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Molecular and Cellular Biology, March 2000, p. 1616-1625, Vol. 20, No. 5
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cubitus interruptus Requires Drosophila CREB-Binding Protein To Activate wingless Expression in the Drosophila Embryo

Yang Chen,1,dagger R. H. Goodman,1 and Sarah M. Smolik2,*

Vollum Institute1 and Department of Cell and Developmental Biology,2 Oregon Health Sciences University, Portland, Oregon 97201

Received 29 July 1999/Returned for modification 23 September 1999/Accepted 23 November 1999

CREB-binding protein (CBP) serves as a transcriptional coactivator in multiple signal transduction pathways. The Drosophila homologue of CBP, dCBP, interacts with the transcription factors Cubitus interruptus (CI), MAD, and Dorsal (DL) and functions as a coactivator in several signaling pathways during Drosophila development, including the hedgehog (hh), decapentaplegic (dpp), and Toll pathways. Although dCBP is required for the expression of the hh target genes, wingless (wg) and patched (ptc) in vivo, and potentiates ci-mediated transcriptional activation in vitro, it is not known that ci absolutely requires dCBP for its activity. We used a yeast genetic screen to identify several ci point mutations that disrupt CI-dCBP interactions. These mutant proteins are unable to transactivate a reporter gene regulated by ci binding sites and have a lower dCBP-stimulated activity than wild-type CI. When expressed exogenously in embryos, the CI point mutants cannot activate endogenous wg expression. Furthermore, a CI mutant protein that lacks the entire dCBP interaction domain functions as a negative competitor for wild-type CI activity, and the expression of dCBP antisense RNAs can suppress CI transactivation in Kc cells. Taken together, our data suggest that dCBP function is necessary for ci-mediated transactivation of wg during Drosophila embryogenesis.

* Corresponding author. Mailing address: Department of Cell and Developmental Biology, L-215, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd., Portland, OR 97201. Phone: (503) 494-7192. Fax: (503) 494-4353. E-mail: smoliks{at}ohsu.edu.

dagger Present address: Myriad Genetics, Inc., Salt Lake City, UT 84108.

Molecular and Cellular Biology, March 2000, p. 1616-1625, Vol. 20, No. 5
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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