This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vainberg, I. E.
Right arrow Articles by Rosbash, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vainberg, I. E.
Right arrow Articles by Rosbash, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2000, p. 3996-4005, Vol. 20, No. 11
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Nuclear Export of Heat Shock and Non-Heat-Shock mRNA Occurs via Similar Pathways

Irina E. Vainberg, Ken Dower, and Michael Rosbash*

Department of Biology, Howard Hughes Medical Institute, MS008 Brandeis University, Waltham, Massachusetts 02454

Received 8 November 1999/Returned for modification 20 December 1999/Accepted 8 March 2000

Several studies of the yeast Saccharomyces cerevisiae support differential regulation of heat shock mRNA (hs mRNA) and non-hs mRNA nuclear export during stress. These include the finding that hs mRNA export at 42°C is inhibited in the absence of the nucleoporinlike protein Rip1p (also called Nup42p) (C. A. Saavedra, C. M. Hammell, C. V. Heath, and C. N. Cole, Genes Dev. 11:2845-2856, 1997; F. Stutz, J. Kantor, D. Zhang, T. McCarthy, M. Neville, and M. Rosbash, Genes Dev. 11:2857-2868, 1997). However, the results reported in this paper provide little evidence for selective non-hs mRNA retention or selective hs mRNA export under heat shock conditions. First, we do not detect a block to non-hs mRNA export at 42°C in a wild-type strain. Second, hs mRNA export appears to be mediated by the Ran system and several other factors previously reported to be important for general mRNA export. Third, the export of non-hs mRNA as well as hs mRNA is inhibited in the absence of Rip1p at 42°C. As a corollary, we find no evidence for cis-acting hs mRNA sequences that promote transport during heat shock. Taken together, our data suggest that a shift to 42°C in the absence of Rip1p impacts a late stage of transport affecting most if not all mRNA.

* Corresponding author. Mailing address: Department of Biology, Howard Hughes Medical Institute, MS008 Brandeis University, Waltham, MA 02454. Phone: (781) 736-3161. Fax: (781) 736-3164. E-mail address: rosbash{at}brandeis.edu.

Molecular and Cellular Biology, June 2000, p. 3996-4005, Vol. 20, No. 11
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pardo, M., Nurse, P. (2005). The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast. J. Cell Sci. 118: 1705-1714 [Abstract] [Full Text]  
  • Izawa, S., Takemura, R., Miki, T., Inoue, Y. (2005). Characterization of the Export of Bulk Poly(A)+ mRNA in Saccharomyces cerevisiae during the Wine-Making Process. Appl. Environ. Microbiol. 71: 2179-2182 [Abstract] [Full Text]  
  • KUPERWASSER, N., BROGNA, S., DOWER, K., ROSBASH, M. (2004). Nonsense-mediated decay does not occur within the yeast nucleus. RNA 10: 1907-1915 [Abstract] [Full Text]  
  • Belanger, K. D., Simmons, L. A., Roth, J. K., VanderPloeg, K. A., Lichten, L. B., Fahrenkrog, B. (2004). The Karyopherin Msn5/Kap142 Requires Nup82 for Nuclear Export and Performs a Function Distinct from Translocation in RPA Protein Import. J. Biol. Chem. 279: 43530-43539 [Abstract] [Full Text]  
  • Izawa, S., Takemura, R., Inoue, Y. (2004). Gle2p Is Essential to Induce Adaptation of the Export of Bulk Poly(A)+ mRNA to Heat Shock in Saccharomyces cerevisiae. J. Biol. Chem. 279: 35469-35478 [Abstract] [Full Text]  
  • Takemura, R., Inoue, Y., Izawa, S. (2004). Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock. J. Cell Sci. 117: 4189-4197 [Abstract] [Full Text]  
  • CHEKANOVA, J. A., BELOSTOTSKY, D. A. (2003). Evidence that poly(A) binding protein has an evolutionarily conserved function in facilitating mRNA biogenesis and export. RNA 9: 1476-1490 [Abstract] [Full Text]  
  • THOMSEN, R., LIBRI, D., BOULAY, J., ROSBASH, M., JENSEN, T. H. (2003). Localization of nuclear retained mRNAs in Saccharomyces cerevisiae. RNA 9: 1049-1057 [Abstract] [Full Text]  
  • Farago, M., Nahari, T., Hammel, C., Cole, C. N., Choder, M. (2003). Rpb4p, a Subunit of RNA Polymerase II, Mediates mRNA Export during Stress. Mol. Biol. Cell 14: 2744-2755 [Abstract] [Full Text]  
  • Gatfield, D., Izaurralde, E. (2002). REF1/Aly and the additional exon junction complex proteins are dispensable for nuclear mRNA export. JCB 159: 579-588 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»