This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chong, L. D.
Right arrow Articles by Daar, I. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chong, L. D.
Right arrow Articles by Daar, I. O.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2000, p. 724-734, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Fibroblast Growth Factor Receptor-Mediated Rescue of x-Ephrin B1-Induced Cell Dissociation in Xenopus Embryos

Lisa D. Chong,1 Eui Kyun Park,1 Erin Latimer,2 Robert Friesel,3 and Ira O. Daar1,*

Basic Research Laboratory, National Cancer Institute,1 and Scientific Applications International Corp.,2 Frederick Cancer Research & Development Center, Frederick, Maryland 21702, and Center for Molecular Medicine, Maine Medical Center Research Institute, South Portland, Maine 041063

Received 6 August 1999/Returned for modification 21 September 1999/Accepted 14 October 1999

The Eph family of receptor tyrosine kinases and their membrane-bound ligands, the ephrins, have been implicated in regulating cell adhesion and migration during development by mediating cell-to-cell signaling events. Genetic evidence suggests that ephrins may transduce signals and become tyrosine phosphorylated during embryogenesis. However, the induction and functional significance of ephrin phosphorylation is not yet clear. Here, we report that when we used ectopically expressed proteins, we found that an activated fibroblast growth factor (FGF) receptor associated with and induced the phosphorylation of ephrin B1 on tyrosine. Moreover, this phosphorylation reduced the ability of overexpressed ephrin B1 to reduce cell adhesion. In addition, we identified a region in the cytoplasmic tail of ephrin B1 that is critical for interaction with the FGF receptor; we also report FGF-induced phosphorylation of ephrins in a neural tissue. This is the first demonstration of communication between the FGF receptor family and the Eph ligand family and implicates cross talk between these two cell surface molecules in regulating cell adhesion.

* Corresponding author. Mailing address: Building 567, Room 228, National Cancer Institute---Frederick Cancer Research & Development Center, Frederick, MD 21702. Phone: (301) 846-1667. Fax: (301) 846-6641. E-mail: daar{at}ncifcrf.gov.

Molecular and Cellular Biology, January 2000, p. 724-734, Vol. 20, No. 2
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Salvucci, O., Maric, D., Economopoulou, M., Sakakibara, S., Merlin, S., Follenzi, A., Tosato, G. (2009). EphrinB reverse signaling contributes to endothelial and mural cell assembly into vascular structures. Blood 114: 1707-1716 [Abstract] [Full Text]  
  • Bush, J. O., Soriano, P. (2009). Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Genes Dev. 23: 1586-1599 [Abstract] [Full Text]  
  • Lee, H.-S., Mood, K., Battu, G., Ji, Y. J., Singh, A., Daar, I. O. (2009). Fibroblast Growth Factor Receptor-induced Phosphorylation of EphrinB1 Modulates Its Interaction with Dishevelled. Mol. Biol. Cell 20: 124-133 [Abstract] [Full Text]  
  • Poliakov, A., Cotrina, M. L., Pasini, A., Wilkinson, D. G. (2008). Regulation of EphB2 activation and cell repulsion by feedback control of the MAPK pathway. JCB 183: 933-947 [Abstract] [Full Text]  
  • Arvanitis, D., Davy, A. (2008). Eph/ephrin signaling: networks. Genes Dev. 22: 416-429 [Abstract] [Full Text]  
  • Jiang, G., Freywald, T., Webster, J., Kozan, D., Geyer, R., DeCoteau, J., Narendran, A., Freywald, A. (2008). In Human Leukemia Cells Ephrin-B-Induced Invasive Activity Is Supported by Lck and Is Associated with Reassembling of Lipid Raft Signaling Complexes. Mol Cancer Res 6: 291-305 [Abstract] [Full Text]  
  • Bong, Y.-S., Lee, H.-S., Carim-Todd, L., Mood, K., Nishanian, T. G., Tessarollo, L., Daar, I. O. (2007). ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3. Proc. Natl. Acad. Sci. USA 104: 17305-17310 [Abstract] [Full Text]  
  • Hayashi, S.-i., Asahara, T., Masuda, H., Isner, J. M., Losordo, D. W. (2005). Functional Ephrin-B2 Expression for Promotive Interaction Between Arterial and Venous Vessels in Postnatal Neovascularization. Circulation 111: 2210-2218 [Abstract] [Full Text]  
  • Goldshmit, Y., Galea, M. P., Wise, G., Bartlett, P. F., Turnley, A. M. (2004). Axonal Regeneration and Lack of Astrocytic Gliosis in EphA4-Deficient Mice. J. Neurosci. 24: 10064-10073 [Abstract] [Full Text]  
  • Twigg, S. R. F., Kan, R., Babbs, C., Bochukova, E. G., Robertson, S. P., Wall, S. A., Morriss-Kay, G. M., Wilkie, A. O. M. (2004). Mutations of ephrin-B1 (EFNB1), a marker of tissue boundary formation, cause craniofrontonasal syndrome. Proc. Natl. Acad. Sci. USA 101: 8652-8657 [Abstract] [Full Text]  
  • Park, E. K., Warner, N., Bong, Y.-S., Stapleton, D., Maeda, R., Pawson, T., Daar, I. O. (2004). Ectopic EphA4 Receptor Induces Posterior Protrusions via FGF Signaling in Xenopus Embryos. Mol. Biol. Cell 15: 1647-1655 [Abstract] [Full Text]  
  • Murai, K. K., Pasquale, E. B. (2003). `Eph'ective signaling: forward, reverse and crosstalk. J. Cell Sci. 116: 2823-2832 [Abstract] [Full Text]  
  • Kovalenko, D., Yang, X., Nadeau, R. J., Harkins, L. K., Friesel, R. (2003). Sef Inhibits Fibroblast Growth Factor Signaling by Inhibiting FGFR1 Tyrosine Phosphorylation and Subsequent ERK Activation. J. Biol. Chem. 278: 14087-14091 [Abstract] [Full Text]  
  • Glickman, N. S., Kimmel, C. B., Jones, M. A., Adams, R. J. (2003). Shaping the zebrafish notochord. Development 130: 873-887 [Abstract] [Full Text]  
  • Park, E. K., Warner, N., Mood, K., Pawson, T., Daar, I. O. (2002). Low-Molecular-Weight Protein Tyrosine Phosphatase Is a Positive Component of the Fibroblast Growth Factor Receptor Signaling Pathway. Mol. Cell. Biol. 22: 3404-3414 [Abstract] [Full Text]  
  • Maeda, R., Ishimura, A., Mood, K., Park, E. K., Buchberg, A. M., Daar, I. O. (2002). Xpbx1b and Xmeis1b play a collaborative role in hindbrain and neural crest gene expression in Xenopus embryos. Proc. Natl. Acad. Sci. USA 99: 5448-5453 [Abstract] [Full Text]  
  • Kalo, M. S., Yu, H.-H., Pasquale, E. B. (2001). In Vivo Tyrosine Phosphorylation Sites of Activated Ephrin-B1 and EphB2 from Neural Tissue. J. Biol. Chem. 276: 38940-38948 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»