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Molecular and Cellular Biology, February 2000, p. 1227-1233, Vol. 20, No. 4
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The B-Cell-Specific src-Family Kinase Blk Is Dispensable for B-Cell Development and Activation

Gemma Texido,1,dagger I-hsin Su,2 Ingrid Mecklenbräuker,2 Kaoru Saijo,2 Sami N. Malek,3 Stephen Desiderio,3 Klaus Rajewsky,1 and Alexander Tarakhovsky2,*

Department of Immunology1 and Laboratory of Lymphocyte Signaling,2 Institute for Genetics, University of Köln, D-50931 Cologne, Germany, and Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 212053

Received 5 August 1999/Returned for modification 15 September 1999/Accepted 12 November 1999

The B-cell lymphocyte kinase (Blk) is a src-family protein tyrosine kinase specifically expressed in B-lineage cells of mice. The early onset of Blk expression during B-cell development in the bone marrow and the high expression levels of Blk in mature B cells suggest a possible important role of Blk in B-cell physiology. To study the in vivo function of Blk, mice homozygous for the targeted disruption of the blk gene were generated. In homozygous mutant mice, neither blk mRNA nor Blk protein is expressed. Despite the absence of Blk, the development, in vitro activation, and humoral immune responses of B cells to T-cell-dependent and -independent antigens are unaltered. These data are consistent with functional redundancy of Blk in B-cell development and immune responses.

* Corresponding author. Mailing address: Laboratory of Lymphocyte Signaling, Institute for Genetics, University of Köln, Weyertal 121, D-50931 Cologne, Germany. Phone: 49-221-470 3419. Fax: 49-221-470 4970. E-mail: sasha{at}mac.genetik.uni-koeln.de.

dagger Present address: EMBL, D-69117 Heidelberg, Germany.

Molecular and Cellular Biology, February 2000, p. 1227-1233, Vol. 20, No. 4
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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