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Molecular and Cellular Biology, February 2000, p. 919-928, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mutations in Host Cell Factor 1 Separate Its Role in Cell Proliferation from Recruitment of VP16 and LZIP

Shahana S. Mahajan and Angus C. Wilson*

Department of Microbiology and Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016

Received 8 September 1999/Returned for modification 13 October 1999/Accepted 1 November 1999

Host cell factor 1 (HCF-1) is a nuclear protein required for progression through G1 phase of the cell cycle and, via its association with VP16, transcriptional activation of the herpes simplex virus immediate-early genes. Both functions require a six-bladed beta -propeller domain encoded by residues 1 to 380 of HCF-1 as well as an additional amino-terminal region. The beta -propeller domain is well conserved in HCF homologues, consistent with a critical cellular function. To date, the only known cellular target of the beta -propeller is a bZIP transcription factor known as LZIP or Luman. Whether the interaction between HCF-1 and LZIP is required for cell proliferation remains to be determined. In this study, we used directed mutations to show that all six blades of the HCF-1 beta -propeller contribute to VP16-induced complex assembly, association with LZIP, and cell cycle progression. Although LZIP and VP16 share a common tetrapeptide HCF-binding motif, our results reveal profound differences in their interaction with HCF-1. Importantly, with several of the mutants we observe a poor correlation between the ability to associate with LZIP and promote cell proliferation in the context of the full HCF-1 amino terminus, arguing that the HCF-1 beta -propeller domain must target other cellular transcription factors in order to contribute to G1 progression.

* Corresponding author. Mailing address: Department of Microbiology, 550 First Ave., New York, NY 10016. Phone: (212) 263-0206. Fax: (212) 263-8276. E-mail: wilsoa02{at}popmail.med.nyu.edu.

Molecular and Cellular Biology, February 2000, p. 919-928, Vol. 20, No. 3
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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