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Molecular and Cellular Biology, February 2000, p. 770-778, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

p53 Mutants Have Selective Dominant-Negative Effects on Apoptosis but Not Growth Arrest in Human Cancer Cell Lines

Oscar N. Aurelio,dagger Xiao-Tang Kong, Swati Gupta, and Eric J. Stanbridge*

Department of Microbiology and Molecular Genetics, University of California---Irvine, College of Medicine, Irvine, California 92697-4025

Received 22 July 1999/Returned for modification 9 September 1999/Accepted 26 October 1999

A bidirectional expression vector that allowed equal transcription of cloned wild-type and mutant p53 cDNAs from the same vector was developed. The vector was transfected into CaLu 6 lung carcinoma cells or Saos-2 osteosarcoma cells. All p53 mutants examined were recessive to wild-type p53 transactivation of p21WAF1/CIP1 but dominant-negative for transactivation of Bax. An examination of effects on growth arrest and apoptotic pathways indicated that all mutants were recessive to wild type for growth arrest but only three of seven mutants were dominant negative for induction of apoptosis.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, University of California---Irvine, College of Medicine, Irvine, CA 92697-4025. Phone: (949) 824-7042. Fax: (949) 824-8598. E-mail: ejstanbr{at}uci.edu.

dagger Present address: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.

Molecular and Cellular Biology, February 2000, p. 770-778, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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