This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mahmood, R.
Right arrow Articles by Ray, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mahmood, R.
Right arrow Articles by Ray, D. S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 1999, p. 6174-6182, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of cis and trans Elements Involved in the Cell Cycle Regulation of Multiple Genes in Crithidia fasciculata

Riaz Mahmood, Jane C. Hines, and Dan S. Ray*

Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California 90095-1570

Received 31 March 1999/Returned for modification 9 June 1999/Accepted 15 June 1999

Transcripts of several DNA replication genes, including the RPA1 and TOP2 genes, encoding the large subunit of nuclear replication protein A and the kinetoplast topoisomerase II, accumulate periodically during the cell cycle in the trypanosomatid Crithidia fasciculata. An octamer consensus sequence, CAUAGAAG, present in the 5' untranslated regions (UTR) of these mRNAs is required for periodic accumulation of the TOP2 and RPA1 transcripts and also for binding of a nuclear factor(s) to the 5' UTR RNAs of these genes. We show here that insertion of multiple (six) copies of this octamer sequence (6× octamer) into the 5' UTR of a reporter gene confers periodic accumulation on its transcript. Competition experiments and UV cross-linking studies show that the 6× octamer RNA and TOP2 5' UTR RNA bind to the same nuclear factor(s). Single-nucleotide substitutions in the 6× octamer that abolish the RNA gel shift also prevent cyclic accumulation of the reporter gene transcript. A protein termed cycling element binding protein, purified by affinity chromatography using 6× octamer RNA as a ligand, binds to RNAs containing wild-type octamers and not to those with mutant octamers. These results define a small sequence element in C. fasciculata mRNAs required for their cell cycle regulation and report the identification and purification of a putative regulatory protein that binds specifically to these elements.

* Corresponding author. Mailing address: Molecular Biology Institute, 611 Charles E. Young Dr. East, UCLA, Los Angeles, CA 90095-1570. Phone: (310) 825-4178. Fax: (310) 206-7286. E-mail: danray{at}mbi.ucla.edu.

Molecular and Cellular Biology, September 1999, p. 6174-6182, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Li, Y., Sun, Y., Hines, J. C., Ray, D. S. (2007). Identification of New Kinetoplast DNA Replication Proteins in Trypanosomatids Based on Predicted S-Phase Expression and Mitochondrial Targeting. Eukaryot Cell 6: 2303-2310 [Abstract] [Full Text]  
  • Sinha, K. M., Hines, J. C., Ray, D. S. (2006). Cell Cycle-Dependent Localization and Properties of a Second Mitochondrial DNA Ligase in Crithidia fasciculata. Eukaryot Cell 5: 54-61 [Abstract] [Full Text]  
  • Folgueira, C., Quijada, L., Soto, M., Abanades, D. R., Alonso, C., Requena, J. M. (2005). The Translational Efficiencies of the Two Leishmania infantum HSP70 mRNAs, Differing in Their 3'-Untranslated Regions, Are Affected by Shifts in the Temperature of Growth through Different Mechanisms. J. Biol. Chem. 280: 35172-35183 [Abstract] [Full Text]  
  • Zick, A., Onn, I., Bezalel, R., Margalit, H., Shlomai, J. (2005). Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements. Nucleic Acids Res 33: 4235-4242 [Abstract] [Full Text]  
  • WEBB, H., BURNS, R., KIMBLIN, N., ELLIS, L., CARRINGTON, M. (2005). A novel strategy to identify the location of necessary and sufficient cis-acting regulatory mRNA elements in trypanosomes. RNA 11: 1108-1116 [Abstract] [Full Text]  
  • Mittra, B., Ray, D. S. (2004). Presence of a Poly(A) Binding Protein and Two Proteins with Cell Cycle-Dependent Phosphorylation in Crithidia fasciculata mRNA Cycling Sequence Binding Protein II. Eukaryot Cell 3: 1185-1197 [Abstract] [Full Text]  
  • Mishra, K. K., Holzer, T. R., Moore, L. L., LeBowitz, J. H. (2003). A Negative Regulatory Element Controls mRNA Abundance of the Leishmania mexicana Paraflagellar Rod Gene PFR2. Eukaryot Cell 2: 1009-1017 [Abstract] [Full Text]  
  • Hanke, T., Ramiro, M. J., Trigueros, S., Roca, J., Larraga, V. (2003). Cloning, functional analysis and post-transcriptional regulation of a type II DNA topoisomerase from Leishmania infantum. A new potential target for anti-parasite drugs. Nucleic Acids Res 31: 4917-4928 [Abstract] [Full Text]  
  • Avliyakulov, N. K., Hines, J. C., Ray, D. S. (2003). Sequence Elements in both the Intergenic Space and the 3' Untranslated Region of the Crithidia fasciculata KAP3 Gene Are Required for Cell Cycle Regulation of KAP3 mRNA. Eukaryot Cell 2: 671-677 [Abstract] [Full Text]  
  • Mittra, B., Sinha, K. M., Hines, J. C., Ray, D. S. (2003). Presence of Multiple mRNA Cycling Sequence Element-binding Proteins in Crithidia fasciculata. J. Biol. Chem. 278: 26564-26571 [Abstract] [Full Text]  
  • Gaziova, I., Lukes, J. (2003). Mitochondrial and Nuclear Localization of Topoisomerase II in the Flagellate Bodo saltans (Kinetoplastida), a Species with Non-catenated Kinetoplast DNA. J. Biol. Chem. 278: 10900-10907 [Abstract] [Full Text]  
  • Milone, J., Wilusz, J., Bellofatto, V. (2002). Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts. Nucleic Acids Res 30: 4040-4050 [Abstract] [Full Text]  
  • Mahmood, R., Mittra, B., Hines, J. C., Ray, D. S. (2001). Characterization of the Crithidia fasciculata mRNA Cycling Sequence Binding Proteins. Mol. Cell. Biol. 21: 4453-4459 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»