This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hsieh, Y.-J.
Right arrow Articles by Roeder, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsieh, Y.-J.
Right arrow Articles by Roeder, R. G.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 1999, p. 4944-4952, Vol. 19, No. 7
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Characterization of Two Evolutionarily Conserved Subunits (TFIIIC102 and TFIIIC63) of Human TFIIIC and Their Involvement in Functional Interactions with TFIIIB and RNA Polymerase III

Yng-Ju Hsieh, Zhengxin Wang, Robert Kovelman,dagger and Robert G. Roeder*

Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10021

Received 9 March 1999/Returned for modification 15 April 1999/Accepted 26 April 1999

Human transcription factor IIIC (hTFIIIC) is a multisubunit complex that mediates transcription of class III genes through direct recognition of promoters (for tRNA and virus-associated RNA genes) or promoter-TFIIIA complexes (for the 5S RNA gene) and subsequent recruitment of TFIIIB and RNA polymerase III. We describe the cognate cDNA cloning and characterization of two subunits (hTFIIIC63 and hTFIIIC102) that are present within a DNA-binding subcomplex (TFIIIC2) of TFIIIC and are related in structure and function to two yeast TFIIIC subunits (yTFIIIC95 and yTFIIIC131) previously shown to interact, respectively, with the promoter (A box) and with a subunit of yeast TFIIIB. hTFIIIC63 and hTFIIIC102 show parallel in vitro interactions with the homologous human TFIIIB and RNA polymerase III components, as well as additional interactions that may facilitate both TFIIIB and RNA polymerase III recruitment. These include novel interactions of hTFIIIC63 with hTFIIIC102, with hTFIIIB90, and with hRPC62, in addition to the hTFIIIC102-hTFIIIB90 and hTFIIIB90-hRPC39 interactions that parallel the previously described interactions in yeast. As reported for yTFIIIC131, hTFIIIC102 contains acidic and basic regions, tetratricopeptide repeats (TPRs), and a helix-loop-helix domain, and mutagenesis studies have implicated the TPRs in interactions both with hTFIIIC63 and with hTFIIIB90. These observations further document conservation from yeast to human of the structure and function of the RNA polymerase III transcription machinery, but in addition, they provide new insights into the function of hTFIIIC and suggest direct involvement in recruitment of both TFIIIB and RNA polymerase III.

* Corresponding author. Mailing address: Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021. Phone: (212) 327-7600. Fax: (212) 327-7949. E-mail: roeder{at}rockvax.rockefeller.edu.

dagger Present address: The Scripps Research Institute, La Jolla, CA 92037.

Molecular and Cellular Biology, July 1999, p. 4944-4952, Vol. 19, No. 7
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hufton, A. L., Mathia, S., Braun, H., Georgi, U., Lehrach, H., Vingron, M., Poustka, A. J., Panopoulou, G. (2009). Deeply conserved chordate noncoding sequences preserve genome synteny but do not drive gene duplicate retention. Genome Res 19: 2036-2051 [Abstract] [Full Text]  
  • Mertens, C., Roeder, R. G. (2008). Different Functional Modes of p300 in Activation of RNA Polymerase III Transcription from Chromatin Templates. Mol. Cell. Biol. 28: 5764-5776 [Abstract] [Full Text]  
  • Dumay-Odelot, H., Marck, C., Durrieu-Gaillard, S., Lefebvre, O., Jourdain, S., Prochazkova, M., Pflieger, A., Teichmann, M. (2007). Identification, Molecular Cloning, and Characterization of the Sixth Subunit of Human Transcription Factor TFIIIC. J. Biol. Chem. 282: 17179-17189 [Abstract] [Full Text]  
  • Lin, Y.-H., Friederichs, J., Black, M. A., Mages, J., Rosenberg, R., Guilford, P. J., Phillips, V., Thompson-Fawcett, M., Kasabov, N., Toro, T., Merrie, A. E., van Rij, A., Yoon, H.-S., McCall, J. L., Siewert, J. R., Holzmann, B., Reeve, A. E. (2007). Multiple Gene Expression Classifiers from Different Array Platforms Predict Poor Prognosis of Colorectal Cancer. Clin. Cancer Res. 13: 498-507 [Abstract] [Full Text]  
  • Innes, F., Ramsbottom, B., White, R. J. (2006). A test of the model that RNA polymerase III transcription is regulated by selective induction of the 110 kDa subunit of TFIIIC. Nucleic Acids Res 34: 3399-3407 [Abstract] [Full Text]  
  • Ferrari, R., Rivetti, C., Acker, J., Dieci, G. (2004). Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiation. Proc. Natl. Acad. Sci. USA 101: 13442-13447 [Abstract] [Full Text]  
  • Liao, Y., Willis, I. M., Moir, R. D. (2003). The Brf1 and Bdp1 Subunits of Transcription Factor TFIIIB Bind to Overlapping Sites in the Tetratricopeptide Repeats of Tfc4. J. Biol. Chem. 278: 44467-44474 [Abstract] [Full Text]  
  • Weser, S., Riemann, J., Seifart, K. H., Meissner, W. (2003). Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene. Nucleic Acids Res 31: 2408-2416 [Abstract] [Full Text]  
  • Jourdain, S., Acker, J., Ducrot, C., Sentenac, A., Lefebvre, O. (2003). The tau 95 Subunit of Yeast TFIIIC Influences Upstream and Downstream Functions of TFIIIC{middle dot}DNA Complexes. J. Biol. Chem. 278: 10450-10457 [Abstract] [Full Text]  
  • Felton-Edkins, Z. A., White, R. J. (2002). Multiple Mechanisms Contribute to the Activation of RNA Polymerase III Transcription in Cells Transformed by Papovaviruses. J. Biol. Chem. 277: 48182-48191 [Abstract] [Full Text]  
  • Hu, P., Wu, S., Sun, Y., Yuan, C.-C., Kobayashi, R., Myers, M. P., Hernandez, N. (2002). Characterization of Human RNA Polymerase III Identifies Orthologues for Saccharomyces cerevisiae RNA Polymerase III Subunits. Mol. Cell. Biol. 22: 8044-8055 [Abstract] [Full Text]  
  • Schramm, L., Hernandez, N. (2002). Recruitment of RNA polymerase III to its target promoters. Genes Dev. 16: 2593-2620 [Full Text]  
  • Murphy, C., Wang, Z., Roeder, R. G., Gall, J. G. (2002). RNA Polymerase III in Cajal Bodies and Lampbrush Chromosomes of the Xenopus Oocyte Nucleus. Mol. Biol. Cell 13: 3466-3476 [Abstract] [Full Text]  
  • Sabri, N., Farrants, A.-K. O., Hellman, U., Visa, N. (2002). Evidence for a Posttranscriptional Role of a TFIIICalpha -like Protein in Chironomus tentans. Mol. Biol. Cell 13: 1765-1777 [Abstract] [Full Text]  
  • Moir, R. D., Puglia, K. V., Willis, I. M. (2002). Autoinhibition of TFIIIB70 Binding by the Tetratricopeptide Repeat-containing Subunit of TFIIIC. J. Biol. Chem. 277: 694-701 [Abstract] [Full Text]  
  • Dumay-Odelot, H., Acker, J., Arrebola, R., Sentenac, A., Marck, C. (2002). Multiple Roles of the {tau}131 Subunit of Yeast Transcription Factor IIIC (TFIIIC) in TFIIIB Assembly. Mol. Cell. Biol. 22: 298-308 [Abstract] [Full Text]  
  • Aye, M., Dildine, S. L., Claypool, J. A., Jourdain, S., Sandmeyer, S. B. (2001). A Truncation Mutant of the 95-Kilodalton Subunit of Transcription Factor IIIC Reveals Asymmetry in Ty3 Integration. Mol. Cell. Biol. 21: 7839-7851 [Abstract] [Full Text]  
  • Hamada, M., Huang, Y., Lowe, T. M., Maraia, R. J. (2001). Widespread Use of TATA Elements in the Core Promoters for RNA Polymerases III, II, and I in Fission Yeast. Mol. Cell. Biol. 21: 6870-6881 [Abstract] [Full Text]  
  • Pluta, K., Lefebvre, O., Martin, N. C., Smagowicz, W. J., Stanford, D. R., Ellis, S. R., Hopper, A. K., Sentenac, A., Boguta, M. (2001). Maf1p, a Negative Effector of RNA Polymerase III in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 5031-5040 [Abstract] [Full Text]  
  • Huang, Y., Maraia, R. J. (2001). Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human. Nucleic Acids Res 29: 2675-2690 [Abstract] [Full Text]  
  • Li, T.-H., Kim, C., Rubin, C. M., Schmid, C. W. (2000). K562 cells implicate increased chromatin accessibility in Alu transcriptional activation. Nucleic Acids Res 28: 3031-3039 [Abstract] [Full Text]  
  • Paule, M. R., White, R. J. (2000). SURVEY AND SUMMARY Transcription by RNA polymerases I and III. Nucleic Acids Res 28: 1283-1298 [Abstract] [Full Text]  
  • Deprez, E., Arrebola, R., Conesa, C., Sentenac, A. (1999). A Subunit of Yeast TFIIIC Participates in the Recruitment of TATA-Binding Protein. Mol. Cell. Biol. 19: 8042-8051 [Abstract] [Full Text]  
  • Dumay, H., Rubbi, L., Sentenac, A., Marck, C. (1999). Interaction between Yeast RNA Polymerase III and Transcription Factor TFIIIC via ABC10alpha and tau 131 Subunits. J. Biol. Chem. 274: 33462-33468 [Abstract] [Full Text]  
  • Hsieh, Y.-J., Kundu, T. K., Wang, Z., Kovelman, R., Roeder, R. G. (1999). The TFIIIC90 Subunit of TFIIIC Interacts with Multiple Components of the RNA Polymerase III Machinery and Contains a Histone-Specific Acetyltransferase Activity. Mol. Cell. Biol. 19: 7697-7704 [Abstract] [Full Text]  
  • Hamada, M., Sakulich, A. L., Koduru, S. B., Maraia, R. J. (2000). Transcription Termination by RNA Polymerase III in Fission Yeast. A GENETIC AND BIOCHEMICALLY TRACTABLE MODEL SYSTEM. J. Biol. Chem. 275: 29076-29081 [Abstract] [Full Text]  
  • Moir, R. D., Puglia, K. V., Willis, I. M. (2000). Interactions between the Tetratricopeptide Repeat-containing Transcription Factor TFIIIC131 and Its Ligand, TFIIIB70. EVIDENCE FOR A CONFORMATIONAL CHANGE IN THE COMPLEX. J. Biol. Chem. 275: 26591-26598 [Abstract] [Full Text]  
  • Huang, Y., Hamada, M., Maraia, R. J. (2000). Isolation and Cloning of Four Subunits of a Fission Yeast TFIIIC Complex That Includes an Ortholog of the Human Regulatory Protein TFIIICbeta. J. Biol. Chem. 275: 31480-31487 [Abstract] [Full Text]  
  • Chong, S. S., Hu, P., Hernandez, N. (2001). Reconstitution of Transcription from the Human U6 Small Nuclear RNA Promoter with Eight Recombinant Polypeptides and a Partially Purified RNA Polymerase III Complex. J. Biol. Chem. 276: 20727-20734 [Abstract] [Full Text]  
  • Mertens, C., Hofmann, I., Wang, Z., Teichmann, M., Chong, S. S., Schnolzer, M., Franke, W. W. (2001). Nuclear particles containing RNA polymerase III complexes associated with the junctional plaque protein plakophilin 2. Proc. Natl. Acad. Sci. USA 98: 7795-7800 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»