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Molecular and Cellular Biology, June 2000, p. 4405-4410, Vol. 20, No. 12
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning and Functional Characterization of the Early-Lymphocyte-Specific Pb99 Gene

Barry P. Sleckman,1,dagger Wasif N. Khan,1,Dagger Wanping Xu,2 Craig H. Bassing,1 Barbara A. Malynn,1 Neal G. Copeland,3 Christiana G. Bardon,1 Timo M. Breit,1 Laurie Davidson,1 Eugene M. Oltz,1,Dagger Nancy A. Jenkins,3 Jeffrey E. Berman,2 and Frederick W. Alt1,*

Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 021151; Medical Biotechnology Center, Department of Microbiology and Immunology, and Program in Molecular and Cell Biology, University of Maryland, Baltimore, Maryland 212012; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 217023

Received 8 March 2000/Accepted 20 March 2000

The Pb99 gene is specifically expressed in pre-B cells and thymocytes and not in mature B and T cells or nonlymphoid tissues, implying that it may function in early lymphoid development. We have previously described the cloning of an incomplete cDNA for Pb99. Here we report the isolation of full-length cDNAs and genomic clones for the murine Pb99 gene and the mapping of its location to mouse chromosome 8. Sequence analyses of different Pb99 cDNA clones suggest that there may be at least three forms of the Pb99 protein generated by differential processing of the Pb99 transcript. The cDNA with the longest open reading frame encodes a putative protein that has seven hydrophobic domains similar to those of seven membrane-spanning proteins, such as the classical G protein-coupled receptors. To directly address the role of the Pb99 protein in lymphoid development, Pb99-deficient mice were generated by gene targeting, and lymphocyte development in these mice was analyzed.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Children's Hospital, Longwood Ave., Boston, MA 02115. Phone: (617) 355-7290. Fax: (617) 355-3432. E-mail: alt{at}rascal.med.harvard.edu.

dagger Present address: Washington University School of Medicine, Department of Pathology and Immunology, St. Louis, MO 63110.

Dagger Present address: Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232-2363.

Molecular and Cellular Biology, June 2000, p. 4405-4410, Vol. 20, No. 12
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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