This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shan, X.
Right arrow Articles by Wange, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shan, X.
Right arrow Articles by Wange, R. L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2000, p. 6945-6957, Vol. 20, No. 18
0270-7306/00/$04.00+0

Deficiency of PTEN in Jurkat T Cells Causes Constitutive Localization of Itk to the Plasma Membrane and Hyperresponsiveness to CD3 Stimulation

Xiaochuan Shan,1 Michael J. Czar,2 Stephen C. Bunnell,3 Pinghu Liu,1 Yusen Liu,1 Pamela L. Schwartzberg,2 and Ronald L. Wange1,*

Laboratory of Biological Chemistry, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224-6825,1 and Genetic Disease Research Branch, National Human Genome Research Institute,2 and Laboratory of Cellular and Molecular Biology, National Cancer Institute,3 National Institutes of Health, Bethesda, Maryland 20892

Received 16 March 2000/Returned for modification 24 April 2000/Accepted 16 June 2000

Pleckstrin homology (PH) domain binding to D3-phosphorylated phosphatidylinositides (PI) provides a reversible means of recruiting proteins to the plasma membrane, with the resultant change in subcellular localization playing a key role in the activation of multiple intracellular signaling pathways. Previously we found that the T-cell-specific PH domain-containing kinase Itk is constitutively membrane associated in Jurkat T cells. This distribution was unexpected given that the closely related B-cell kinase, Btk, is almost exclusively cytosolic. In addition to constitutive membrane association of Itk, unstimulated JTAg T cells also exhibited constitutive phosphorylation of Akt on Ser-473, an indication of elevated basal levels of the phosphatidylinositol 3-kinase (PI3K) products PI-3,4-P2 and PI-3,4,5-P3 in the plasma membrane. Here we describe a defect in expression of the D3 phosphoinositide phosphatase, PTEN, in Jurkat and JTAg T cells that leads to unregulated PH domain interactions with the plasma membrane. Inhibition of D3 phosphorylation by PI3K inhibitors, or by expression of PTEN, blocked constitutive phosphorylation of Akt on Ser-473 and caused Itk to redistribute to the cytosol. The PTEN-deficient cells were also hyperresponsive to T-cell receptor (TCR) stimulation, as measured by Itk kinase activity, tyrosine phosphorylation of phospholipase C-gamma 1, and activation of Erk compared to those in PTEN-replete cells. These data support the idea that PH domain-mediated association with the plasma membrane is required for Itk activation, provide evidence for a negative regulatory role of PTEN in TCR stimulation, and suggest that signaling models based on results from Jurkat T-cell lines may underestimate the role of PI3K in TCR signaling.

* Corresponding author. Mailing address: Gerontology Research Center, MSC-12, 5600 Nathan Shock Dr., Baltimore, MD 21224-6825. Phone: (410) 558-8054. Fax: (410) 558-8107. E-mail: wanger{at}grc.nia.nih.gov.

Molecular and Cellular Biology, September 2000, p. 6945-6957, Vol. 20, No. 18
0270-7306/00/$04.00+0


This article has been cited by other articles:

  • Rigby, S., Huang, Y., Streubel, B., Chott, A., Du, M.-Q., Turner, S. D., Bacon, C. M. (2009). The Lymphoma-associated Fusion Tyrosine Kinase ITK-SYK Requires Pleckstrin Homology Domain-mediated Membrane Localization for Activation and Cellular Transformation. J. Biol. Chem. 284: 26871-26881 [Abstract] [Full Text]  
  • Evenou, J.-P., Wagner, J., Zenke, G., Brinkmann, V., Wagner, K., Kovarik, J., Welzenbach, K. A., Weitz-Schmidt, G., Guntermann, C., Towbin, H., Cottens, S., Kaminski, S., Letschka, T., Lutz-Nicoladoni, C., Gruber, T., Hermann-Kleiter, N., Thuille, N., Baier, G. (2009). The Potent Protein Kinase C-Selective Inhibitor AEB071 (Sotrastaurin) Represents a New Class of Immunosuppressive Agents Affecting Early T-Cell Activation. J. Pharmacol. Exp. Ther. 330: 792-801 [Abstract] [Full Text]  
  • Fernandes, A. F., Bian, Q., Jiang, J.-K., Thomas, C. J., Taylor, A., Pereira, P., Shang, F. (2009). Proteasome Inactivation Promotes p38 Mitogen-activated Protein Kinase-dependent Phosphatidylinositol 3-kinase Activation and Increases Interleukin-8 Production in Retinal Pigment Epithelial Cells. Mol. Biol. Cell 20: 3690-3699 [Abstract] [Full Text]  
  • Gruber, T., Hermann-Kleiter, N., Hinterleitner, R., Fresser, F., Schneider, R., Gastl, G., Penninger, J. M., Baier, G. (2009). PKC-{theta} Modulates the Strength of T Cell Responses by Targeting Cbl-b for Ubiquitination and Degradation. Sci Signal 2: ra30-ra30 [Abstract] [Full Text]  
  • Rao, S. S., O'Neil, J., Liberator, C. D., Hardwick, J. S., Dai, X., Zhang, T., Tyminski, E., Yuan, J., Kohl, N. E., Richon, V. M., Van der Ploeg, L. H.T., Carroll, P. M., Draetta, G. F., Look, A. T., Strack, P. R., Winter, C. G. (2009). Inhibition of NOTCH Signaling by Gamma Secretase Inhibitor Engages the RB Pathway and Elicits Cell Cycle Exit in T-Cell Acute Lymphoblastic Leukemia Cells. Cancer Res. 69: 3060-3068 [Abstract] [Full Text]  
  • Sakamoto, K., Iwasaki, K., Sugiyama, H., Tsuji, Y. (2009). Role of the Tumor Suppressor PTEN in Antioxidant Responsive Element-mediated Transcription and Associated Histone Modifications. Mol. Biol. Cell 20: 1606-1617 [Abstract] [Full Text]  
  • Takeda, K., Harada, Y., Watanabe, R., Inutake, Y., Ogawa, S., Onuki, K., Kagaya, S., Tanabe, K., Kishimoto, H., Abe, R. (2008). CD28 stimulation triggers NF-{kappa}B activation through the CARMA1-PKC{theta}-Grb2/Gads axis. Int Immunol 20: 1507-1515 [Abstract] [Full Text]  
  • Johnson, C. M., Chichili, G. R., Rodgers, W. (2008). Compartmentalization of Phosphatidylinositol 4,5-Bisphosphate Signaling Evidenced Using Targeted Phosphatases. J. Biol. Chem. 283: 29920-29928 [Abstract] [Full Text]  
  • Fabre, S., Carrette, F., Chen, J., Lang, V., Semichon, M., Denoyelle, C., Lazar, V., Cagnard, N., Dubart-Kupperschmitt, A., Mangeney, M., Fruman, D. A., Bismuth, G. (2008). FOXO1 Regulates L-Selectin and a Network of Human T Cell Homing Molecules Downstream of Phosphatidylinositol 3-Kinase. J. Immunol. 181: 2980-2989 [Abstract] [Full Text]  
  • Harris, S. J., Parry, R. V., Westwick, J., Ward, S. G. (2008). Phosphoinositide Lipid Phosphatases: Natural Regulators of Phosphoinositide 3-Kinase Signaling in T Lymphocytes. J. Biol. Chem. 283: 2465-2469 [Abstract] [Full Text]  
  • Su, C.-C., Lin, Y.-P., Cheng, Y.-J., Huang, J.-Y., Chuang, W.-J., Shan, Y.-S., Yang, B.-C. (2007). Phosphatidylinositol 3-Kinase/Akt Activation by Integrin-Tumor Matrix Interaction Suppresses Fas-Mediated Apoptosis in T Cells. J. Immunol. 179: 4589-4597 [Abstract] [Full Text]  
  • Kishimoto, H., Ohteki, T., Yajima, N., Kawahara, K., Natsui, M., Kawarasaki, S., Hamada, K., Horie, Y., Kubo, Y., Arase, S., Taniguchi, M., Vanhaesebroeck, B., Mak, T. W., Nakano, T., Koyasu, S., Sasaki, T., Suzuki, A. (2007). The Pten/PI3K pathway governs the homeostasis of V{alpha}14iNKT cells. Blood 109: 3316-3324 [Abstract] [Full Text]  
  • Beach, D., Gonen, R., Bogin, Y., Reischl, I. G., Yablonski, D. (2007). Dual Role of SLP-76 in Mediating T Cell Receptor-induced Activation of Phospholipase C-{gamma}1. J. Biol. Chem. 282: 2937-2946 [Abstract] [Full Text]  
  • Dennehy, K. M., Elias, F., Na, S.-Y., Fischer, K.-D., Hunig, T., Luhder, F. (2007). Mitogenic CD28 Signals Require the Exchange Factor Vav1 to Enhance TCR Signaling at the SLP-76-Vav-Itk Signalosome. J. Immunol. 178: 1363-1371 [Abstract] [Full Text]  
  • Kim, J. S., Eom, J. I., Cheong, J.-W., Choi, A. J., Lee, J. K., Yang, W. I., Min, Y. H. (2007). Protein Kinase CK2{alpha} as an Unfavorable Prognostic Marker and Novel Therapeutic Target in Acute Myeloid Leukemia. Clin. Cancer Res. 13: 1019-1028 [Abstract] [Full Text]  
  • Schade, A. E., Powers, J. J., Wlodarski, M. W., Maciejewski, J. P. (2006). Phosphatidylinositol-3-phosphate kinase pathway activation protects leukemic large granular lymphocytes from undergoing homeostatic apoptosis. Blood 107: 4834-4840 [Abstract] [Full Text]  
  • Chemnitz, J. M., Lanfranco, A. R., Braunstein, I., Riley, J. L. (2006). B and T Lymphocyte Attenuator-Mediated Signal Transduction Provides a Potent Inhibitory Signal to Primary Human CD4 T Cells That Can Be Initiated by Multiple Phosphotyrosine Motifs.. J. Immunol. 176: 6603-6614 [Abstract] [Full Text]  
  • Khan, I. H., Mendoza, S., Rhyne, P., Ziman, M., Tuscano, J., Eisinger, D., Kung, H.-J., Luciw, P. A. (2006). Multiplex Analysis of Intracellular Signaling Pathways in Lymphoid Cells by Microbead Suspension Arrays. Mol. Cell. Proteomics 5: 758-768 [Abstract] [Full Text]  
  • Narayan, P., Holt, B., Tosti, R., Kane, L. P. (2006). CARMA1 Is Required for Akt-Mediated NF-{kappa}B Activation in T Cells.. Mol. Cell. Biol. 26: 2327-2336 [Abstract] [Full Text]  
  • Kim, J.-E., White, F. M. (2006). Quantitative analysis of phosphotyrosine signaling networks triggered by CD3 and CD28 costimulation in jurkat cells.. J. Immunol. 176: 2833-2843 [Abstract] [Full Text]  
  • Chemnitz, J. M., Driesen, J., Classen, S., Riley, J. L., Debey, S., Beyer, M., Popov, A., Zander, T., Schultze, J. L. (2006). Prostaglandin E2 Impairs CD4+ T Cell Activation by Inhibition of lck: Implications in Hodgkin's Lymphoma. Cancer Res. 66: 1114-1122 [Abstract] [Full Text]  
  • Gao, P., Wange, R. L., Zhang, N., Oppenheim, J. J., Howard, O. M. Z. (2005). Negative regulation of CXCR4-mediated chemotaxis by the lipid phosphatase activity of tumor suppressor PTEN. Blood 106: 2619-2626 [Abstract] [Full Text]  
  • Mehenni, H., Lin-Marq, N., Buchet-Poyau, K., Reymond, A., Collart, M. A., Picard, D., Antonarakis, S. E. (2005). LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes. Hum Mol Genet 14: 2209-2219 [Abstract] [Full Text]  
  • Huang, Y., Norton, D. D., Precht, P., Martindale, J. L., Burkhardt, J. K., Wange, R. L. (2005). Deficiency of ADAP/Fyb/SLAP-130 Destabilizes SKAP55 in Jurkat T Cells. J. Biol. Chem. 280: 23576-23583 [Abstract] [Full Text]  
  • Kane, L. P., Watkins, S. C. (2005). Dynamic Regulation of Tec Kinase Localization in Membrane-proximal Vesicles of a T Cell Clone Revealed by Total Internal Reflection Fluorescence and Confocal Microscopy. J. Biol. Chem. 280: 21949-21954 [Abstract] [Full Text]  
  • Gonen, R., Beach, D., Ainey, C., Yablonski, D. (2005). T Cell Receptor-induced Activation of Phospholipase C-{gamma}1 Depends on a Sequence-independent Function of the P-I Region of SLP-76. J. Biol. Chem. 280: 8364-8370 [Abstract] [Full Text]  
  • Lundholt, B. K., Linde, V., Loechel, F., Pedersen, H.-C., Moller, S., Praestegaard, M., Mikkelsen, I., Scudder, K., Bjorn, S. P., Heide, M., Arkhammar, P. O. G., Terry, R., Nielsen, S. J. (2005). Identification of Akt Pathway Inhibitors Using Redistribution Screening on the FLIPR and the IN Cell 3000 Analyzer. J Biomol Screen 10: 20-29 [Abstract]  
  • Dombroski, D., Houghtling, R. A., Labno, C. M., Precht, P., Takesono, A., Caplen, N. J., Billadeau, D. D., Wange, R. L., Burkhardt, J. K., Schwartzberg, P. L. (2005). Kinase-Independent Functions for Itk in TCR-Induced Regulation of Vav and the Actin Cytoskeleton. J. Immunol. 174: 1385-1392 [Abstract] [Full Text]  
  • Wu, L. X., La Rose, J., Chen, L., Neale, C., Mak, T., Okkenhaug, K., Wange, R., Rottapel, R. (2005). CD28 Regulates the Translation of Bcl-xL via the Phosphatidylinositol 3-Kinase/Mammalian Target of Rapamycin Pathway. J. Immunol. 174: 180-194 [Abstract] [Full Text]  
  • Tomlinson, M. G., Heath, V. L., Turck, C. W., Watson, S. P., Weiss, A. (2004). SHIP Family Inositol Phosphatases Interact with and Negatively Regulate the Tec Tyrosine Kinase. J. Biol. Chem. 279: 55089-55096 [Abstract] [Full Text]  
  • Mao, J.-H., To, M. D., Perez-Losada, J., Wu, D., Del Rosario, R., Balmain, A. (2004). Mutually exclusive mutations of the Pten and ras pathways in skin tumor progression. Genes Dev. 18: 1800-1805 [Abstract] [Full Text]  
  • Chemnitz, J. M., Parry, R. V., Nichols, K. E., June, C. H., Riley, J. L. (2004). SHP-1 and SHP-2 Associate with Immunoreceptor Tyrosine-Based Switch Motif of Programmed Death 1 upon Primary Human T Cell Stimulation, but Only Receptor Ligation Prevents T Cell Activation. J. Immunol. 173: 945-954 [Abstract] [Full Text]  
  • Huang, Y., Wange, R. L. (2004). T Cell Receptor Signaling: Beyond Complex Complexes. J. Biol. Chem. 279: 28827-28830 [Full Text]  
  • von Essen, M., Bonefeld, C. M., Siersma, V., Rasmussen, A. B., Lauritsen, J. P. H., Nielsen, B. L., Geisler, C. (2004). Constitutive and Ligand-Induced TCR Degradation. J. Immunol. 173: 384-393 [Abstract] [Full Text]  
  • Mattioli, I., Sebald, A., Bucher, C., Charles, R.-P., Nakano, H., Doi, T., Kracht, M., Schmitz, M. L. (2004). Transient and Selective NF-{kappa}B p65 Serine 536 Phosphorylation Induced by T Cell Costimulation Is Mediated by I{kappa}B Kinase {beta} and Controls the Kinetics of p65 Nuclear Import. J. Immunol. 172: 6336-6344 [Abstract] [Full Text]  
  • Gomez-Mouton, C., Lacalle, R. A., Mira, E., Jimenez-Baranda, S., Barber, D. F., Carrera, A. C., Martinez-A., C., Manes, S. (2004). Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis. JCB 164: 759-768 [Abstract] [Full Text]  
  • Pardo, J., Buferne, M., Martinez-Lorenzo, M.-J., Naval, J., Schmitt-Verhulst, A.-M., Boyer, C., Anel, A. (2003). Differential implication of protein kinase C isoforms in cytotoxic T lymphocyte degranulation and TCR-induced Fas ligand expression. Int Immunol 15: 1441-1450 [Abstract] [Full Text]  
  • Ge, B., Li, O., Wilder, P., Rizzino, A., McKeithan, T. W. (2003). NF-{kappa}B Regulates BCL3 Transcription in T Lymphocytes Through an Intronic Enhancer. J. Immunol. 171: 4210-4218 [Abstract] [Full Text]  
  • Wilcox, H. M., Berg, L. J. (2003). Itk Phosphorylation Sites Are Required for Functional Activity in Primary T Cells. J. Biol. Chem. 278: 37112-37121 [Abstract] [Full Text]  
  • Cipres, A., Carrasco, S., Merino, E., Diaz, E., Krishna, U. M., Falck, J. R., Martinez-A, C., Merida, I. (2003). Regulation of Diacylglycerol Kinase {alpha} by Phosphoinositide 3-Kinase Lipid Products. J. Biol. Chem. 278: 35629-35635 [Abstract] [Full Text]  
  • Salazar-Fontana, L. I., Barr, V., Samelson, L. E., Bierer, B. E. (2003). CD28 Engagement Promotes Actin Polymerization Through the Activation of the Small Rho GTPase Cdc42 in Human T Cells. J. Immunol. 171: 2225-2232 [Abstract] [Full Text]  
  • Jordan, S., Rodgers, W. (2003). T Cell Glycolipid-Enriched Membrane Domains Are Constitutively Assembled as Membrane Patches That Translocate to Immune Synapses. J. Immunol. 171: 78-87 [Abstract] [Full Text]  
  • Parry, R. V., Rumbley, C. A., Vandenberghe, L. H., June, C. H., Riley, J. L. (2003). CD28 and Inducible Costimulatory Protein Src Homology 2 Binding Domains Show Distinct Regulation of Phosphatidylinositol 3-Kinase, Bcl-xL, and IL-2 Expression in Primary Human CD4 T Lymphocytes. J. Immunol. 171: 166-174 [Abstract] [Full Text]  
  • Bunnell, S. C., Barr, V. A., Fuller, C. L., Samelson, L. E. (2003). High-Resolution Multicolor Imaging of Dynamic Signaling Complexes in T Cells Stimulated by Planar Substrates. Sci Signal 2003: pl8-pl8 [Abstract] [Full Text]  
  • Suzuki, A., Itami, S., Ohishi, M., Hamada, K., Inoue, T., Komazawa, N., Senoo, H., Sasaki, T., Takeda, J., Manabe, M., Mak, T. W., Nakano, T. (2003). Keratinocyte-specific Pten Deficiency Results in Epidermal Hyperplasia, Accelerated Hair Follicle Morphogenesis and Tumor Formation. Cancer Res. 63: 674-681 [Abstract] [Full Text]  
  • Yin, L., Schneider, H., Rudd, C. E. (2003). Short cytoplasmic SDYMNM segment of CD28 is sufficient to convert CTLA-4 to a positive signaling receptor. J. Leukoc. Biol. 73: 178-182 [Abstract] [Full Text]  
  • Sheridan, C. M., Heist, E. K., Beals, C. R., Crabtree, G. R., Gardner, P. (2002). Protein Kinase A Negatively Modulates the Nuclear Accumulation of NF-ATc1 by Priming for Subsequent Phosphorylation by Glycogen Synthase Kinase-3. J. Biol. Chem. 277: 48664-48676 [Abstract] [Full Text]  
  • Attema, J. L., Reeves, R., Murray, V., Levichkin, I., Temple, M. D., Tremethick, D. J., Shannon, M. F. (2002). The Human IL-2 Gene Promoter Can Assemble a Positioned Nucleosome That Becomes Remodeled Upon T Cell Activation. J. Immunol. 169: 2466-2476 [Abstract] [Full Text]  
  • Kane, L. P., Mollenauer, M. N., Xu, Z., Turck, C. W., Weiss, A. (2002). Akt-Dependent Phosphorylation Specifically Regulates Cot Induction of NF-{kappa}B-Dependent Transcription. Mol. Cell. Biol. 22: 5962-5974 [Abstract] [Full Text]  
  • Jones, R. G., Elford, A. R., Parsons, M. J., Wu, L., Krawczyk, C. M., Yeh, W.-C., Hakem, R., Rottapel, R., Woodgett, J. R., Ohashi, P. S. (2002). CD28-dependent Activation of Protein Kinase B/Akt Blocks Fas-mediated Apoptosis by Preventing Death-inducing Signaling Complex Assembly. JEM 196: 335-348 [Abstract] [Full Text]  
  • Xu, Z., Stokoe, D., Kane, L. P., Weiss, A. (2002). The Inducible Expression of the Tumor Suppressor Gene PTEN Promotes Apoptosis and Decreases Cell Size by Inhibiting the PI3K/Akt Pathway in Jurkat T Cells. Cell Growth Differ. 13: 285-296 [Abstract] [Full Text]  
  • Klem, J., Verrett, P. C., Kumar, V., Schatzle, J. D. (2002). 2B4 Is Constitutively Associated with Linker for the Activation of T Cells in Glycolipid-Enriched Microdomains: Properties Required for 2B4 Lytic Function. J. Immunol. 169: 55-62 [Abstract] [Full Text]  
  • Birle, D., Bottini, N., Williams, S., Huynh, H., deBelle, I., Adamson, E., Mustelin, T. (2002). Negative Feedback Regulation of the Tumor Suppressor PTEN by Phosphoinositide-Induced Serine Phosphorylation. J. Immunol. 169: 286-291 [Abstract] [Full Text]  
  • Chirathaworn, C., Kohlmeier, J. E., Tibbetts, S. A., Rumsey, L. M., Chan, M. A., Benedict, S. H. (2002). Stimulation Through Intercellular Adhesion Molecule-1 Provides a Second Signal for T Cell Activation. J. Immunol. 168: 5530-5537 [Abstract] [Full Text]  
  • Appleman, L. J., van Puijenbroek, A. A. F. L., Shu, K. M., Nadler, L. M., Boussiotis, V. A. (2002). CD28 Costimulation Mediates Down-Regulation of p27kip1 and Cell Cycle Progression by Activation of the PI3K/PKB Signaling Pathway in Primary Human T Cells. J. Immunol. 168: 2729-2736 [Abstract] [Full Text]  
  • Yamazaki, T., Takeda, K., Gotoh, K., Takeshima, H., Akira, S., Kurosaki, T. (2002). Essential Immunoregulatory Role for BCAP in B Cell Development and Function. JEM 195: 535-545 [Abstract] [Full Text]  
  • Takesono, A., Finkelstein, L. D., Schwartzberg, P. L. (2002). Beyond calcium: new signaling pathways for Tec family kinases. J. Cell Sci. 115: 3039-3048 [Abstract] [Full Text]  
  • Shan, X., Balakir, R., Criado, G., Wood, J. S., Seminario, M.-C., Madrenas, J., Wange, R. L. (2001). ZAP-70-Independent Ca2+ Mobilization and Erk Activation in Jurkat T Cells in Response to T-Cell Antigen Receptor Ligation. Mol. Cell. Biol. 21: 7137-7149 [Abstract] [Full Text]  
  • Veri, M.-C., DeBell, K. E., Seminario, M.-C., DiBaldassarre, A., Reischl, I., Rawat, R., Graham, L., Noviello, C., Rellahan, B. L., Miscia, S., Wange, R. L., Bonvini, E. (2001). Membrane Raft-Dependent Regulation of Phospholipase C{gamma}-1 Activation in T Lymphocytes. Mol. Cell. Biol. 21: 6939-6950 [Abstract] [Full Text]  
  • Magnan, A., Di Bartolo, V., Mura, A.-M., Boyer, C., Richelme, M., Lin, Y.-L., Roure, A., Gillet, A., Arrieumerlou, C., Acuto, O., Malissen, B., Malissen, M. (2001). T Cell Development and T Cell Responses in Mice with Mutations Affecting Tyrosines 292 or 315 of the Zap-70 Protein Tyrosine Kinase. JEM 194: 491-506 [Abstract] [Full Text]  
  • Woods, M. L., Shimizu, Y. (2001). Signaling networks regulating {beta}1 integrin-mediated adhesion of T lymphocytes to extracellular matrix. J. Leukoc. Biol. 69: 874-880 [Abstract] [Full Text]  
  • Herndon, T. M., Shan, X. C., Tsokos, G. C., Wange, R. L. (2001). ZAP-70 and SLP-76 Regulate Protein Kinase C-{{theta}} and NF-{{kappa}}B Activation in Response to Engagement of CD3 and CD28. J. Immunol. 166: 5654-5664 [Abstract] [Full Text]  
  • Kunjibettu, S., Fuller-Espie, S., Carey, G. B., Spain, L. M. (2001). Conserved transmembrane tyrosine residues of the TCR {beta} chain are required for TCR expression and function in primary T cells and hybridomas. Int Immunol 13: 211-222 [Abstract] [Full Text]  
  • Cantrell, D. (2001). Phosphoinositide 3-kinase signalling pathways. J. Cell Sci. 114: 1439-1445 [Abstract]  
  • Mittelstadt, P. R., Ashwell, J. D. (2001). Inhibition of AP-1 by the Glucocorticoid-inducible Protein GILZ. J. Biol. Chem. 276: 29603-29610 [Abstract] [Full Text]  
  • Bauer, B., Krumbock, N., Fresser, F., Hochholdinger, F., Spitaler, M., Simm, A., Uberall, F., Schraven, B., Baier, G. (2001). Complex Formation and Cooperation of Protein Kinase Ctheta and Akt1/Protein Kinase Balpha in the NF-kappa B Transactivation Cascade in Jurkat T Cells. J. Biol. Chem. 276: 31627-31634 [Abstract] [Full Text]  
  • Yamasaki, S., Nishida, K., Hibi, M., Sakuma, M., Shiina, R., Takeuchi, A., Ohnishi, H., Hirano, T., Saito, T. (2001). Docking Protein Gab2 Is Phosphorylated by ZAP-70 and Negatively Regulates T Cell Receptor Signaling by Recruitment of Inhibitory Molecules. J. Biol. Chem. 276: 45175-45183 [Abstract] [Full Text]  
  • Zubiaur, M., Fernandez, O., Ferrero, E., Salmeron, J., Malissen, B., Malavasi, F., Sancho, J. (2002). CD38 Is Associated with Lipid Rafts and upon Receptor Stimulation Leads to Akt/Protein Kinase B and Erk Activation in the Absence of the CD3-zeta Immune Receptor Tyrosine-based Activation Motifs. J. Biol. Chem. 277: 13-22 [Abstract] [Full Text]  
  • Kang, H., Schneider, H., Rudd, C. E. (2002). Phosphatidylinositol 3-Kinase p85 Adaptor Function in T-cells. CO-STIMULATION AND REGULATION OF CYTOKINE TRANSCRIPTION INDEPENDENT OF ASSOCIATED p110. J. Biol. Chem. 277: 912-921 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»