This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wallberg, A. E.
Right arrow Articles by Grant, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wallberg, A. E.
Right arrow Articles by Grant, P. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 1999, p. 5952-5959, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Histone Acetyltransferase Complexes Can Mediate Transcriptional Activation by the Major Glucocorticoid Receptor Activation Domain

Annika E. Wallberg,1,* Kristen E. Neely,2 Jan-Åke Gustafsson,1 Jerry L. Workman,2 Anthony P. H. Wright,1,3 and Patrick A. Grant2

Karolinska Institute, Department of Biosciences, NOVUM, S-14157 Huddinge,1 and Södertörns Högskola, S-14104 Huddinge,3 Sweden, and Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802-45002

Received 18 February 1999/Returned for modification 15 April 1999/Accepted 18 June 1999

Previous studies have shown that the Ada adapter proteins are important for glucocorticoid receptor (GR)-mediated gene activation in yeast. The N-terminal transactivation domain of GR, tau 1, is dependent upon Ada2, Ada3, and Gcn5 for transactivation in vitro and in vivo. Using in vitro techniques, we demonstrate that the GR-tau 1 interacts directly with the native Ada containing histone acetyltransferase (HAT) complex SAGA but not the related Ada complex. Mutations in tau 1 that reduce tau 1 transactivation activity in vivo lead to a reduced binding of tau 1 to the SAGA complex and conversely, mutations increasing the transactivation activity of tau 1 lead to an increased binding of tau 1 to SAGA. In addition, the Ada-independent NuA4 HAT complex also interacts with tau 1. GAL4-tau 1-driven transcription from chromatin templates is stimulated by SAGA and NuA4 in an acetyl coenzyme A-dependent manner. Low-activity tau 1 mutants reduce SAGA- and NuA4-stimulated transcription while high-activity tau 1 mutants increase transcriptional activation, specifically from chromatin templates. Our results demonstrate that the targeting of native HAT complexes by the GR-tau 1 activation domain mediates transcriptional stimulation from chromatin templates.

* Corresponding author. Mailing address: Karolinska Institute, Department of Biosciences, NOVUM, S-14157 Huddinge, Sweden. Phone: 46-8-6089155. Fax: 46-8-7745538. E-mail: annika.wallberg{at}csb.ki.se.

Molecular and Cellular Biology, September 1999, p. 5952-5959, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zsindely, N., Pankotai, T., Ujfaludi, Z., Lakatos, D., Komonyi, O., Bodai, L., Tora, L., Boros, I. M. (2009). The loss of histone H3 lysine 9 acetylation due to dSAGA-specific dAda2b mutation influences the expression of only a small subset of genes. Nucleic Acids Res 37: 6665-6680 [Abstract] [Full Text]  
  • Kim, D.-H., Kim, G. S., Yun, C. H., Lee, Y. C. (2008). Functional Conservation of the Glutamine-Rich Domains of Yeast Gal11 and Human SRC-1 in the Transactivation of Glucocorticoid Receptor Tau 1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 28: 913-925 [Abstract] [Full Text]  
  • Johnsson, A., Xue-Franzen, Y., Lundin, M., Wright, A. P. H. (2006). Stress-specific role of fission yeast gcn5 histone acetyltransferase in programming a subset of stress response genes.. Eukaryot Cell 5: 1337-1346 [Abstract] [Full Text]  
  • Martin, D. G. E., Grimes, D. E., Baetz, K., Howe, L. (2006). Methylation of Histone H3 Mediates the Association of the NuA3 Histone Acetyltransferase with Chromatin. Mol. Cell. Biol. 26: 3018-3028 [Abstract] [Full Text]  
  • Meng, G., Zhao, Y., Nag, A., Zeng, M., Dimri, G., Gao, Q., Wazer, D. E., Kumar, R., Band, H., Band, V. (2004). Human ADA3 Binds to Estrogen Receptor (ER) and Functions As a Coactivator for ER-mediated Transactivation. J. Biol. Chem. 279: 54230-54240 [Abstract] [Full Text]  
  • De Bosscher, K., Vanden Berghe, W., Haegeman, G. (2003). The Interplay between the Glucocorticoid Receptor and Nuclear Factor-{kappa}B or Activator Protein-1: Molecular Mechanisms for Gene Repression. Endocr. Rev. 24: 488-522 [Abstract] [Full Text]  
  • Hardy, S., Brand, M., Mittler, G., Yanagisawa, J., Kato, S., Meisterernst, M., Tora, L. (2002). TATA-binding Protein-free TAF-containing Complex (TFTC) and p300 Are Both Required for Efficient Transcriptional Activation. J. Biol. Chem. 277: 32875-32882 [Abstract] [Full Text]  
  • Keeton, E. K., Fletcher, T. M., Baumann, C. T., Hager, G. L., Smith, C. L. (2002). Glucocorticoid Receptor Domain Requirements for Chromatin Remodeling and Transcriptional Activation of the Mouse Mammary Tumor Virus Promoter in Different Nucleoprotein Contexts. J. Biol. Chem. 277: 28247-28255 [Abstract] [Full Text]  
  • Flinn, E. M., Wallberg, A. E., Hermann, S., Grant, P. A., Workman, J. L., Wright, A. P. H. (2002). Recruitment of Gcn5-containing Complexes during c-Myc-dependent Gene Activation. STRUCTURE AND FUNCTION ASPECTS. J. Biol. Chem. 277: 23399-23406 [Abstract] [Full Text]  
  • Benecke, A., Gaudon, C., Garnier, J.-M., vom Baur, E., Chambon, P., Losson, R. (2002). ADA3-containing complexes associate with estrogen receptor alpha. Nucleic Acids Res 30: 2508-2514 [Abstract] [Full Text]  
  • Zhu, Q., Dudley, J. P. (2002). CDP Binding to Multiple Sites in the Mouse Mammary Tumor Virus Long Terminal Repeat Suppresses Basal and Glucocorticoid-Induced Transcription. J. Virol. 76: 2168-2179 [Abstract] [Full Text]  
  • Martinez, E., Palhan, V. B., Tjernberg, A., Lymar, E. S., Gamper, A. M., Kundu, T. K., Chait, B. T., Roeder, R. G. (2001). Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In Vivo. Mol. Cell. Biol. 21: 6782-6795 [Abstract] [Full Text]  
  • Larschan, E., Winston, F. (2001). The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4. Genes Dev. 15: 1946-1956 [Abstract] [Full Text]  
  • Zhang, W., Kadam, S., Emerson, B. M., Bieker, J. J. (2001). Site-Specific Acetylation by p300 or CREB Binding Protein Regulates Erythroid Kruppel-Like Factor Transcriptional Activity via Its Interaction with the SWI-SNF Complex. Mol. Cell. Biol. 21: 2413-2422 [Abstract] [Full Text]  
  • Harbour, J. W., Dean, D. C. (2000). The Rb/E2F pathway: expanding roles and emerging paradigms. Genes Dev. 14: 2393-2409 [Full Text]  
  • Nasrin, N., Ogg, S., Cahill, C. M., Biggs, W., Nui, S., Dore, J., Calvo, D., Shi, Y., Ruvkun, G., Alexander-Bridges, M. C. (2000). DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in HepG2 cells. Proc. Natl. Acad. Sci. USA 10.1073/pnas.190326997v1 [Abstract] [Full Text]  
  • Sterner, D. E., Berger, S. L. (2000). Acetylation of Histones and Transcription-Related Factors. Microbiol. Mol. Biol. Rev. 64: 435-459 [Abstract] [Full Text]  
  • Wallberg, A. E., Neely, K. E., Hassan, A. H., Gustafsson, J.-A., Workman, J. L., Wright, A. P. H. (2000). Recruitment of the SWI-SNF Chromatin Remodeling Complex as a Mechanism of Gene Activation by the Glucocorticoid Receptor tau 1 Activation Domain. Mol. Cell. Biol. 20: 2004-2013 [Abstract] [Full Text]  
  • Eisen, A., Utley, R. T., Nourani, A., Allard, S., Schmidt, P., Lane, W. S., Lucchesi, J. C., Cote, J. (2001). The Yeast NuA4 and Drosophila MSL Complexes Contain Homologous Subunits Important for Transcription Regulation. J. Biol. Chem. 276: 3484-3491 [Abstract] [Full Text]  
  • Mizuguchi, G., Vassilev, A., Tsukiyama, T., Nakatani, Y., Wu, C. (2001). ATP-dependent Nucleosome Remodeling and Histone Hyperacetylation Synergistically Facilitate Transcription of Chromatin. J. Biol. Chem. 276: 14773-14783 [Abstract] [Full Text]  
  • Nasrin, N., Ogg, S., Cahill, C. M., Biggs, W., Nui, S., Dore, J., Calvo, D., Shi, Y., Ruvkun, G., Alexander-Bridges, M. C. (2000). DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in HepG2 cells. Proc. Natl. Acad. Sci. USA 97: 10412-10417 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»