratory of Biochemistry, Division of Basic Science, National Cancer Institute, Bethesda, Maryland 20892

Received 10 February 1999/Returned for modification 29 March 1999/Accepted 9 April 1999

In Saccharomyces cerevisiae, meiotic recombination is initiated by DNA double-strand breaks (DSBs). DSBs usually occur in intergenic regions that display nuclease hypersensitivity in digests of chromatin. DSBs are distributed nonuniformly across chromosomes; on chromosome III, DSBs are concentrated in two "hot" regions, one in each chromosome arm. DSBs occur rarely in regions within about 40 kb of each telomere and in an 80-kb region in the center of the chromosome, just to the right of the centromere. We used recombination reporter inserts containing arg4 mutant alleles to show that the "cold" properties of the central DSB-deficient region are imposed on DNA inserted in the region. Cold region inserts display DSB and recombination frequencies that are substantially less than those seen with similar inserts in flanking hot regions. This occurs without apparent change in chromatin structure, as the same pattern and level of DNase I hypersensitivity is seen in chromatin of hot and cold region inserts. These data are consistent with the suggestion that features of higher-order chromosome structure or chromosome dynamics act in a target sequence-independent manner to control where recombination events initiate during meiosis.

* Corresponding author. Mailing address: Bldg. 37 Room 4C03, NIH, 37 Convent Dr. MSC 4255, Bethesda, MD 20892-4255. Phone: (301) 496-3393. Fax: (301) 402-3095. E-mail: lichten{at}helix.nih.gov.

Molecular and Cellular Biology, July 1999, p. 4832-4842, Vol. 19, No. 7
0270-7306/99/$04.00+0


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