This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishimura, S.
Right arrow Articles by Yamamoto, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishimura, S.
Right arrow Articles by Yamamoto, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2000, p. 713-723, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A GATA Box in the GATA-1 Gene Hematopoietic Enhancer Is a Critical Element in the Network of GATA Factors and Sites That Regulate This Gene

Shigeko Nishimura,1 Satoru Takahashi,1 Takashi Kuroha,1 Naruyoshi Suwabe,1 Toshiro Nagasawa,2 Cecelia Trainor,3 and Masayuki Yamamoto1,*

Center for Tsukuba Advanced Research Alliance and Institutes of Basic Medical Sciences1 and Clinical Medicine,2 University of Tsukuba, Tsukuba 305-8577, Japan, and Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 208923

Received 5 April 1999/Returned for modification 18 May 1999/Accepted 18 October 1999

A region located at kbp -3.9 to -2.6 5' to the first hematopoietic exon of the GATA-1 gene is necessary to recapitulate gene expression in both the primitive and definitive erythroid lineages. In transfection analyses, this region activated reporter gene expression from an artificial promoter in a position- and orientation-independent manner, indicating that the region functions as the GATA-1 gene hematopoietic enhancer (G1HE). However, when analyzed in transgenic embryos in vivo, G1HE activity was orientation dependent and also required the presence of the endogenous GATA-1 gene hematopoietic promoter. To define the boundaries of G1HE, a series of deletion constructs were prepared and tested in transfection and transgenic mice analyses. We show that G1HE contains a 149-bp core region which is critical for GATA-1 gene expression in both primitive and definitive erythroid cells but that expression in megakaryocytes requires the core plus additional sequences from G1HE. This core region contains one GATA, one GAT, and two E boxes. Mutational analyses revealed that only the GATA box is critical for gene-regulatory activity. Importantly, G1HE was active in SCL-/- embryos. These results thus demonstrate the presence of a critical network of GATA factors and GATA binding sites that controls the expression of this gene.

* Corresponding author. Mailing address: Center for TARA, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8577, Japan. Phone: 81-298-53-6158. Fax: 81-298-53-7318. E-mail: masi{at}tara.tsukuba.ac.jp.

Molecular and Cellular Biology, January 2000, p. 713-723, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ohneda, K., Ohmori, S., Ishijima, Y., Nakano, M., Yamamoto, M. (2009). Characterization of a Functional ZBP-89 Binding Site That Mediates Gata1 Gene Expression during Hematopoietic Development. J. Biol. Chem. 284: 30187-30199 [Abstract] [Full Text]  
  • Shimizu, R., Kobayashi, E., Engel, J. D., Yamamoto, M. (2009). Induction of hyperproliferative fetal megakaryopoiesis by an N-terminally truncated GATA1 mutant. GENES CELLS 14: 1119-1131 [Abstract] [Full Text]  
  • Marques-Garcia, F., Ferrandiz, N., Fernandez-Alonso, R., Gonzalez-Cano, L., Herreros-Villanueva, M., Rosa-Garrido, M., Fernandez-Garcia, B., Vaque, J. P., Marques, M. M., Alonso, M. E., Segovia, J. C., Leon, J., Marin, M. C. (2009). p73 Plays a Role in Erythroid Differentiation through GATA1 Induction. J. Biol. Chem. 284: 21139-21156 [Abstract] [Full Text]  
  • Chou, S. T., Khandros, E., Bailey, L. C., Nichols, K. E., Vakoc, C. R., Yao, Y., Huang, Z., Crispino, J. D., Hardison, R. C., Blobel, G. A., Weiss, M. J. (2009). Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate. Blood 114: 983-994 [Abstract] [Full Text]  
  • Tripic, T., Deng, W., Cheng, Y., Zhang, Y., Vakoc, C. R., Gregory, G. D., Hardison, R. C., Blobel, G. A. (2009). SCL and associated proteins distinguish active from repressive GATA transcription factor complexes. Blood 113: 2191-2201 [Abstract] [Full Text]  
  • Suzuki, M., Moriguchi, T., Ohneda, K., Yamamoto, M. (2009). Differential Contribution of the Gata1 Gene Hematopoietic Enhancer to Erythroid Differentiation. Mol. Cell. Biol. 29: 1163-1175 [Abstract] [Full Text]  
  • Hamlett, I., Draper, J., Strouboulis, J., Iborra, F., Porcher, C., Vyas, P. (2008). Characterization of megakaryocyte GATA1-interacting proteins: the corepressor ETO2 and GATA1 interact to regulate terminal megakaryocyte maturation. Blood 112: 2738-2749 [Abstract] [Full Text]  
  • Tanabe, O., Shen, Y., Liu, Q., Campbell, A. D., Kuroha, T., Yamamoto, M., Engel, J. D. (2007). The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription. Genes Dev. 21: 2832-2844 [Abstract] [Full Text]  
  • Ogilvy, S., Ferreira, R., Piltz, S. G., Bowen, J. M., Gottgens, B., Green, A. R. (2007). The SCL +40 Enhancer Targets the Midbrain Together with Primitive and Definitive Hematopoiesis and Is Regulated by SCL and GATA Proteins. Mol. Cell. Biol. 27: 7206-7219 [Abstract] [Full Text]  
  • Kobayashi, M., Yamamoto, M. (2007). Regulation of GATA1 Gene Expression. J Biochem 142: 1-10 [Abstract] [Full Text]  
  • Nagano, M., Yamashita, T., Hamada, H., Ohneda, K., Kimura, K.-i., Nakagawa, T., Shibuya, M., Yoshikawa, H., Ohneda, O. (2007). Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood 110: 151-160 [Abstract] [Full Text]  
  • Shimizu, R., Trainor, C. D., Nishikawa, K., Kobayashi, M., Ohneda, K., Yamamoto, M. (2007). GATA-1 Self-association Controls Erythroid Development in Vivo. J. Biol. Chem. 282: 15862-15871 [Abstract] [Full Text]  
  • Ema, M., Yokomizo, T., Wakamatsu, A., Terunuma, T., Yamamoto, M., Takahashi, S. (2006). Primitive erythropoiesis from mesodermal precursors expressing VE-cadherin, PECAM-1, Tie2, endoglin, and CD34 in the mouse embryo. Blood 108: 4018-4024 [Abstract] [Full Text]  
  • Nakajima, O., Okano, S., Harada, H., Kusaka, T., Gao, X., Hosoya, T., Suzuki, N., Takahashi, S., Yamamoto, M. (2006). Transgenic rescue of erythroid 5-aminolevulinate synthase-deficient mice results in the formation of ring sideroblasts and siderocytes. GENES CELLS 11: 685-700 [Abstract] [Full Text]  
  • Bose, F., Fugazza, C., Casalgrandi, M., Capelli, A., Cunningham, J. M., Zhao, Q., Jane, S. M., Ottolenghi, S., Ronchi, A. (2006). Functional Interaction of CP2 with GATA-1 in the Regulation of Erythroid Promoters. Mol. Cell. Biol. 26: 3942-3954 [Abstract] [Full Text]  
  • Guyot, B., Murai, K., Fujiwara, Y., Valverde-Garduno, V., Hammett, M., Wells, S., Dear, N., Orkin, S. H., Porcher, C., Vyas, P. (2006). Characterization of a Megakaryocyte-specific Enhancer of the Key Hemopoietic Transcription Factor GATA1. J. Biol. Chem. 281: 13733-13742 [Abstract] [Full Text]  
  • Zheng, J., Kitajima, K., Sakai, E., Kimura, T., Minegishi, N., Yamamoto, M., Nakano, T. (2006). Differential effects of GATA-1 on proliferation and differentiation of erythroid lineage cells. Blood 107: 520-527 [Abstract] [Full Text]  
  • Patterson, L. J., Gering, M., Patient, R. (2005). Scl is required for dorsal aorta as well as blood formation in zebrafish embryos. Blood 105: 3502-3511 [Abstract] [Full Text]  
  • Nakano, M., Ohneda, K., Yamamoto-Mukai, H., Shimizu, R., Ohneda, O., Ohmura, S., Suzuki, M., Tsukamoto, S., Yanagawa, T., Yoshida, H., Takakuwa, Y., Yamamoto, M. (2005). Transgenic over-expression of GATA-1 mutant lacking N-finger domain causes hemolytic syndrome in mouse erythroid cells. GENES CELLS 10: 47-62 [Abstract] [Full Text]  
  • Shimizu, R., Kuroha, T., Ohneda, O., Pan, X., Ohneda, K., Takahashi, S., Philipsen, S., Yamamoto, M. (2004). Leukemogenesis Caused by Incapacitated GATA-1 Function. Mol. Cell. Biol. 24: 10814-10825 [Abstract] [Full Text]  
  • Khandekar, M., Suzuki, N., Lewton, J., Yamamoto, M., Engel, J. D. (2004). Multiple, Distant Gata2 Enhancers Specify Temporally and Tissue-Specific Patterning in the Developing Urogenital System. Mol. Cell. Biol. 24: 10263-10276 [Abstract] [Full Text]  
  • Valverde-Garduno, V., Guyot, B., Anguita, E., Hamlett, I., Porcher, C., Vyas, P. (2004). Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 loci: implications for cis-element identification. Blood 104: 3106-3116 [Abstract] [Full Text]  
  • Shimizu, R., Ohneda, K., Engel, J. D., Trainor, C. D., Yamamoto, M. (2004). Transgenic rescue of GATA-1-deficient mice with GATA-1 lacking a FOG-1 association site phenocopies patients with X-linked thrombocytopenia. Blood 103: 2560-2567 [Abstract] [Full Text]  
  • Testa, A., Lotti, F., Cairns, L., Grande, A., Ottolenghi, S., Ferrari, G., Ronchi, A. (2004). Deletion of a Negatively Acting Sequence in a Chimeric GATA-1 Enhancer-Long Terminal Repeat Greatly Increases Retrovirally Mediated Erythroid Expression. J. Biol. Chem. 279: 10523-10531 [Abstract] [Full Text]  
  • Gottgens, B., Broccardo, C., Sanchez, M.-J., Deveaux, S., Murphy, G., Gothert, J. R., Kotsopoulou, E., Kinston, S., Delaney, L., Piltz, S., Barton, L. M., Knezevic, K., Erber, W. N., Begley, C. G., Frampton, J., Green, A. R. (2004). The scl +18/19 Stem Cell Enhancer Is Not Required for Hematopoiesis: Identification of a 5' Bifunctional Hematopoietic-Endothelial Enhancer Bound by Fli-1 and Elf-1. Mol. Cell. Biol. 24: 1870-1883 [Abstract] [Full Text]  
  • Sun-Wada, G.-H., Kamei, Y., Wada, Y., Futai, M. (2004). Regulatory Elements Directing Gut Expression of the GATA6 Gene during Mouse Early Development. J Biochem 135: 165-169 [Abstract] [Full Text]  
  • Cairns, L. A., Moroni, E., Levantini, E., Giorgetti, A., Klinger, F. G., Ronzoni, S., Tatangelo, L., Tiveron, C., De Felici, M., Dolci, S., Magli, M. C., Giglioni, B., Ottolenghi, S. (2003). Kit regulatory elements required for expression in developing hematopoietic and germ cell lineages. Blood 102: 3954-3962 [Abstract] [Full Text]  
  • Nishikawa, K., Kobayashi, M., Masumi, A., Lyons, S. E., Weinstein, B. M., Liu, P. P., Yamamoto, M. (2003). Self-Association of Gata1 Enhances Transcriptional Activity In Vivo in Zebra Fish Embryos. Mol. Cell. Biol. 23: 8295-8305 [Abstract] [Full Text]  
  • Suzuki, N., Suwabe, N., Ohneda, O., Obara, N., Imagawa, S., Pan, X., Motohashi, H., Yamamoto, M. (2003). Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels. Blood 102: 3575-3583 [Abstract] [Full Text]  
  • Xu, Z., Huang, S., Chang, L.-S., Agulnick, A. D., Brandt, S. J. (2003). Identification of a TAL1 Target Gene Reveals a Positive Role for the LIM Domain-Binding Protein Ldb1 in Erythroid Gene Expression and Differentiation. Mol. Cell. Biol. 23: 7585-7599 [Abstract] [Full Text]  
  • Swamynathan, S. K., Piatigorsky, J. (2002). Orientation-dependent Influence of an Intergenic Enhancer on the Promoter Activity of the Divergently Transcribed Mouse Shsp/alpha B-crystallin and Mkbp/HspB2 Genes. J. Biol. Chem. 277: 49700-49706 [Abstract] [Full Text]  
  • Kwak, M.-K., Itoh, K., Yamamoto, M., Kensler, T. W. (2002). Enhanced Expression of the Transcription Factor Nrf2 by Cancer Chemopreventive Agents: Role of Antioxidant Response Element-Like Sequences in the nrf2 Promoter. Mol. Cell. Biol. 22: 2883-2892 [Abstract] [Full Text]  
  • Kobayashi, M., Nishikawa, K., Yamamoto, M. (2001). Hematopoietic regulatory domain of gata1 gene is positively regulated by GATA1 protein in zebrafish embryos. Development 128: 2341-2350 [Abstract] [Full Text]  
  • Ranganath, S., Murphy, K. M. (2001). Structure and Specificity of GATA Proteins in Th2 Development. Mol. Cell. Biol. 21: 2716-2725 [Abstract] [Full Text]  
  • Wei, W., Brennan, M. D. (2000). Polarity of transcriptional enhancement revealed by an insulator element. Proc. Natl. Acad. Sci. USA 10.1073/pnas.011529598v1 [Abstract] [Full Text]  
  • Seshasayee, D., Geiger, J. N., Gaines, P., Wojchowski, D. M. (2000). Intron 1 Elements Promote Erythroid-specific GATA-1 Gene Expression. J. Biol. Chem. 275: 22969-22977 [Abstract] [Full Text]  
  • Wei, W., Brennan, M. D. (2000). Polarity of transcriptional enhancement revealed by an insulator element. Proc. Natl. Acad. Sci. USA 97: 14518-14523 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»