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Molecular and Cellular Biology, October 2000, p. 7463-7479, Vol. 20, No. 20
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cooperative Assembly of an hnRNP Complex Induced by a Tissue-Specific Homolog of Polypyrimidine Tract Binding Protein

Vadim Markovtsov,1 Julia M. Nikolic,2 Joseph A. Goldman,1 Christoph W. Turck,3 Min-Yuan Chou,2 and Douglas L. Black1,2,*

Department of Microbiology and Molecular Genetics1 and Howard Hughes Medical Institute,2 University of California, Los Angeles, Los Angeles, California 90095, and Howard Hughes Medical Institute, Department of Medicine and Cardiovascular Research Institute, University of California, San Francisco, California 941433

Received 13 June 2000/Returned for modification 16 July 2000/Accepted 20 July 2000

Splicing of the c-src N1 exon in neuronal cells depends in part on an intronic cluster of RNA regulatory elements called the downstream control sequence (DCS). Using site-specific cross-linking, RNA gel shift, and DCS RNA affinity chromatography assays, we characterized the binding of several proteins to specific sites along the DCS RNA. Heterogeneous nuclear ribonucleoprotein (hnRNP) H, polypyrimidine tract binding protein (PTB), and KH-type splicing-regulatory protein (KSRP) each bind to distinct elements within this sequence. We also identified a new 60-kDa tissue-specific protein that binds to the CUCUCU splicing repressor element of the DCS RNA. This protein was purified, partially sequenced, and cloned. The new protein (neurally enriched homolog of PTB [nPTB]) is highly homologous to PTB. Unlike PTB, nPTB is enriched in the brain and in some neural cell lines. Although similar in sequence, nPTB and PTB show significant differences in their properties. nPTB binds more stably to the DCS RNA than PTB does but is a weaker repressor of splicing in vitro. nPTB also greatly enhances the binding of two other proteins, hnRNP H and KSRP, to the DCS RNA. These experiments identify specific cooperative interactions between the proteins that assemble onto an intricate splicing-regulatory sequence and show how this hnRNP assembly is altered in different cell types by incorporating different but highly related proteins.

* Corresponding author. Mailing address: 5-748 MRL, Box 951662, 675 Charles E. Young Dr. South, Los Angeles, CA 90095-1662. Phone: (310) 794-7644. Fax: (310) 206-8623. E-mail: DougB{at}microbio.lifesci.ucla.edu.

Molecular and Cellular Biology, October 2000, p. 7463-7479, Vol. 20, No. 20
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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