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Molecular and Cellular Biology, May 2000, p. 3608-3615, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Roles for Non-TATA Core Promoter Sequences in Transcription and Factor Binding

Branden S. Wolner and Jay D. Gralla*

Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095-1569

Received 31 January 2000/Accepted 22 February 2000

Sequence blocks within the core region were swapped among RNA polymerase II promoters to explore effects on transcription in vitro. The pair of blocks flanking TATA strongly influenced general transcription, with an additional effect on promoter activation. These flanking elements induced a change in the ratio of activated to basal transcription, whereas swapping TATA and initiator sequences only altered general transcription levels. Swapping the flanking blocks influenced binding by general transcription factors TBP and TFIIB. The results suggest that the architecture of the extended core sequence is important in determining promoter-specific effects on both general transcription levels and the tightness of regulation.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, P.O. Box 951569, Los Angeles, CA 90095-1569. Phone: (310) 825-1620. Fax: (310) 267-2302. E-mail: gralla{at}ewald.mbi.ucla.edu.

Molecular and Cellular Biology, May 2000, p. 3608-3615, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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