This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Endoh, H.
Right arrow Articles by Kato, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Endoh, H.
Right arrow Articles by Kato, S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 1999, p. 5363-5372, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor alpha

Hideki Endoh,1,2 Kazunori Maruyama,1 Yoshikazu Masuhiro,2 Yoko Kobayashi,2 Masahide Goto,1 Hitoshi Tai,2 Junn Yanagisawa,2 Daniel Metzger,3 Seiichi Hashimoto,1 and Shigeaki Kato2,4,*

Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical, Tsukuba, Ibaraki 305-8585,1 Institute for Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032,2 and CREST, Japan Science and Technology, Kawaguchi, Saitama 332-0012,4 Japan, and Institut de Genetique et de Biologie Moleculaire et Cellulaire, (IGBMC)/CNRS/INSERM/ULP College de France, 67404 Illkirch Cedex, C.U. de Strasbourg, France3

Received 19 January 1999/Returned for modification 1 March 1999/Accepted 5 May 1999

The estrogen receptor (ER) regulates the expression of target genes in a ligand-dependent manner. The ligand-dependent activation function AF-2 of the ER is located in the ligand binding domain (LBD), while the N-terminal A/B domain (AF-1) functions in a ligand-independent manner when isolated from the LBD. AF-1 and AF-2 exhibit cell type and promoter context specificity. Furthermore, the AF-1 activity of the human ERalpha (hERalpha ) is enhanced through phosphorylation of the Ser118 residue by mitogen-activated protein kinase (MAPK). From MCF-7 cells, we purified and cloned a 68-kDa protein (p68) which interacted with the A/B domain but not with the LBD of hERalpha . Phosphorylation of hERalpha Ser118 potentiated the interaction with p68. We demonstrate that p68 enhanced the activity of AF-1 but not AF-2 and the estrogen-induced as well as the anti-estrogen-induced transcriptional activity of the full-length ERalpha in a cell-type-specific manner. However, it did not potentiate AF-1 or AF-2 of ERbeta , androgen receptor, retinoic acid receptor alpha, or mineralocorticoid receptor. We also show that the RNA helicase activity previously ascribed to p68 is dispensable for the ERalpha AF-1 coactivator activity and that p68 binds to CBP in vitro. Furthermore, the interaction region for p68 in the ERalpha A/B domain was essential for the full activity of hERalpha AF-1. Taken together, these findings show that p68 acts as a coactivator specific for the ERalpha AF-1 and strongly suggest that the interaction between p68 and the hERalpha A/B domain is regulated by MAPK-induced phosphorylation of Ser118.

* Corresponding author. Mailing address: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan. Phone: 81-3-5841-8478. Fax: 81-3-5841-8477. E-mail: uskato{at}hongo.ecc.u-tokyo.ac.jp.

Molecular and Cellular Biology, August 1999, p. 5363-5372, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lee, S. J., Chae, C., Wang, M. M. (2009). p150/Glued Modifies Nuclear Estrogen Receptor Function. Mol. Endocrinol. 23: 620-629 [Abstract] [Full Text]  
  • Gineste, R., Sirvent, A., Paumelle, R., Helleboid, S., Aquilina, A., Darteil, R., Hum, D. W., Fruchart, J.-C., Staels, B. (2008). Phosphorylation of Farnesoid X Receptor by Protein Kinase C Promotes Its Transcriptional Activity. Mol. Endocrinol. 22: 2433-2447 [Abstract] [Full Text]  
  • Clark, E. L., Coulson, A., Dalgliesh, C., Rajan, P., Nicol, S. M., Fleming, S., Heer, R., Gaughan, L., Leung, H. Y., Elliott, D. J., Fuller-Pace, F. V., Robson, C. N. (2008). The RNA Helicase p68 Is a Novel Androgen Receptor Coactivator Involved in Splicing and Is Overexpressed in Prostate Cancer. Cancer Res. 68: 7938-7946 [Abstract] [Full Text]  
  • Chen, W., Dang, T., Blind, R. D., Wang, Z., Cavasotto, C. N., Hittelman, A. B., Rogatsky, I., Logan, S. K., Garabedian, M. J. (2008). Glucocorticoid Receptor Phosphorylation Differentially Affects Target Gene Expression. Mol. Endocrinol. 22: 1754-1766 [Abstract] [Full Text]  
  • Hawse, J. R., Subramaniam, M., Monroe, D. G., Hemmingsen, A. H., Ingle, J. N., Khosla, S., Oursler, M. J., Spelsberg, T. C. (2008). Estrogen Receptor {beta} Isoform-Specific Induction of Transforming Growth Factor {beta}-Inducible Early Gene-1 in Human Osteoblast Cells: An Essential Role for the Activation Function 1 Domain. Mol. Endocrinol. 22: 1579-1595 [Abstract] [Full Text]  
  • Vucetic, Z., Zhang, Z., Zhao, J., Wang, F., Soprano, K. J., Soprano, D. R. (2008). Acinus-S' Represses Retinoic Acid Receptor (RAR)-Regulated Gene Expression through Interaction with the B Domains of RARs. Mol. Cell. Biol. 28: 2549-2558 [Abstract] [Full Text]  
  • Picard, N., Charbonneau, C., Sanchez, M., Licznar, A., Busson, M., Lazennec, G., Tremblay, A. (2008). Phosphorylation of Activation Function-1 Regulates Proteasome-Dependent Nuclear Mobility and E6-Associated Protein Ubiquitin Ligase Recruitment to the Estrogen Receptor {beta}. Mol. Endocrinol. 22: 317-330 [Abstract] [Full Text]  
  • Green, C. D., Thompson, P. D., Johnston, P. G., El-Tanani, M. K. (2007). Interaction between Transcription Factor, Basal Transcription Factor 3, and the NH2-Terminal Domain of Human Estrogen Receptor {alpha}. Mol Cancer Res 5: 1191-1200 [Abstract] [Full Text]  
  • Sridharan, R., Smale, S. T. (2007). Predominant Interaction of Both Ikaros and Helios with the NuRD Complex in Immature Thymocytes. J. Biol. Chem. 282: 30227-30238 [Abstract] [Full Text]  
  • Han, W-D, Zhao, Y-L, Meng, Y-G, Zang, L, Wu, Z-Q, Li, Q, Si, Y-L, Huang, K, Ba, J-M, Morinaga, H, Nomura, M, Mu, Y-M (2007). Estrogenically regulated LRP16 interacts with estrogen receptor {alpha} and enhances the receptor's transcriptional activity. Endocr Relat Cancer 14: 741-753 [Abstract] [Full Text]  
  • Gallais, R., Demay, F., Barath, P., Finot, L., Jurkowska, R., Le Guevel, R., Gay, F., Jeltsch, A., Metivier, R., Salbert, G. (2007). Deoxyribonucleic Acid Methyl Transferases 3a and 3b Associate with the Nuclear Orphan Receptor COUP-TFI during Gene Activation. Mol. Endocrinol. 21: 2085-2098 [Abstract] [Full Text]  
  • Shin, S., Rossow, K. L., Grande, J. P., Janknecht, R. (2007). Involvement of RNA Helicases p68 and p72 in Colon Cancer. Cancer Res. 67: 7572-7578 [Abstract] [Full Text]  
  • Heldring, N., Pike, A., Andersson, S., Matthews, J., Cheng, G., Hartman, J., Tujague, M., Strom, A., Treuter, E., Warner, M., Gustafsson, J.-A. (2007). Estrogen Receptors: How Do They Signal and What Are Their Targets. Physiol. Rev. 87: 905-931 [Abstract] [Full Text]  
  • Yang, L., Lin, C., Zhao, S., Wang, H., Liu, Z.-R. (2007). Phosphorylation of p68 RNA Helicase Plays a Role in Platelet-derived Growth Factor-induced Cell Proliferation by Up-regulating Cyclin D1 and c-Myc Expression. J. Biol. Chem. 282: 16811-16819 [Abstract] [Full Text]  
  • Katzenellenbogen, R. A., Egelkrout, E. M., Vliet-Gregg, P., Gewin, L. C., Gafken, P. R., Galloway, D. A. (2007). NFX1-123 and Poly(A) Binding Proteins Synergistically Augment Activation of Telomerase in Human Papillomavirus Type 16 E6-Expressing Cells. J. Virol. 81: 3786-3796 [Abstract] [Full Text]  
  • Lopez-Garcia, J., Periyasamy, M., Thomas, R. S., Christian, M., Leao, M., Jat, P., Kindle, K. B., Heery, D. M., Parker, M. G., Buluwela, L., Kamalati, T., Ali, S. (2006). ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res 34: 6126-6136 [Abstract] [Full Text]  
  • Weitsman, G. E., Li, L., Skliris, G. P., Davie, J. R., Ung, K., Niu, Y., Curtis-Snell, L., Tomes, L., Watson, P. H., Murphy, L. C. (2006). Estrogen Receptor-{alpha} Phosphorylated at Ser118 Is Present at the Promoters of Estrogen-Regulated Genes and Is Not Altered Due to HER-2 Overexpression.. Cancer Res. 66: 10162-10170 [Abstract] [Full Text]  
  • Monroe, D. G., Secreto, F. J., Hawse, J. R., Subramaniam, M., Khosla, S., Spelsberg, T. C. (2006). Estrogen Receptor Isoform-specific Regulation of the Retinoblastoma-binding Protein 1 (RBBP1) Gene: ROLES OF AF1 AND ENHANCER ELEMENTS. J. Biol. Chem. 281: 28596-28604 [Abstract] [Full Text]  
  • Fuller-Pace, F. V. (2006). DExD/H box RNA helicases: multifunctional proteins with important roles in transcriptional regulation. Nucleic Acids Res 34: 4206-4215 [Abstract] [Full Text]  
  • Chao, C.-H., Chen, C.-M., Cheng, P.-L., Shih, J.-W., Tsou, A.-P., Wu Lee, Y.-H. (2006). DDX3, a DEAD Box RNA Helicase with Tumor Growth-Suppressive Property and Transcriptional Regulation Activity of the p21waf1/cip1 Promoter, Is a Candidate Tumor Suppressor.. Cancer Res. 66: 6579-6588 [Abstract] [Full Text]  
  • Ma, X.-H., Hu, S.-J., Ni, H., Zhao, Y.-C., Tian, Z., Liu, J.-L., Ren, G., Liang, X.-H., Yu, H., Wan, P., Yang, Z.-M. (2006). Serial Analysis of Gene Expression in Mouse Uterus at the Implantation Site. J. Biol. Chem. 281: 9351-9360 [Abstract] [Full Text]  
  • Zhang, H., Xie, X., Zhu, X., Zhu, J., Hao, C., Lu, Q., Ding, L., Liu, Y., Zhou, L., Liu, Y., Huang, C., Wen, C., Ye, Q. (2005). Stimulatory Cross-talk between NFAT3 and Estrogen Receptor in Breast Cancer Cells. J. Biol. Chem. 280: 43188-43197 [Abstract] [Full Text]  
  • Hall, J. M., McDonnell, D. P. (2005). Coregulators in Nuclear Estrogen Receptor Action: From Concept to Therapeutic Targeting. Mol. Interv. 5: 343-357 [Abstract] [Full Text]  
  • Leong, H., Sloan, J. R., Nash, P. D., Greene, G. L. (2005). Recruitment of Histone Deacetylase 4 to the N-Terminal Region of Estrogen Receptor {alpha}. Mol. Endocrinol. 19: 2930-2942 [Abstract] [Full Text]  
  • Ogawa, S., Oishi, H., Mezaki, Y., Kouzu-Fujita, M., Matsuyama, R., Nakagomi, M., Mori, E., Murayama, E., Nagasawa, H., Kitagawa, H., Yanagisawa, J., Yano, T., Kato, S. (2005). Repressive domain of unliganded human estrogen receptor {alpha} associates with Hsc70. GENES CELLS 10: 1095-1102 [Abstract] [Full Text]  
  • Lin, C., Yang, L., Yang, J. J., Huang, Y., Liu, Z.-R. (2005). ATPase/Helicase Activities of p68 RNA Helicase Are Required for Pre-mRNA Splicing but Not for Assembly of the Spliceosome. Mol. Cell. Biol. 25: 7484-7493 [Abstract] [Full Text]  
  • Takahashi, T., Ohmichi, M., Kawagoe, J., Ohshima, C., Doshida, M., Ohta, T., Saitoh, M., Mori-Abe, A., Du, B., Igarashi, H., Takahashi, K., Kurachi, H. (2005). Growth Factors Change Nuclear Distribution of Estrogen Receptor-{alpha} via Mitogen-Activated Protein Kinase or Phosphatidylinositol 3-Kinase Cascade in a Human Breast Cancer Cell Line. Endocrinology 146: 4082-4089 [Abstract] [Full Text]  
  • Auboeuf, D., Dowhan, D. H., Dutertre, M., Martin, N., Berget, S. M., O'Malley, B. W. (2005). A Subset of Nuclear Receptor Coregulators Act as Coupling Proteins during Synthesis and Maturation of RNA Transcripts. Mol. Cell. Biol. 25: 5307-5316 [Full Text]  
  • Matthews, J., Wihlen, B., Thomsen, J., Gustafsson, J.-A. (2005). Aryl Hydrocarbon Receptor-Mediated Transcription: Ligand-Dependent Recruitment of Estrogen Receptor {alpha} to 2,3,7,8-Tetrachlorodibenzo- p-Dioxin-Responsive Promoters. Mol. Cell. Biol. 25: 5317-5328 [Abstract] [Full Text]  
  • Masuhiro, Y., Mezaki, Y., Sakari, M., Takeyama, K.-i., Yoshida, T., Inoue, K., Yanagisawa, J., Hanazawa, S., O'Malley, B. W., Kato, S. (2005). Splicing potentiation by growth factor signals via estrogen receptor phosphorylation. Proc. Natl. Acad. Sci. USA 102: 8126-8131 [Abstract] [Full Text]  
  • Yang, L., Lin, C., Liu, Z.-R. (2005). Phosphorylations of DEAD Box p68 RNA Helicase Are Associated with Cancer Development and Cell Proliferation. Mol Cancer Res 3: 355-363 [Abstract] [Full Text]  
  • Gburcik, V., Bot, N., Maggiolini, M., Picard, D. (2005). SPBP Is a Phosphoserine-Specific Repressor of Estrogen Receptor {alpha}. Mol. Cell. Biol. 25: 3421-3430 [Abstract] [Full Text]  
  • Bour, G., Plassat, J.-L., Bauer, A., Lalevee, S., Rochette-Egly, C. (2005). Vinexin {beta} Interacts with the Non-phosphorylated AF-1 Domain of Retinoid Receptor {gamma} (RAR{gamma}) and Represses RAR{gamma}-mediated Transcription. J. Biol. Chem. 280: 17027-17037 [Abstract] [Full Text]  
  • Shah, Y. M., Rowan, B. G. (2005). The Src Kinase Pathway Promotes Tamoxifen Agonist Action in Ishikawa Endometrial Cells through Phosphorylation-Dependent Stabilization of Estrogen Receptor {alpha} Promoter Interaction and Elevated Steroid Receptor Coactivator 1 Activity. Mol. Endocrinol. 19: 732-748 [Abstract] [Full Text]  
  • Herynk, M. H., Fuqua, S. A. W. (2004). Estrogen Receptor Mutations in Human Disease. Endocr. Rev. 25: 869-898 [Abstract] [Full Text]  
  • Ito, S., Takeyama, K.-i., Yamamoto, A., Sawatsubashi, S., Shirode, Y., Kouzmenko, A., Tabata, T., Kato, S. (2004). In vivo potentiation of human oestrogen receptor {alpha} by Cdk7-mediated phosphorylation. GENES CELLS 9: 983-992 [Abstract] [Full Text]  
  • Yadav, V. K., Muraly, P., Medhamurthy, R. (2004). Identification of novel genes regulated by LH in the primate corpus luteum: insight into their regulation during the late luteal phase. Mol Hum Reprod 10: 629-639 [Abstract] [Full Text]  
  • Merot, Y., Metivier, R., Penot, G., Manu, D., Saligaut, C., Gannon, F., Pakdel, F., Kah, O., Flouriot, G. (2004). The Relative Contribution Exerted by AF-1 and AF-2 Transactivation Functions in Estrogen Receptor {alpha} Transcriptional Activity Depends upon the Differentiation Stage of the Cell. J. Biol. Chem. 279: 26184-26191 [Abstract] [Full Text]  
  • Goh, P.-Y., Tan, Y.-J., Lim, S. P., Tan, Y. H., Lim, S. G., Fuller-Pace, F., Hong, W. (2004). Cellular RNA Helicase p68 Relocalization and Interaction with the Hepatitis C Virus (HCV) NS5B Protein and the Potential Role of p68 in HCV RNA Replication. J. Virol. 78: 5288-5298 [Abstract] [Full Text]  
  • Smith, C. L., O'Malley, B. W. (2004). Coregulator Function: A Key to Understanding Tissue Specificity of Selective Receptor Modulators. Endocr. Rev. 25: 45-71 [Abstract] [Full Text]  
  • FOWLER, A. M., SOLODIN, N., PREISLER-MASHEK, M. T., ZHANG, P., LEE, A. V., ALARID, E. T. (2004). Increases in estrogen receptor-{alpha} concentration in breast cancer cells promote serine 118/104/106-independent AF-1 transactivation and growth in the absence of estrogen. FASEB J. 18: 81-93 [Abstract] [Full Text]  
  • Vallee, M., Beaudry, D., Roberge, C., Matte, J. J., Blouin, R., Palin, M.-F. (2003). Isolation of Differentially Expressed Genes in Conceptuses and Endometrial Tissue of Sows in Early Gestation. Biol. Reprod. 69: 1697-1706 [Abstract] [Full Text]  
  • Ding, L., Yan, J., Zhu, J., Zhong, H., Lu, Q., Wang, Z., Huang, C., Ye, Q. (2003). Ligand-independent activation of estrogen receptor {alpha} by XBP-1. Nucleic Acids Res 31: 5266-5274 [Abstract] [Full Text]  
  • Matthews, J., Gustafsson, J.-A. (2003). Estrogen Signaling: A Subtle Balance Between ER{alpha} and ER{beta}. Mol. Interv. 3: 281-292 [Abstract] [Full Text]  
  • Fujita, T., Kobayashi, Y., Wada, O., Tateishi, Y., Kitada, L., Yamamoto, Y., Takashima, H., Murayama, A., Yano, T., Baba, T., Kato, S., Kawabe, Y.-i., Yanagisawa, J. (2003). Full Activation of Estrogen Receptor {alpha} Activation Function-1 Induces Proliferation of Breast Cancer Cells. J. Biol. Chem. 278: 26704-26714 [Abstract] [Full Text]  
  • Dutertre, M., Smith, C. L. (2003). Ligand-Independent Interactions of p160/Steroid Receptor Coactivators and CREB-Binding Protein (CBP) with Estrogen Receptor-{alpha}: Regulation by Phosphorylation Sites in the A/B Region Depends on Other Receptor Domains. Mol. Endocrinol. 17: 1296-1314 [Abstract] [Full Text]  
  • Kim, K., Thu, N., Saville, B., Safe, S. (2003). Domains of Estrogen Receptor {alpha} (ER{alpha}) Required for ER{alpha}/Sp1-Mediated Activation of GC-Rich Promoters by Estrogens and Antiestrogens in Breast Cancer Cells. Mol. Endocrinol. 17: 804-817 [Abstract] [Full Text]  
  • Coleman, K. M., Dutertre, M., El-Gharbawy, A., Rowan, B. G., Weigel, N. L., Smith, C. L. (2003). Mechanistic Differences in the Activation of Estrogen Receptor-alpha (ERalpha )- and ERbeta -dependent Gene Expression by cAMP Signaling Pathway(s). J. Biol. Chem. 278: 12834-12845 [Abstract] [Full Text]  
  • Ogilvie, V. C., Wilson, B. J., Nicol, S. M., Morrice, N. A., Saunders, L. R., Barber, G. N., Fuller-Pace, F. V. (2003). The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells. Nucleic Acids Res 31: 1470-1480 [Abstract] [Full Text]  
  • Rajendran, R. R., Nye, A. C., Frasor, J., Balsara, R. D., Martini, P. G. V., Katzenellenbogen, B. S. (2003). Regulation of Nuclear Receptor Transcriptional Activity by a Novel DEAD Box RNA Helicase (DP97). J. Biol. Chem. 278: 4628-4638 [Abstract] [Full Text]  
  • Yan, X., Mouillet, J.-F., Ou, Q., Sadovsky, Y. (2003). A Novel Domain within the DEAD-Box Protein DP103 Is Essential for Transcriptional Repression and Helicase Activity. Mol. Cell. Biol. 23: 414-423 [Abstract] [Full Text]  
  • Ho, K. J., Liao, J. K. (2002). Nonnuclear Actions of Estrogen. Arterioscler. Thromb. Vasc. Bio. 22: 1952-1961 [Abstract] [Full Text]  
  • Bommer, M., Benecke, A., Gronemeyer, H., Rochette-Egly, C. (2002). TIF2 Mediates the Synergy between RARalpha 1 Activation Functions AF-1 and AF-2. J. Biol. Chem. 277: 37961-37966 [Abstract] [Full Text]  
  • Honig, A., Auboeuf, D., Parker, M. M., O'Malley, B. W., Berget, S. M. (2002). Regulation of Alternative Splicing by the ATP-Dependent DEAD-Box RNA Helicase p72. Mol. Cell. Biol. 22: 5698-5707 [Abstract] [Full Text]  
  • Lee, H., Bai, W. (2002). Regulation of Estrogen Receptor Nuclear Export by Ligand-Induced and p38-Mediated Receptor Phosphorylation. Mol. Cell. Biol. 22: 5835-5845 [Abstract] [Full Text]  
  • Liu, Z.-R. (2002). p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5' Splice Site Duplex. Mol. Cell. Biol. 22: 5443-5450 [Abstract] [Full Text]  
  • Jung, D.-J., Sung, H.-S., Goo, Y.-W., Lee, H. M., Park, O. K., Jung, S.-Y., Lim, J., Kim, H.-J., Lee, S.-K., Kim, T. S., Lee, J. W., Lee, Y. C. (2002). Novel Transcription Coactivator Complex Containing Activating Signal Cointegrator 1. Mol. Cell. Biol. 22: 5203-5211 [Abstract] [Full Text]  
  • Uhlmann-Schiffler, H., Seinsoth, S., Stahl, H. (2002). Preformed hexamers of SV40 T antigen are active in RNA and origin-DNA unwinding. Nucleic Acids Res 30: 3192-3201 [Abstract] [Full Text]  
  • Ho, K. J., Liao, J. K. (2002). Non-nuclear Actions of Estrogen: New Targets for Prevention and Treatment of Cardiovascular Disease. Mol. Interv. 2: 219-228 [Abstract] [Full Text]  
  • Kitagawa, H., Yanagisawa, J., Fuse, H., Ogawa, S., Yogiashi, Y., Okuno, A., Nagasawa, H., Nakajima, T., Matsumoto, T., Kato, S. (2002). Ligand-Selective Potentiation of Rat Mineralocorticoid Receptor Activation Function 1 by a CBP-Containing Histone Acetyltransferase Complex. Mol. Cell. Biol. 22: 3698-3706 [Abstract] [Full Text]  
  • Gay, F., Barath, P., Desbois-Le Peron, C., Metivier, R., Le Guevel, R., Birse, D., Salbert, G. (2002). Multiple Phosphorylation Events Control Chicken Ovalbumin Upstream Promoter Transcription Factor I Orphan Nuclear Receptor Activity. Mol. Endocrinol. 16: 1332-1351 [Abstract] [Full Text]  
  • Barkhem, T., Haldosen, L.-A., Gustafsson, J.-A., Nilsson, S. (2002). pS2 Gene Expression in HepG2 cells: Complex Regulation through Crosstalk between the Estrogen Receptor alpha , an Estrogen-Responsive Element, and the Activator Protein 1 Response Element. Mol. Pharmacol. 61: 1273-1283 [Abstract] [Full Text]  
  • Rouleau, N., Domans'kyi, A., Reeben, M., Moilanen, A.-M., Havas, K., Kang, Z., Owen-Hughes, T., Palvimo, J. J., Janne, O. A. (2002). Novel ATPase of SNF2-like Protein Family Interacts with Androgen Receptor and Modulates Androgen-dependent Transcription. Mol. Biol. Cell 13: 2106-2119 [Abstract] [Full Text]  
  • Huang, Y., Liu, Z.-R. (2002). The ATPase, RNA Unwinding, and RNA Binding Activities of Recombinant p68 RNA Helicase. J. Biol. Chem. 277: 12810-12815 [Abstract] [Full Text]  
  • Han, I., Xue, Y., Harada, S., Orstavik, S., Skalhegg, B., Kieff, E. (2002). Protein Kinase A Associates with HA95 and Affects Transcriptional Coactivation by Epstein-Barr Virus Nuclear Proteins. Mol. Cell. Biol. 22: 2136-2146 [Abstract] [Full Text]  
  • Hall, J. M., McDonnell, D. P., Korach, K. S. (2002). Allosteric Regulation of Estrogen Receptor Structure, Function, and Coactivator Recruitment by Different Estrogen Response Elements. Mol. Endocrinol. 16: 469-486 [Abstract] [Full Text]  
  • Klinge, C. M., Jernigan, S. C., Risinger, K. E. (2002). The Agonist Activity of Tamoxifen Is Inhibited by the Short Heterodimer Partner Orphan Nuclear Receptor in Human Endometrial Cancer Cells. Endocrinology 143: 853-867 [Abstract] [Full Text]  
  • Ezashi, T., Ghosh, D., Roberts, R. M. (2001). Repression of Ets-2-Induced Transactivation of the Tau Interferon Promoter by Oct-4. Mol. Cell. Biol. 21: 7883-7891 [Abstract] [Full Text]  
  • Yamamoto, Y., Wada, O., Suzawa, M., Yogiashi, Y., Yano, T., Kato, S., Yanagisawa, J. (2001). The Tamoxifen-responsive Estrogen Receptor alpha Mutant D351Y Shows Reduced Tamoxifen-dependent Interaction with Corepressor Complexes. J. Biol. Chem. 276: 42684-42691 [Abstract] [Full Text]  
  • Metivier, R., Penot, G., Flouriot, G., Pakdel, F. (2001). Synergism Between ER{alpha} Transactivation Function 1 (AF-1) and AF-2 Mediated by Steroid Receptor Coactivator Protein-1: Requirement for the AF-1 {alpha}-Helical Core and for a Direct Interaction Between the N- and C-Terminal Domains. Mol. Endocrinol. 15: 1953-1970 [Abstract] [Full Text]  
  • Nilsson, S., Makela, S., Treuter, E., Tujague, M., Thomsen, J., Andersson, G., Enmark, E., Pettersson, K., Warner, M., Gustafsson, J.-A. (2001). Mechanisms of Estrogen Action. Physiol. Rev. 81: 1535-1565 [Abstract] [Full Text]  
  • Yahata, T., Shao, W., Endoh, H., Hur, J., Coser, K. R., Sun, H., Ueda, Y., Kato, S., Isselbacher, K. J., Brown, M., Shioda, T. (2001). Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein. Genes Dev. 15: 2598-2612 [Abstract] [Full Text]  
  • Ishizuka, T., Satoh, T., Monden, T., Shibusawa, N., Hashida, T., Yamada, M., Mori, M. (2001). Human Immunodeficiency Virus Type 1 Tat Binding Protein-1 Is a Transcriptional Coactivator Specific for TR. Mol. Endocrinol. 15: 1329-1343 [Abstract] [Full Text]  
  • Conneely, O. M. (2001). Perspective: Female Steroid Hormone Action. Endocrinology 142: 2194-2199 [Full Text]  
  • Zhu, B., Benjamin, D., Zheng, Y., Angliker, H., Thiry, S., Siegmann, M., Jost, J.-P. (2001). Overexpression of 5-methylcytosine DNA glycosylase in human embryonic kidney cells EcR293 demethylates the promoter of a hormone-regulated reporter gene. Proc. Natl. Acad. Sci. USA 10.1073/pnas.091097298v1 [Abstract] [Full Text]  
  • Feng, W., Webb, P., Nguyen, P., Liu, X., Li, J., Karin, M., Kushner, P. J. (2001). Potentiation of Estrogen Receptor Activation Function 1 (AF-1) by Src/JNK through a Serine 118-Independent Pathway. Mol. Endocrinol. 15: 32-45 [Abstract] [Full Text]  
  • Ou, Q., Mouillet, J.-F., Yan, X., Dorn, C., Crawford, P. A., Sadovsky, Y. (2001). The DEAD Box Protein DP103 Is a Regulator of Steroidogenic Factor-1. Mol. Endocrinol. 15: 69-79 [Abstract] [Full Text]  
  • Falkenstein, E., Tillmann, H.-C., Christ, M., Feuring, M., Wehling, M. (2000). Multiple Actions of Steroid Hormones---A Focus on Rapid, Nongenomic Effects. Pharmacol. Rev. 52: 513-556 [Abstract] [Full Text]  
  • Dutertre, M., Smith, C. L. (2000). Molecular Mechanisms of Selective Estrogen Receptor Modulator (SERM) Action. J. Pharmacol. Exp. Ther. 295: 431-437 [Abstract] [Full Text]  
  • Métivier, R., Petit, F. G., Valotaire, Y., Pakdel, F. (2000). Function of N-Terminal Transactivation Domain of the Estrogen Receptor Requires a Potential {alpha}-Helical Structure and Is Negatively Regulated by the A Domain. Mol. Endocrinol. 14: 1849-1871 [Abstract] [Full Text]  
  • Watters, J. J., Chun, T.-Y., Kim, Y.-N., Bertics, P. J., Gorski, J. (2000). Estrogen Modulation of Prolactin Gene Expression Requires an Intact Mitogen-Activated Protein Kinase Signal Transduction Pathway in Cultured Rat Pituitary Cells. Mol. Endocrinol. 14: 1872-1881 [Abstract] [Full Text]  
  • Fuse, H., Kitagawa, H., Kato, S. (2000). Characterization of Transactivational Property and Coactivator Mediation of Rat Mineralocorticoid Receptor Activation Function-1 (AF-1). Mol. Endocrinol. 14: 889-899 [Abstract] [Full Text]  
  • Saville, B., Wormke, M., Wang, F., Nguyen, T., Enmark, E., Kuiper, G., Gustafsson, J.-A., Safe, S. (2000). Ligand-, Cell-, and Estrogen Receptor Subtype (alpha /beta )-dependent Activation at GC-rich (Sp1) Promoter Elements. J. Biol. Chem. 275: 5379-5387 [Abstract] [Full Text]  
  • Rossler, O. G., Hloch, P., Schutz, N., Weitzenegger, T., Stahl, H. (2000). Structure and expression of the human p68 RNA helicase gene. Nucleic Acids Res 28: 932-939 [Abstract] [Full Text]  
  • Kurebayashi, J., Otsuki, T., Kunisue, H., Tanaka, K., Yamamoto, S., Sonoo, H. (2000). Expression Levels of Estrogen Receptor-{{alpha}}, Estrogen Receptor-{beta}, Coactivators, and Corepressors in Breast Cancer. Clin. Cancer Res. 6: 512-518 [Abstract] [Full Text]  
  • Su, L. F., Knoblauch, R., Garabedian, M. J. (2001). Rho GTPases as Modulators of the Estrogen Receptor Transcriptional Response. J. Biol. Chem. 276: 3231-3237 [Abstract] [Full Text]  
  • Knotts, T. A., Orkiszewski, R. S., Cook, R. G., Edwards, D. P., Weigel, N. L. (2001). Identification of a Phosphorylation Site in the Hinge Region of the Human Progesterone Receptor and Additional Amino-terminal Phosphorylation Sites. J. Biol. Chem. 276: 8475-8483 [Abstract] [Full Text]  
  • Lopez, G. N., Turck, C. W., Schaufele, F., Stallcup, M. R., Kushner, P. J. (2001). Growth Factors Signal to Steroid Receptors through Mitogen-activated Protein Kinase Regulation of p160 Coactivator Activity. J. Biol. Chem. 276: 22177-22182 [Abstract] [Full Text]  
  • Campbell, R. A., Bhat-Nakshatri, P., Patel, N. M., Constantinidou, D., Ali, S., Nakshatri, H. (2001). Phosphatidylinositol 3-Kinase/AKT-mediated Activation of Estrogen Receptor alpha . A NEW MODEL FOR ANTI-ESTROGEN RESISTANCE. J. Biol. Chem. 276: 9817-9824 [Abstract] [Full Text]  
  • Wang, C., Fu, M., Angeletti, R. H., Siconolfi-Baez, L., Reutens, A. T., Albanese, C., Lisanti, M. P., Katzenellenbogen, B. S., Kato, S., Hopp, T., Fuqua, S. A. W., Lopez, G. N., Kushner, P. J., Pestell, R. G. (2001). Direct Acetylation of the Estrogen Receptor alpha Hinge Region by p300 Regulates Transactivation and Hormone Sensitivity. J. Biol. Chem. 276: 18375-18383 [Abstract] [Full Text]  
  • Wu, X., Li, H., Chen, J. D. (2001). The Human Homologue of the Yeast DNA Repair and TFIIH Regulator MMS19 Is an AF-1-specific Coactivator of Estrogen Receptor. J. Biol. Chem. 276: 23962-23968 [Abstract] [Full Text]  
  • Akileswaran, L., Taraska, J. W., Sayer, J. A., Gettemy, J. M., Coghlan, V. M. (2001). A-kinase-anchoring Protein AKAP95 Is Targeted to the Nuclear Matrix and Associates with p68 RNA Helicase. J. Biol. Chem. 276: 17448-17454 [Abstract] [Full Text]  
  • Song, L.-N., Huse, B., Rusconi, S., Simons, S. S. Jr. (2001). Transactivation Specificity of Glucocorticoid Versus Progesterone Receptors. ROLE OF FUNCTIONALLY DIFFERENT INTERACTIONS OF TRANSCRIPTION FACTORS WITH AMINO- AND CARBOXYL-TERMINAL RECEPTOR DOMAINS. J. Biol. Chem. 276: 24806-24816 [Abstract] [Full Text]  
  • Uhlmann-Schiffler, H., Rossler, O. G., Stahl, H. (2002). The mRNA of DEAD Box Protein p72 Is Alternatively Translated into an 82-kDa RNA Helicase. J. Biol. Chem. 277: 1066-1075 [Abstract] [Full Text]  
  • Saville, B., Poukka, H., Wormke, M., Janne, O. A., Palvimo, J. J., Stoner, M., Samudio, I., Safe, S. (2002). Cooperative Coactivation of Estrogen Receptor alpha in ZR-75 Human Breast Cancer Cells by SNURF and TATA-binding Protein. J. Biol. Chem. 277: 2485-2497 [Abstract] [Full Text]  
  • Jung, D.-J., Na, S.-Y., Na, D. S., Lee, J. W. (2002). Molecular Cloning and Characterization of CAPER, a Novel Coactivator of Activating Protein-1 and Estrogen Receptors. J. Biol. Chem. 277: 1229-1234 [Abstract] [Full Text]  
  • Zhu, B., Benjamin, D., Zheng, Y., Angliker, H., Thiry, S., Siegmann, M., Jost, J.-P. (2001). Overexpression of 5-methylcytosine DNA glycosylase in human embryonic kidney cells EcR293 demethylates the promoter of a hormone-regulated reporter gene. Proc. Natl. Acad. Sci. USA 98: 5031-5036 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»