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Molecular and Cellular Biology, February 2000, p. 755-759, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Normal Development, Wound Healing, and Adenovirus Susceptibility in beta 5-Deficient Mice

Xiaozhu Huang,1,2 Mark Griffiths,1,2,dagger Jianfeng Wu,1,2 Robert V. Farese Jr.,2,3 and Dean Sheppard1,2,*

Lung Biology Center,1 Gladstone Institute of Cardiovascular Disease,3 and Department of Medicine,2 University of California, San Francisco, San Francisco, California 94143-0854

Received 19 October 1999/Accepted 27 October 1999

Integrins have been shown to play important roles in embryonic development, wound healing, metastasis, and other biological processes. alpha vbeta 5 is a receptor for RGD-containing extracellular matrix proteins that has been suggested to be important in cutaneous wound healing and adenovirus infection. To examine the in vivo function of this receptor, we have generated mice lacking beta 5 expression, using homologous recombination in embryonic stem cells. Mice homozygous for a null mutation of the beta 5 subunit gene develop, grow, and reproduce normally. Keratinocytes harvested from beta 5-/- mice demonstrate impaired migration on and adhesion to the alpha vbeta 5 ligand, vitronectin. However, the rate of healing of cutaneous wounds is not different in beta 5-/- and beta 5+/+ mice. Furthermore, keratinocytes and airway epithelial cells obtained from null mice show adenovirus infection efficiency equal to that from wild-type mice. These data suggest that alpha vbeta 5 is not essential for normal development, reproduction, adenovirus infection, or the healing of cutaneous wounds.

* Corresponding author. Mailing address: Lung Biology Center, UCSF Box 0854, San Francisco, CA 94143-0854. Phone: (415) 206-5901. Fax: (415) 206-4123. E-mail: deans{at}itsa.ucsf.edu.

dagger Present address: 76 Bolingbroke Grove, London SW11 6HB, United Kingdom.

Molecular and Cellular Biology, February 2000, p. 755-759, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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