This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Douziech, M.
Right arrow Articles by Coulombe, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Douziech, M.
Right arrow Articles by Coulombe, B.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 2000, p. 8168-8177, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mechanism of Promoter Melting by the Xeroderma Pigmentosum Complementation Group B Helicase of Transcription Factor IIH Revealed by Protein-DNA Photo-Cross-Linking

Maxime Douziech,1 Frédéric Coin,2 Jean-Marc Chipoulet,2 Yoko Arai,3 Yoshiaki Ohkuma,3 Jean-Marc Egly,2 and Benoit Coulombe1,*

Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Québec J1K 2R1, Canada1; Institut de Génétique et de Biologie Moléculaire et Cellulaire, UPR 6520 (CNRS), Unité 184 (INSERM), Illkirch Cédex, CU de Strasbourg, France2; and Institute for Molecular and Cellular Biology, Institute for Molecular and Cellular Biology and Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan3

Received 23 June 2000/Returned for modification 18 July 2000/Accepted 26 July 2000

The p89/xeroderma pigmentosum complementation group B (XPB) ATPase-helicase of transcription factor IIH (TFIIH) is essential for promoter melting prior to transcription initiation by RNA polymerase II (RNAPII). By studying the topological organization of the initiation complex using site-specific protein-DNA photo-cross-linking, we have shown that p89/XPB makes promoter contacts both upstream and downstream of the initiation site. The upstream contact, which is in the region where promoter melting occurs (positions -9 to +2), requires tight DNA wrapping around RNAPII. The addition of hydrolyzable ATP tethers the template strand at positions -5 and +1 to RNAPII subunits. A mutation in p89/XPB found in a xeroderma pigmentosum patient impairs the ability of TFIIH to associate correctly with the complex and thereby melt promoter DNA. A model for open complex formation is proposed.

* Corresponding author. Mailing address: Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Québec J1K 2R1, Canada. Phone: (819) 821-8000, ext. 1092. Fax: (819) 821-7083. E-mail: b.coulom{at}courrier.usherb.ca.

Molecular and Cellular Biology, November 2000, p. 8168-8177, Vol. 20, No. 21
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tanaka, A., Watanabe, T., Iida, Y., Hanaoka, F., Ohkuma, Y. (2009). Central forkhead domain of human TFIIE{beta} plays a primary role in binding double-stranded DNA at transcription initiation. GENES CELLS 14: 395-405 [Abstract] [Full Text]  
  • Hirose, Y., Ohkuma, Y. (2007). Phosphorylation of the C-terminal Domain of RNA Polymerase II Plays Central Roles in the Integrated Events of Eucaryotic Gene Expression. J Biochem 141: 601-608 [Abstract] [Full Text]  
  • Trinh, V., Langelier, M.-F., Archambault, J., Coulombe, B. (2006). Structural Perspective on Mutations Affecting the Function of Multisubunit RNA Polymerases. Microbiol. Mol. Biol. Rev. 70: 12-36 [Abstract] [Full Text]  
  • O'Gorman, W., Thomas, B., Kwek, K. Y., Furger, A., Akoulitchev, A. (2005). Analysis of U1 Small Nuclear RNA Interaction with Cyclin H. J. Biol. Chem. 280: 36920-36925 [Abstract] [Full Text]  
  • Okuda, M., Tanaka, A., Hanaoka, F., Ohkuma, Y., Nishimura, Y. (2005). Structural Insights into the Asymmetric Effects of Zinc-Ligand Cysteine Mutations in the Novel Zinc Ribbon Domain of Human TFIIE{alpha} for Transcription. J Biochem 138: 443-449 [Abstract] [Full Text]  
  • Lin, Y. C., Gralla, J. D. (2005). Stimulation of the XPB ATP-dependent helicase by the beta subunit of TFIIE. Nucleic Acids Res 33: 3072-3081 [Abstract] [Full Text]  
  • Langelier, M.-F., Baali, D., Trinh, V., Greenblatt, J., Archambault, J., Coulombe, B. (2005). The highly conserved glutamic acid 791 of Rpb2 is involved in the binding of NTP and Mg(B) in the active center of human RNA polymerase II. Nucleic Acids Res 33: 2629-2639 [Abstract] [Full Text]  
  • Hayashi, K., Watanabe, T., Tanaka, A., Furumoto, T., Sato-Tsuchiya, C., Kimura, M., Yokoi, M., Ishihama, A., Hanaoka, F., Ohkuma, Y. (2005). Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation. GENES CELLS 10: 207-224 [Abstract] [Full Text]  
  • Okuda, M., Tanaka, A., Arai, Y., Satoh, M., Okamura, H., Nagadoi, A., Hanaoka, F., Ohkuma, Y., Nishimura, Y. (2004). A Novel Zinc Finger Structure in the Large Subunit of Human General Transcription Factor TFIIE. J. Biol. Chem. 279: 51395-51403 [Abstract] [Full Text]  
  • Jeronimo, C., Langelier, M.-F., Zeghouf, M., Cojocaru, M., Bergeron, D., Baali, D., Forget, D., Mnaimneh, S., Davierwala, A. P., Pootoolal, J., Chandy, M., Canadien, V., Beattie, B. K., Richards, D. P., Workman, J. L., Hughes, T. R., Greenblatt, J., Coulombe, B. (2004). RPAP1, a Novel Human RNA Polymerase II-Associated Protein Affinity Purified with Recombinant Wild-Type and Mutated Polymerase Subunits. Mol. Cell. Biol. 24: 7043-7058 [Abstract] [Full Text]  
  • Tapias, A., Auriol, J., Forget, D., Enzlin, J. H., Scharer, O. D., Coin, F., Coulombe, B., Egly, J.-M. (2004). Ordered Conformational Changes in Damaged DNA Induced by Nucleotide Excision Repair Factors. J. Biol. Chem. 279: 19074-19083 [Abstract] [Full Text]  
  • Forget, D., Langelier, M.-F., Therien, C., Trinh, V., Coulombe, B. (2004). Photo-Cross-Linking of a Purified Preinitiation Complex Reveals Central Roles for the RNA Polymerase II Mobile Clamp and TFIIE in Initiation Mechanisms. Mol. Cell. Biol. 24: 1122-1131 [Abstract] [Full Text]  
  • Kassavetis, G. A., Han, S., Naji, S., Geiduschek, E. P. (2003). The Role of Transcription Initiation Factor IIIB Subunits in Promoter Opening Probed by Photochemical Cross-linking. J. Biol. Chem. 278: 17912-17917 [Abstract] [Full Text]  
  • Watanabe, T., Hayashi, K., Tanaka, A., Furumoto, T., Hanaoka, F., Ohkuma, Y. (2003). The Carboxy Terminus of the Small Subunit of TFIIE Regulates the Transition from Transcription Initiation to Elongation by RNA Polymerase II. Mol. Cell. Biol. 23: 2914-2926 [Abstract] [Full Text]  
  • Wang, X., Spangler, L., Dvir, A. (2003). Promoter Escape by RNA Polymerase II. DOWNSTREAM PROMOTER DNA IS REQUIRED DURING MULTIPLE STEPS OF EARLY TRANSCRIPTION. J. Biol. Chem. 278: 10250-10256 [Abstract] [Full Text]  
  • Jawhari, A., Laine, J.-P., Dubaele, S., Lamour, V., Poterszman, A., Coin, F., Moras, D., Egly, J.-M. (2002). p52 Mediates XPB Function within the Transcription/Repair Factor TFIIH. J. Biol. Chem. 277: 31761-31767 [Abstract] [Full Text]  
  • Selzer, R. R., Nyaga, S., Tuo, J., May, A., Muftuoglu, M., Christiansen, M., Citterio, E., Brosh, R. M. Jr, Bohr, V. A. (2002). Differential requirement for the ATPase domain of the Cockayne syndrome group B gene in the processing of UV-induced DNA damage and 8-oxoguanine lesions in human cells. Nucleic Acids Res 30: 782-793 [Abstract] [Full Text]  
  • Langelier, M.-F., Forget, D., Rojas, A., Porlier, Y., Burton, Z. F., Coulombe, B. (2001). Structural and Functional Interactions of Transcription Factor (TF) IIA with TFIIE and TFIIF in Transcription Initiation by RNA Polymerase II. J. Biol. Chem. 276: 38652-38657 [Abstract] [Full Text]  
  • Spangler, L., Wang, X., Conaway, J. W., Conaway, R. C., Dvir, A. (2001). TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA. Proc. Natl. Acad. Sci. USA 10.1073/pnas.101004498v1 [Abstract] [Full Text]  
  • Tremeau-Bravard, A., Perez, C., Egly, J.-M. (2001). A Role of the C-terminal Part of p44 in the Promoter Escape Activity of Transcription Factor IIH. J. Biol. Chem. 276: 27693-27697 [Abstract] [Full Text]  
  • Spangler, L., Wang, X., Conaway, J. W., Conaway, R. C., Dvir, A. (2001). TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA. Proc. Natl. Acad. Sci. USA 98: 5544-5549 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»