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Molecular and Cellular Biology, August 2000, p. 5939-5946, Vol. 20, No. 16
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Two Proteins Pat1p (Mrt1p) and Spb8p Interact In Vivo, Are Required for mRNA Decay, and Are Functionally Linked to Pab1p

Claire Bonnerot,1 Ronald Boeck,2 and Bruno Lapeyre1,*

Centre de Recherche de Biochimie Macromoléculaire du CNRS, 34293 Montpellier, France,1 and Centre Médical Universitaire, Université de Genève, CH-1211 Geneva, Switzerland2

Received 27 January 2000/Returned for modification 28 March 2000/Accepted 15 May 2000

We report here the characterization of a bypass suppressor of pab1Delta which leads to a fourfold stabilization of the unstable MFA2 mRNA. Cloning of the wild-type gene for that suppressor reveals that it is identical to PAT1 (YCR077c), a gene whose product was reported to interact with Top2p. PAT1 is not an essential gene, but its deletion leads to a thermosensitive phenotype. Further analysis has shown that PAT1 is allelic with mrt1-3, a mutation previously reported to affect decapping and to bypass suppress pab1Delta , as is also the case for dcp1, spb8, and mrt3. Coimmunoprecipitation experiments show that Pat1p is associated with Spb8p. On sucrose gradients, the two proteins cosediment with fractions containing the polysomes. In the absence of Pat1p, however, Spb8p no longer cofractionates with the polysomes, while the removal of Spb8p leads to a sharp decrease in the level of Pat1p. Our results suggest that some of the factors involved in mRNA degradation could be associated with the mRNA that is still being translated, awaiting a specific signal to commit the mRNA to the degradation pathway.

* Corresponding author. Mailing address: Centre de Recherche de Biochimie Macromoléculaire du CNRS, 1919 Route de Mende, 34293 Montpellier Cedex 5, France. Phone: 33 467 61 36 80. Fax: 33 467 04 02 31. E-mail: lapeyre{at}crbm.cnrs-mop.fr or bonnerot{at}crbm.cnrs-mop.fr.

Molecular and Cellular Biology, August 2000, p. 5939-5946, Vol. 20, No. 16
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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