This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rep, M.
Right arrow Articles by Ruis, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rep, M.
Right arrow Articles by Ruis, H.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 1999, p. 5474-5485, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Osmotic Stress-Induced Gene Expression in Saccharomyces cerevisiae Requires Msn1p and the Novel Nuclear Factor Hot1p

Martijn Rep,1 Vladimír Reiser,2 Ulrike Gartner,2 Johan M. Thevelein,1 Stefan Hohmann,1,3,* Gustav Ammerer,2 and Helmut Ruis2

Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, B-3001 Heverlee, Flanders, Belgium1; Institute of Biochemistry and Molecular Cell Biology and Ludwig Boltzmann-Forschungsstelle für Biochemie, University of Vienna, A-1030 Vienna, Austria2; and Department of Cell and Molecular Biology/Microbiology, Lundberg Laboratory, Göteborg University, S-405 30 Göteborg, Sweden3

Received 3 March 1999/Returned for modification 8 April 1999/Accepted 28 April 1999

After a sudden shift to high osmolarity, Saccharomyces cerevisiae cells respond by transiently inducing the expression of stress-protective genes. Msn2p and Msn4p have been described as two transcription factors that determine the extent of this response. In msn2 msn4 mutants, however, many promoters still show a distinct rise in transcriptional activity upon osmotic stress. Here we describe two structurally related nuclear factors, Msn1p and a newly identified protein, Hot1p (for high-osmolarity-induced transcription), which are also involved in osmotic stress-induced transcription. hot1 single mutants are specifically compromised in the transient induction of GPD1 and GPP2, which encode enzymes involved in glycerol biosynthesis, and exhibit delayed glycerol accumulation after stress exposure. Similar to a gpd1 mutation, a hot1 defect can rescue cells from inappropriately high HOG pathway activity. In contrast, Hot1p has little influence on the osmotic stress induction of CTT1, where Msn1p appears to play a more prominent role. Cells lacking Msn1p, Msn2p, Msn4p, and Hot1p are almost devoid of the short-term transcriptional response of the genes GPD1, GPP2, CTT1, and HSP12 to osmotic stress. Such cells also show a distinct reduction in the nuclear residence of the mitogen-activated protein kinase Hog1p upon osmotic stress. Thus, Hot1p and Msn1p may define an additional tier of transcriptional regulators that control responses to high-osmolarity stress.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology/Microbiology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden. Phone: 46-31-7732595. Fax: 46-31-7732599. E-mail: hohmann{at}gmm.gu.se.

Molecular and Cellular Biology, August 1999, p. 5474-5485, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Motizuki, M., Xu, Z. (2009). Importance of Polarisome Proteins in Reorganization of Actin Cytoskeleton at Low pH in Saccharomyces cerevisiae. J Biochem 146: 705-712 [Abstract] [Full Text]  
  • Ni, L., Bruce, C., Hart, C., Leigh-Bell, J., Gelperin, D., Umansky, L., Gerstein, M. B., Snyder, M. (2009). Dynamic and complex transcription factor binding during an inducible response in yeast. Genes Dev. 23: 1351-1363 [Abstract] [Full Text]  
  • Rosonina, E., Willis, I. M., Manley, J. L. (2009). Sub1 Functions in Osmoregulation and in Transcription by both RNA Polymerases II and III. Mol. Cell. Biol. 29: 2308-2321 [Abstract] [Full Text]  
  • Molin, C., Jauhiainen, A., Warringer, J., Nerman, O., Sunnerhagen, P. (2009). mRNA stability changes precede changes in steady-state mRNA amounts during hyperosmotic stress. RNA 15: 600-614 [Abstract] [Full Text]  
  • Bahn, Y.-S. (2008). Master and Commander in Fungal Pathogens: the Two-Component System and the HOG Signaling Pathway. Eukaryot Cell 7: 2017-2036 [Full Text]  
  • Westfall, P. J., Patterson, J. C., Chen, R. E., Thorner, J. (2008). Stress resistance and signal fidelity independent of nuclear MAPK function. Proc. Natl. Acad. Sci. USA 105: 12212-12217 [Abstract] [Full Text]  
  • Melamed, D., Pnueli, L., Arava, Y. (2008). Yeast translational response to high salinity: Global analysis reveals regulation at multiple levels. RNA 14: 1337-1351 [Abstract] [Full Text]  
  • Bermejo, C., Rodriguez, E., Garcia, R., Rodriguez-Pena, J. M., Rodriguez de la Concepcion, M. L., Rivas, C., Arias, P., Nombela, C., Posas, F., Arroyo, J. (2008). The Sequential Activation of the Yeast HOG and SLT2 Pathways Is Required for Cell Survival to Cell Wall Stress. Mol. Biol. Cell 19: 1113-1124 [Abstract] [Full Text]  
  • Ye, T., Garcia-Salcedo, R., Ramos, J., Hohmann, S. (2006). Gis4, a New Component of the Ion Homeostasis System in the Yeast Saccharomyces cerevisiae.. Eukaryot Cell 5: 1611-1621 [Abstract] [Full Text]  
  • Page, M. J., Di Cera, E. (2006). Role of na+ and k+ in enzyme function.. Physiol. Rev. 86: 1049-1092 [Abstract] [Full Text]  
  • Thorsen, M., Di, Y., Tangemo, C., Morillas, M., Ahmadpour, D., Van der Does, C., Wagner, A., Johansson, E., Boman, J., Posas, F., Wysocki, R., Tamas, M. J. (2006). The MAPK Hog1p Modulates Fps1p-dependent Arsenite Uptake and Tolerance in Yeast. Mol. Biol. Cell 17: 4400-4410 [Abstract] [Full Text]  
  • Marques, J. M., Rodrigues, R. J., de Magalhaes-Sant'Ana, A. C., Goncalves, T. (2006). Saccharomyces cerevisiae Hog1 Protein Phosphorylation upon Exposure to Bacterial Endotoxin. J. Biol. Chem. 281: 24687-24694 [Abstract] [Full Text]  
  • Reiser, V., D'Aquino, K. E., Ee, L.-S., Amon, A. (2006). The Stress-activated Mitogen-activated Protein Kinase Signaling Cascade Promotes Exit from Mitosis. Mol. Biol. Cell 17: 3136-3146 [Abstract] [Full Text]  
  • Jiang, R., Tu, Z., Chen, T., Sun, F. (2006). Network motif identification in stochastic networks. Proc. Natl. Acad. Sci. USA 103: 9404-9409 [Abstract] [Full Text]  
  • Krasley, E., Cooper, K. F., Mallory, M. J., Dunbrack, R., Strich, R. (2006). Regulation of the Oxidative Stress Response Through Slt2p-Dependent Destruction of Cyclin C in Saccharomyces cerevisiae. Genetics 172: 1477-1486 [Abstract] [Full Text]  
  • Santos, A., del Mar Alvarez, M., Mauro, M. S., Abrusci, C., Marquina, D. (2005). The Transcriptional Response of Saccharomyces cerevisiae to Pichia membranifaciens Killer Toxin. J. Biol. Chem. 280: 41881-41892 [Abstract] [Full Text]  
  • Vyas, V. K., Berkey, C. D., Miyao, T., Carlson, M. (2005). Repressors Nrg1 and Nrg2 Regulate a Set of Stress-Responsive Genes in Saccharomyces cerevisiae. Eukaryot Cell 4: 1882-1891 [Abstract] [Full Text]  
  • Proft, M., Gibbons, F. D., Copeland, M., Roth, F. P., Struhl, K. (2005). Genomewide Identification of Sko1 Target Promoters Reveals a Regulatory Network That Operates in Response to Osmotic Stress in Saccharomyces cerevisiae. Eukaryot Cell 4: 1343-1352 [Abstract] [Full Text]  
  • Edmunds, J. W., Mahadevan, L. C. (2004). MAP kinases as structural adaptors and enzymatic activators in transcription complexes. J. Cell Sci. 117: 3715-3723 [Abstract] [Full Text]  
  • Saito, H., Tatebayashi, K. (2004). Regulation of the Osmoregulatory HOG MAPK Cascade in Yeast. J Biochem 136: 267-272 [Abstract] [Full Text]  
  • Wolfger, H., Mamnun, Y. M., Kuchler, K. (2004). The Yeast Pdr15p ATP-binding Cassette (ABC) Protein Is a General Stress Response Factor Implicated in Cellular Detoxification. J. Biol. Chem. 279: 11593-11599 [Abstract] [Full Text]  
  • O'Rourke, S. M., Herskowitz, I. (2004). Unique and Redundant Roles for HOG MAPK Pathway Components as Revealed by Whole-Genome Expression Analysis. Mol. Biol. Cell 15: 532-542 [Abstract] [Full Text]  
  • Goodwin, T. J. D., Butler, M. I., Poulter, R. T. M. (2003). Cryptons: a group of tyrosine-recombinase-encoding DNA transposons from pathogenic fungi. Microbiology 149: 3099-3109 [Abstract] [Full Text]  
  • Sato, N., Kawahara, H., Toh-e, A., Maeda, T. (2003). Phosphorelay-Regulated Degradation of the Yeast Ssk1p Response Regulator by the Ubiquitin-Proteasome System. Mol. Cell. Biol. 23: 6662-6671 [Abstract] [Full Text]  
  • Rodriguez-Hernandez, C. J., Sanchez-Perez, I., Gil-Mascarell, R., Rodriguez-Afonso, A., Torres, A., Perona, R., Murguia, J. R. (2003). The Immunosuppressant FK506 Uncovers a Positive Regulatory Cross-talk between the Hog1p and Gcn2p Pathways. J. Biol. Chem. 278: 33887-33895 [Abstract] [Full Text]  
  • Yaakov, G., Bell, M., Hohmann, S., Engelberg, D. (2003). Combination of Two Activating Mutations in One HOG1 Gene Forms Hyperactive Enzymes That Induce Growth Arrest. Mol. Cell. Biol. 23: 4826-4840 [Abstract] [Full Text]  
  • Wojda, I., Alonso-Monge, R., Bebelman, J.-P., Mager, W. H., Siderius, M. (2003). Response to high osmotic conditions and elevated temperature in Saccharomyces cerevisiae is controlled by intracellular glycerol and involves coordinate activity of MAP kinase pathways. Microbiology 149: 1193-1204 [Abstract] [Full Text]  
  • Enjalbert, B., Nantel, A., Whiteway, M. (2003). Stress-induced Gene Expression in Candida albicans: Absence of a General Stress Response. Mol. Biol. Cell 14: 1460-1467 [Abstract] [Full Text]  
  • Page, N., Gerard-Vincent, M., Menard, P., Beaulieu, M., Azuma, M., Dijkgraaf, G. J. P., Li, H., Marcoux, J., Nguyen, T., Dowse, T., Sdicu, A.-M., Bussey, H. (2003). A Saccharomyces cerevisiae Genome-Wide Mutant Screen for Altered Sensitivity to K1 Killer Toxin. Genetics 163: 875-894 [Abstract] [Full Text]  
  • Nadal, E. d., Casadome, L., Posas, F. (2003). Targeting the MEF2-Like Transcription Factor Smp1 by the Stress-Activated Hog1 Mitogen-Activated Protein Kinase. Mol. Cell. Biol. 23: 229-237 [Abstract] [Full Text]  
  • Kaeberlein, M., Andalis, A. A., Fink, G. R., Guarente, L. (2002). High Osmolarity Extends Life Span in Saccharomyces cerevisiae by a Mechanism Related to Calorie Restriction. Mol. Cell. Biol. 22: 8056-8066 [Abstract] [Full Text]  
  • Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  
  • Teige, M., Scheikl, E., Reiser, V., Ruis, H., Ammerer, G. (2001). Rck2, a member of the calmodulin-protein kinase family, links protein synthesis to high osmolarity MAP kinase signaling in budding yeast. Proc. Natl. Acad. Sci. USA 98: 5625-5630 [Abstract] [Full Text]  
  • Causton, H. C., Ren, B., Koh, S. S., Harbison, C. T., Kanin, E., Jennings, E. G., Lee, T. I., True, H. L., Lander, E. S., Young, R. A. (2001). Remodeling of Yeast Genome Expression in Response to Environmental Changes. Mol. Biol. Cell 12: 323-337 [Abstract] [Full Text]  
  • Pascual-Ahuir, A., Serrano, R., Proft, M. (2001). The Sko1p Repressor and Gcn4p Activator Antagonistically Modulate Stress-Regulated Transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 16-25 [Abstract] [Full Text]  
  • Gasch, A. P., Spellman, P. T., Kao, C. M., Carmel-Harel, O., Eisen, M. B., Storz, G., Botstein, D., Brown, P. O. (2000). Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes. Mol. Biol. Cell 11: 4241-4257 [Abstract] [Full Text]  
  • Garreau, H., Hasan, R. N., Renault, G., Estruch, F., Boy-Marcotte, E., Jacquet, M. (2000). Hyperphosphorylation of Msn2p and Msn4p in response to heat shock and the diauxic shift is inhibited by cAMP in Saccharomyces cerevisiae. Microbiology 146: 2113-2120 [Abstract] [Full Text]  
  • Garcia-Gimeno, M. A., Struhl, K. (2000). Aca1 and Aca2, ATF/CREB Activators in Saccharomyces cerevisiae, Are Important for Carbon Source Utilization but Not the Response to Stress. Mol. Cell. Biol. 20: 4340-4349 [Abstract] [Full Text]  
  • Rep, M., Krantz, M., Thevelein, J. M., Hohmann, S. (2000). The Transcriptional Response of Saccharomyces cerevisiae to Osmotic Shock. Hot1p AND Msn2p/Msn4p ARE REQUIRED FOR THE INDUCTION OF SUBSETS OF HIGH OSMOLARITY GLYCEROL PATHWAY-DEPENDENT GENES. J. Biol. Chem. 275: 8290-8300 [Abstract] [Full Text]  
  • Eriksson, P., Alipour, H., Adler, L., Blomberg, A. (2000). Rap1p-binding Sites in the Saccharomyces cerevisiae GPD1 Promoter Are Involved in Its Response to NaCl. J. Biol. Chem. 275: 29368-29376 [Abstract] [Full Text]  
  • Pahlman, A.-K., Granath, K., Ansell, R., Hohmann, S., Adler, L. (2001). The Yeast Glycerol 3-Phosphatases Gpp1p and Gpp2p Are Required for Glycerol Biosynthesis and Differentially Involved in the Cellular Responses to Osmotic, Anaerobic, and Oxidative Stress. J. Biol. Chem. 276: 3555-3563 [Abstract] [Full Text]  
  • Yale, J., Bohnert, H. J. (2001). Transcript Expression in Saccharomyces cerevisiae at High Salinity. J. Biol. Chem. 276: 15996-16007 [Abstract] [Full Text]  
  • Posas, F., Chambers, J. R., Heyman, J. A., Hoeffler, J. P., de Nadal, E., Arino, J. (2000). The Transcriptional Response of Yeast to Saline Stress. J. Biol. Chem. 275: 17249-17255 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»