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Molecular and Cellular Biology, September 2000, p. 7007-7012, Vol. 20, No. 18
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Absence of Nidogen 1 Does Not Affect Murine Basement Membrane Formation

Monzur Murshed,1 Neil Smyth,2 Nicolai Miosge,3 Jörg Karolat,2 Thomas Krieg,1 Mats Paulsson,2 and Roswitha Nischt1,*

Department of Dermatology1 and Institute for Biochemistry II,2 Medical Faculty, University of Cologne, D-50924 Cologne, and Center of Anatomy, Department of Histology, University of Göttingen, D-37075 Göttingen,3 Germany

Received 12 June 2000/Accepted 20 June 2000

Nidogen 1 is a highly conserved protein in mammals, Drosophila melanogaster, Caenorhabditis elegans, and ascidians and is found in all basement membranes. It has been proposed that nidogen 1 connects the laminin and collagen IV networks, so stabilizing the basement membrane, and integrates other proteins, including perlecan, into the basement membrane. To define the role of nidogen 1 in basement membranes in vivo, we produced a null mutation of the NID-1 gene in embryonic stem cells and used these to derive mouse lines. Homozygous animals produce neither nidogen 1 mRNA nor protein. Surprisingly, they show no overt abnormalities and are fertile, their basement membrane structures appearing normal. Nidogen 2 staining is increased in certain basement membranes, where it is normally only found in scant amounts. This occurs by either redistribution from other extracellular matrices or unmasking of nidogen 2 epitopes, as its production does not appear to be upregulated. The results show that nidogen 1 is not required for basement membrane formation or maintenance.

* Corresponding author. Mailing address: Department of Dermatology, University of Cologne, 50924 Cologne, Germany. Phone: 49-221-478-5472. Fax: 49-221-478-5949. E-mail: roswitha.nischt{at}uni-koeln.de.

Molecular and Cellular Biology, September 2000, p. 7007-7012, Vol. 20, No. 18
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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