This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balasundaram, D.
Right arrow Articles by Levin, H. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balasundaram, D.
Right arrow Articles by Levin, H. L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 1999, p. 5768-5784, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Nup124p Is a Nuclear Pore Factor of Schizosaccharomyces pombe That Is Important for Nuclear Import and Activity of Retrotransposon Tf1

David Balasundaram,1 Michael J. Benedik,2 Mary Morphew,3 Van-Dinh Dang,1 and Henry L. Levin1,*

Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland,1 Biochemical and Biophysical Sciences, University of Houston, Houston, Texas,2 and Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado3

Received 12 February 1999/Returned for modification 25 March 1999/Accepted 27 April 1999

The long terminal repeat (LTR)-containing retrotransposon Tf1 propagates within the fission yeast Schizosaccharomyces pombe as the result of several mechanisms that are typical of both retrotransposons and retroviruses. To identify host factors that contribute to the transposition process, we mutagenized cultures of S. pombe and screened them for strains that were unable to support Tf1 transposition. One such strain contained a mutation in a gene we named nup124. The product of this gene contains 11 FXFG repeats and is a component of the nuclear pore complex. In addition to the reduced levels of Tf1 transposition, the nup124-1 allele caused a significant reduction in the nuclear localization of Tf1 Gag. Surprisingly, the mutation in nup124-1 did not cause any reduction in the growth rate, the nuclear localization of specific nuclear localization signal-containing proteins, or the cytoplasmic localization of poly(A) mRNA. A two-hybrid analysis and an in vitro precipitation assay both identified an interaction between Tf1 Gag and the N terminus of Nup124p. These results provide evidence for an unusual mechanism of nuclear import that relies on a direct interaction between a nuclear pore factor and Tf1 Gag.

* Corresponding author. Mailing address: Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892. Phone: (301) 402-4281. Fax: (301) 496-8576. E-mail: Henry_Levin{at}nih.gov.

Molecular and Cellular Biology, August 1999, p. 5768-5784, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Beliakova-Bethell, N., Terry, L. J., Bilanchone, V., DaSilva, R., Nagashima, K., Wente, S. R., Sandmeyer, S. (2009). Ty3 Nuclear Entry Is Initiated by Viruslike Particle Docking on GLFG Nucleoporins. J. Virol. 83: 11914-11925 [Abstract] [Full Text]  
  • Low, C. P., Shui, G., Liew, L. P., Buttner, S., Madeo, F., Dawes, I. W., Wenk, M. R., Yang, H. (2008). Caspase-dependent and -independent lipotoxic cell-death pathways in fission yeast. J. Cell Sci. 121: 2671-2684 [Abstract] [Full Text]  
  • Sistla, S., Pang, J. V., Wang, C. X., Balasundaram, D. (2007). Multiple Conserved Domains of the Nucleoporin Nup124p and Its Orthologs Nup1p and Nup153 Are Critical for Nuclear Import and Activity of the Fission Yeast Tf1 Retrotransposon. Mol. Biol. Cell 18: 3692-3708 [Abstract] [Full Text]  
  • Levesque, L., Bor, Y.-C., Matzat, L. H., Jin, L., Berberoglu, S., Rekosh, D., Hammarskjold, M.-L., Paschal, B. M. (2006). Mutations in Tap Uncouple RNA Export Activity from Translocation through the Nuclear Pore Complex. Mol. Biol. Cell 17: 931-943 [Abstract] [Full Text]  
  • Du, X., Rao, M. R.K. S., Chen, X. Q., Wu, W., Mahalingam, S., Balasundaram, D. (2006). The Homologous Putative GTPases Grn1p from Fission Yeast and the Human GNL3L Are Required for Growth and Play a Role in Processing of Nucleolar Pre-rRNA. Mol. Biol. Cell 17: 460-474 [Abstract] [Full Text]  
  • Kim, M.-K., Claiborn, K. C., Levin, H. L. (2005). The Long Terminal Repeat-Containing Retrotransposon Tf1 Possesses Amino Acids in Gag That Regulate Nuclear Localization and Particle Formation. J. Virol. 79: 9540-9555 [Abstract] [Full Text]  
  • Varadarajan, P., Mahalingam, S., Liu, P., Ng, S. B. H., Gandotra, S., Dorairajoo, D. S. K., Balasundaram, D. (2005). The Functionally Conserved Nucleoporins Nup124p from Fission Yeast and the Human Nup153 Mediate Nuclear Import and Activity of the Tf1 Retrotransposon and HIV-1 Vpr. Mol. Biol. Cell 16: 1823-1838 [Abstract] [Full Text]  
  • Aye, M., Irwin, B., Beliakova-Bethell, N., Chen, E., Garrus, J., Sandmeyer, S. (2004). Host Factors That Affect Ty3 Retrotransposition in Saccharomyces cerevisiae. Genetics 168: 1159-1176 [Abstract] [Full Text]  
  • Wang, H., Tang, X., Balasubramanian, M. K. (2003). Rho3p Regulates Cell Separation by Modulating Exocyst Function in Schizosaccharomyces pombe. Genetics 164: 1323-1331 [Abstract] [Full Text]  
  • Teysset, L., Dang, V.-D., Kim, M. K., Levin, H. L. (2003). A Long Terminal Repeat-Containing Retrotransposon of Schizosaccharomyces pombe Expresses a Gag-Like Protein That Assembles into Virus-Like Particles Which Mediate Reverse Transcription. J. Virol. 77: 5451-5463 [Abstract] [Full Text]  
  • Singleton, T. L., Levin, H. L. (2002). A Long Terminal Repeat Retrotransposon of Fission Yeast Has Strong Preferences for Specific Sites of Insertion. Eukaryot Cell 1: 44-55 [Abstract] [Full Text]  
  • Dang, V.-D., Levin, H. L. (2000). Nuclear Import of the Retrotransposon Tf1 Is Governed by a Nuclear Localization Signal That Possesses a Unique Requirement for the FXFG Nuclear Pore Factor Nup124p. Mol. Cell. Biol. 20: 7798-7812 [Abstract] [Full Text]  
  • Wang, H., Tang, X., Liu, J., Trautmann, S., Balasundaram, D., McCollum, D., Balasubramanian, M. K. (2002). The Multiprotein Exocyst Complex Is Essential for Cell Separation in Schizosaccharomyces pombe. Mol. Biol. Cell 13: 515-529 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»