Previous Article | Next Article 
Molecular and Cellular Biology, October 2000, p. 7220-7229, Vol. 20, No. 19
Institute of Biochemistry and
Biophysics, 02-106 Warsaw,1 and Faculty
of Biotechnology, University of Gdansk, 80-822 Gdansk,3 Poland; Biozentrum, University
of Basel, CH-4056 Basel, Switzerland2;
and MPI Enzymology of Protein Folding, Biozentrum Halle,
D-06120 Halle, Germany4
Received 15 May 2000/Accepted 29 June 2000
Nam9p is a protein of the mitochondrial ribosome. The
respiration-deficient Saccharomyces cerevisiae strain
MB43-nam9-1 expresses Nam9-1p containing the point mutation
S82L. Respiratory deficiency correlates with a decrease in the steady
level of some mitochondrially encoded proteins and the complete lack of
mitochondrially encoded cytochrome oxidase subunit 2 (Cox2). De novo
synthesis of Cox2 in MB43-nam9-1 is unaffected, indicating
that newly synthesized Cox2 is rapidly degraded. Respiratory deficiency
of MB43-nam9-1 is overcome by transient overexpression of
HSP104, by deletion of HSP104, by transient
exposure to guanidine hydrochloride, and by expression of the
C-terminal portion of Sup35, indicating an involvement of the yeast
prion [PSI+]. Respiratory deficiency of
MB43-nam9-1 can be reinduced by transfer of cytosol from
S. cerevisiae that harbors
[PSI+]. We conclude that nam9-1
causes respiratory deficiency only in combination with the cytosolic
prion [PSI+], presenting the first example of
a synthetic effect between cytosolic [PSI+]
and a mutant mitochondrial protein.
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Prion-Dependent Switching between Respiratory
Competence and Deficiency in the Yeast nam9-1
Mutant
*
Corresponding author. Mailing address: MPI Enzymology
of Protein Folding, Biozentrum Halle, Weinbergweg 22, D-06120 Halle, Germany. Phone: 49-345-5522830. Fax: 49-345-5511972. E-mail for Sabine Rospert: rospert{at}enzyme-halle.mpg.de. E-mail for
Magdalena Boguta: magda{at}ibbrain.ibb.waw.pl.
Molecular and Cellular Biology, October 2000, p. 7220-7229, Vol. 20, No. 19
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Rakwalska, M., Rospert, S.
(2004). The Ribosome-Bound Chaperones RAC and Ssb1/2p Are Required for Accurate Translation in Saccharomyces cerevisiae. Mol. Cell. Biol.
24: 9186-9197
[Abstract] [Full Text] -
Towpik, J., Chacinska, A., Ciesla, M., Ginalski, K., Boguta, M.
(2004). Mutations in the Yeast MRF1 Gene Encoding Mitochondrial Release Factor Inhibit Translation on Mitochondrial Ribosomes. J. Biol. Chem.
279: 14096-14103
[Abstract] [Full Text] -
Kryndushkin, D. S., Alexandrov, I. M., Ter-Avanesyan, M. D., Kushnirov, V. V.
(2003). Yeast [PSI+] Prion Aggregates Are Formed by Small Sup35 Polymers Fragmented by Hsp104. J. Biol. Chem.
278: 49636-49643
[Abstract] [Full Text] -
Dziembowski, A., Piwowarski, J., Hoser, R., Minczuk, M., Dmochowska, A., Siep, M., van der Spek, H., Grivell, L., Stepien, P. P.
(2003). The Yeast Mitochondrial Degradosome. ITS COMPOSITION, INTERPLAY BETWEEN RNA HELICASE AND RNase ACTIVITIES AND THE ROLE IN MITOCHONDRIAL RNA METABOLISM. J. Biol. Chem.
278: 1603-1611
[Abstract] [Full Text] -
Stribinskis, V., Gao, G.-J., Ellis, S. R., Martin, N. C.
(2001). Rpm2, the Protein Subunit of Mitochondrial RNase P in Saccharomyces cerevisiae, Also Has a Role in the Translation of Mitochondrially Encoded Subunits of Cytochrome c Oxidase. Genetics
158: 573-585
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»