This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shikama, N.
Right arrow Articles by La Thangue, N. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shikama, N.
Right arrow Articles by La Thangue, N. B.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2000, p. 8933-8943, Vol. 20, No. 23
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Functional Interaction between Nucleosome Assembly Proteins and p300/CREB-Binding Protein Family Coactivators

Noriko Shikama, Ho Man Chan, Marija Krstic-Demonacos, Linda Smith, Chang-Woo Lee, William Cairns, and Nicholas B. La Thangue*

Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom

Received 13 June 2000/Returned for modification 15 July 2000/Accepted 5 September 2000

The p300/CREB-binding protein (CBP) family of proteins consists of coactivators that influence the activity of a wide variety of transcription factors. Although the mechanisms that allow p300/CBP proteins to achieve transcriptional control are not clear, it is believed that the regulation of chromatin is an important aspect of the process. Here, we describe a new level of p300-dependent control mediated through the functional interaction between p300/CBP and members of the family of nucleosome assembly proteins (NAP), which includes NAP1, NAP2, and TAF1. We find that NAP proteins, which have previously been implicated in the regulation of transcription factor binding to chromatin, augment the activity of different p300 targets, including p53 and E2F, through a process that is likely to involve the physical interaction between p300 and NAP. NAP proteins can form oligomers, and the results show that NAP proteins can bind to both core histones and p300 coactivator proteins, perhaps in a multicomponent ternary complex. We also provide data in support of the idea that histones can influence the interaction between p300 and NAP protein. These results argue that NAP is a functionally important component of the p300 coactivator complex and suggest that NAP may serve as a point of integration between transcriptional coactivators and chromatin.

* Corresponding author. Mailing address: Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom. Phone: 44 141 330 5514. Fax: 44 141 330 5859. E-mail: nlathangue{at}bio.gla.ac.uk.

Molecular and Cellular Biology, December 2000, p. 8933-8943, Vol. 20, No. 23
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wang, S., Frappier, L. (2009). Nucleosome Assembly Proteins Bind to Epstein-Barr Virus Nuclear Antigen 1 and Affect Its Functions in DNA Replication and Transcriptional Activation. J. Virol. 83: 11704-11714 [Abstract] [Full Text]  
  • Selth, L. A., Lorch, Y., Ocampo-Hafalla, M. T., Mitter, R., Shales, M., Krogan, N. J., Kornberg, R. D., Svejstrup, J. Q. (2009). An Rtt109-Independent Role for Vps75 in Transcription-Associated Nucleosome Dynamics. Mol. Cell. Biol. 29: 4220-4234 [Abstract] [Full Text]  
  • Kaul, R., Murakami, M., Lan, K., Choudhuri, T., Robertson, E. S. (2009). EBNA3C Can Modulate the Activities of the Transcription Factor Necdin in Association with Metastasis Suppressor Protein Nm23-H1. J. Virol. 83: 4871-4883 [Abstract] [Full Text]  
  • Gazzar, M. E., Yoza, B. K., Chen, X., Garcia, B. A., Young, N. L., McCall, C. E. (2009). Chromatin-Specific Remodeling by HMGB1 and Linker Histone H1 Silences Proinflammatory Genes during Endotoxin Tolerance. Mol. Cell. Biol. 29: 1959-1971 [Abstract] [Full Text]  
  • Hou, T., Ray, S., Lee, C., Brasier, A. R. (2008). The STAT3 NH2-terminal Domain Stabilizes Enhanceosome Assembly by Interacting with the p300 Bromodomain. J. Biol. Chem. 283: 30725-30734 [Abstract] [Full Text]  
  • Sharma, N., Nyborg, J. K. (2008). The coactivators CBP/p300 and the histone chaperone NAP1 promote transcription-independent nucleosome eviction at the HTLV-1 promoter. Proc. Natl. Acad. Sci. USA 105: 7959-7963 [Abstract] [Full Text]  
  • Tachiwana, H., Osakabe, A., Kimura, H., Kurumizaka, H. (2008). Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro. Nucleic Acids Res 36: 2208-2218 [Abstract] [Full Text]  
  • Clark, J., Alvarez, D. F., Alexeyev, M., King, J. A. C., Huang, L., Yoder, M. C., Stevens, T. (2008). Regulatory role for nucleosome assembly protein-1 in the proliferative and vasculogenic phenotype of pulmonary endothelium. Am. J. Physiol. Lung Cell. Mol. Physiol. 294: L431-L439 [Abstract] [Full Text]  
  • Attia, M., Rachez, C., De Pauw, A., Avner, P., Rogner, U. C. (2007). Nap1l2 Promotes Histone Acetylation Activity during Neuronal Differentiation. Mol. Cell. Biol. 27: 6093-6102 [Abstract] [Full Text]  
  • Eckey, M., Hong, W., Papaioannou, M., Baniahmad, A. (2007). The Nucleosome Assembly Activity of NAP1 Is Enhanced by Alien. Mol. Cell. Biol. 27: 3557-3568 [Abstract] [Full Text]  
  • Zlatanova, J., Seebart, C., Tomschik, M. (2007). Nap1: taking a closer look at a juggler protein of extraordinary skills. FASEB J. 21: 1294-1310 [Abstract] [Full Text]  
  • Galichet, A., Gruissem, W. (2006). Developmentally Controlled Farnesylation Modulates AtNAP1;1 Function in Cell Proliferation and Cell Expansion during Arabidopsis Leaf Development. Plant Physiol. 142: 1412-1426 [Abstract] [Full Text]  
  • Zhu, Y., Dong, A., Meyer, D., Pichon, O., Renou, J.-P., Cao, K., Shen, W.-H. (2006). Arabidopsis NRP1 and NRP2 Encode Histone Chaperones and Are Required for Maintaining Postembryonic Root Growth. Plant Cell 18: 2879-2892 [Abstract] [Full Text]  
  • Friedeberg, C., Scarlett, G., McGeeghan, J., Abu-daya, A., Guille, M., Kneale, G. (2006). Identification of a structural and functional domain in xNAP1 involved in protein-protein interactions. Nucleic Acids Res 34: 4893-4899 [Abstract] [Full Text]  
  • Glanz, S., Bunse, A., Wimbert, A., Balczun, C., Kuck, U. (2006). A nucleosome assembly protein-like polypeptide binds to chloroplast group II intron RNA in Chlamydomonas reinhardtii. Nucleic Acids Res 34: 5337-5351 [Abstract] [Full Text]  
  • Kasper, L. H., Fukuyama, T., Biesen, M. A., Boussouar, F., Tong, C., de Pauw, A., Murray, P. J., van Deursen, J. M. A., Brindle, P. K. (2006). Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development. Mol. Cell. Biol. 26: 789-809 [Abstract] [Full Text]  
  • Kaul, R., Verma, S. C., Murakami, M., Lan, K., Choudhuri, T., Robertson, E. S. (2006). Epstein-Barr Virus Protein Can Upregulate Cyclo-Oxygenase-2 Expression through Association with the Suppressor of Metastasis Nm23-H1. J. Virol. 80: 1321-1331 [Abstract] [Full Text]  
  • Park, Y.-J., Luger, K. (2006). The structure of nucleosome assembly protein 1. Proc. Natl. Acad. Sci. USA 103: 1248-1253 [Abstract] [Full Text]  
  • Granja, A. G., Nogal, M. L., Hurtado, C., del Aguila, C., Carrascosa, A. L., Salas, M. L., Fresno, M., Revilla, Y. (2006). The Viral Protein A238L Inhibits TNF-{alpha} Expression through a CBP/p300 Transcriptional Coactivators Pathway. J. Immunol. 176: 451-462 [Abstract] [Full Text]  
  • Arlt, D., Huber, W., Liebel, U., Schmidt, C., Majety, M., Sauermann, M., Rosenfelder, H., Bechtel, S., Mehrle, A., Bannasch, D., Schupp, I., Seiler, M., Simpson, J. C., Hahne, F., Moosmayer, P., Ruschhaupt, M., Guilleaume, B., Wellenreuther, R., Pepperkok, R., Sultmann, H., Poustka, A., Wiemann, S. (2005). Functional Profiling: From Microarrays via Cell-Based Assays to Novel Tumor Relevant Modulators of the Cell Cycle. Cancer Res. 65: 7733-7742 [Abstract] [Full Text]  
  • Abu-Daya, A., Steer, W. M., Trollope, A. F., Friedeberg, C. E., Patient, R. K., Thorne, A. W., Guille, M. J. (2005). Zygotic nucleosome assembly protein-like 1 has a specific, non-cell autonomous role in hematopoiesis. Blood 106: 514-520 [Abstract] [Full Text]  
  • Dong, A., Liu, Z., Zhu, Y., Yu, F., Li, Z., Cao, K., Shen, W.-H. (2005). Interacting Proteins and Differences in Nuclear Transport Reveal Specific Functions for the NAP1 Family Proteins in Plants. Plant Physiol. 138: 1446-1456 [Abstract] [Full Text]  
  • Park, Y.-J., Chodaparambil, J. V., Bao, Y., McBryant, S. J., Luger, K. (2005). Nucleosome Assembly Protein 1 Exchanges Histone H2A-H2B Dimers and Assists Nucleosome Sliding. J. Biol. Chem. 280: 1817-1825 [Abstract] [Full Text]  
  • Opal, P., Garcia, J. J., McCall, A. E., Xu, B., Weeber, E. J., Sweatt, J. D., Orr, H. T., Zoghbi, H. Y. (2004). Generation and Characterization of LANP/pp32 Null Mice. Mol. Cell. Biol. 24: 3140-3149 [Abstract] [Full Text]  
  • Rehtanz, M., Schmidt, H.-M., Warthorst, U., Steger, G. (2004). Direct Interaction between Nucleosome Assembly Protein 1 and the Papillomavirus E2 Proteins Involved in Activation of Transcription. Mol. Cell. Biol. 24: 2153-2168 [Abstract] [Full Text]  
  • McBryant, S. J., Park, Y.-J., Abernathy, S. M., Laybourn, P. J., Nyborg, J. K., Luger, K. (2003). Preferential Binding of the Histone (H3-H4)2 Tetramer by NAP1 Is Mediated by the Amino-terminal Histone Tails. J. Biol. Chem. 278: 44574-44583 [Abstract] [Full Text]  
  • Miyaji-Yamaguchi, M., Kato, K., Nakano, R., Akashi, T., Kikuchi, A., Nagata, K. (2003). Involvement of Nucleocytoplasmic Shuttling of Yeast Nap1 in Mitotic Progression. Mol. Cell. Biol. 23: 6672-6684 [Abstract] [Full Text]  
  • Asahara, H., Tartare-Deckert, S., Nakagawa, T., Ikehara, T., Hirose, F., Hunter, T., Ito, T., Montminy, M. (2002). Dual Roles of p300 in Chromatin Assembly and Transcriptional Activation in Cooperation with Nucleosome Assembly Protein 1 In Vitro. Mol. Cell. Biol. 22: 2974-2983 [Abstract] [Full Text]  
  • Fan, Z., Beresford, P. J., Zhang, D., Lieberman, J. (2002). HMG2 Interacts with the Nucleosome Assembly Protein SET and Is a Target of the Cytotoxic T-Lymphocyte Protease Granzyme A. Mol. Cell. Biol. 22: 2810-2820 [Abstract] [Full Text]  
  • Ray, S., Sherman, C. T., Lu, M., Brasier, A. R. (2002). Angiotensinogen Gene Expression Is Dependent on Signal Transducer and Activator of Transcription 3-Mediated p300/cAMP Response Element Binding Protein-Binding Protein Coactivator Recruitment and Histone Acetyltransferase Activity. Mol. Endocrinol. 16: 824-836 [Abstract] [Full Text]  
  • Beresford, P. J., Zhang, D., Oh, D. Y., Fan, Z., Greer, E. L., Russo, M. L., Jaju, M., Lieberman, J. (2001). Granzyme A Activates an Endoplasmic Reticulum-associated Caspase-independent Nuclease to Induce Single-stranded DNA Nicks. J. Biol. Chem. 276: 43285-43293 [Abstract] [Full Text]  
  • Manning, E. T., Ikehara, T., Ito, T., Kadonaga, J. T., Kraus, W. L. (2001). p300 Forms a Stable, Template-Committed Complex with Chromatin: Role for the Bromodomain. Mol. Cell. Biol. 21: 3876-3887 [Abstract] [Full Text]  
  • Chan, H. M., La Thangue, N. B. (2001). p300/CBP proteins: HATs for transcriptional bridges and scaffolds. J. Cell Sci. 114: 2363-2373 [Abstract] [Full Text]  
  • Monroy, M. A., Ruhl, D. D., Xu, X., Granner, D. K., Yaciuk, P., Chrivia, J. C. (2001). Regulation of cAMP-responsive Element-binding Protein-mediated Transcription by the SNF2/SWI-related Protein, SRCAP. J. Biol. Chem. 276: 40721-40726 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»