This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bera, T. K.
Right arrow Articles by Pastan, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bera, T. K.
Right arrow Articles by Pastan, I.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2000, p. 2902-2906, Vol. 20, No. 8
0270-7306/00/$04.00+0

Mesothelin Is Not Required for Normal Mouse Development or Reproduction

Tapan K. Bera and Ira Pastan*

Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255

Received 6 December 1999/Returned for modification 8 December 1999/Accepted 28 December 1999

Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function(s) of the protein is not known. We have analyzed the expression of the mouse mesothelin gene in different developmental stages and in various adult tissues by Northern hybridization. The 2.5-kb mesothelin transcript was detected in the mRNA of E 7.0, E 15.0, and E 17.0 stages of mouse development. In adult tissues the mesothelin gene was expressed in lung, heart, spleen, liver, kidney, and testis. To directly assess the function of the mesothelin in vivo, we generated mutant mice in which the mesothelin gene was inactivated by replacing it with the neomycin resistance gene. In homozygous mutant mice neither mesothelin mRNA nor the protein product was detected. Null mutant mice were obtained in accordance with Mendelian laws, and both males and females produced offspring normally. No anatomical or histological abnormalities were detected in any tissues where mesothelin was reportedly expressed in wild-type mice. Our results demonstrate that mesothelin function is not essential for growth or reproduction in mice.

* Corresponding author. Mailing address: Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Building 37, Room 4E16, 37 Convent Dr., MSC 4255, Bethesda, MD. Phone: (301) 496-4797. Fax: (301) 402-1344. E-mail: pasta{at}helix.nih.gov.

Molecular and Cellular Biology, April 2000, p. 2902-2906, Vol. 20, No. 8
0270-7306/00/$04.00+0


This article has been cited by other articles:

  • Johnston, F. Mc., Tan, M. C.B., Tan, B. R. Jr., Porembka, M. R., Brunt, E. M., Linehan, D. C., Simon, P. O. Jr., Plambeck-Suess, S., Eberlein, T. J., Hellstrom, K. E., Hellstrom, I., Hawkins, W. G., Goedegebuure, P. (2009). Circulating Mesothelin Protein and Cellular Antimesothelin Immunity in Patients with Pancreatic Cancer. Clin. Cancer Res. 15: 6511-6518 [Abstract] [Full Text]  
  • Feng, Y., Xiao, X., Zhu, Z., Streaker, E., Ho, M., Pastan, I., Dimitrov, D. S. (2009). A novel human monoclonal antibody that binds with high affinity to mesothelin-expressing cells and kills them by antibody-dependent cell-mediated cytotoxicity. Molecular Cancer Therapeutics 8: 1113-1118 [Abstract] [Full Text]  
  • Johnson, M. D., Vito, F., O'Connell, M. J. (2008). Mesothelin Expression in the Leptomeninges and Meningiomas. J. Histochem. Cytochem. 56: 579-585 [Abstract] [Full Text]  
  • Uehara, N., Matsuoka, Y., Tsubura, A. (2008). Mesothelin Promotes Anchorage-Independent Growth and Prevents Anoikis via Extracellular Signal-Regulated Kinase Signaling Pathway in Human Breast Cancer Cells. Mol Cancer Res 6: 186-193 [Abstract] [Full Text]  
  • Hucl, T., Brody, J. R., Gallmeier, E., Iacobuzio-Donahue, C. A., Farrance, I. K., Kern, S. E. (2007). High Cancer-Specific Expression of Mesothelin (MSLN) Is Attributable to an Upstream Enhancer Containing a Transcription Enhancer Factor Dependent MCAT Motif. Cancer Res. 67: 9055-9065 [Abstract] [Full Text]  
  • Robinson, C., van Bruggen, I., Segal, A., Dunham, M., Sherwood, A., Koentgen, F., Robinson, B. W.S., Lake, R. A. (2006). A Novel SV40 TAg Transgenic Model of Asbestos-Induced Mesothelioma: Malignant Transformation Is Dose Dependent.. Cancer Res. 66: 10786-10794 [Abstract] [Full Text]  
  • Onda, M., Nagata, S., Ho, M., Bera, T. K., Hassan, R., Alexander, R. H., Pastan, I. (2006). Megakaryocyte potentiation factor cleaved from mesothelin precursor is a useful tumor marker in the serum of patients with mesothelioma.. Clin. Cancer Res. 12: 4225-4231 [Abstract] [Full Text]  
  • Yen, M. J., Hsu, C.-Y., Mao, T.-L., Wu, T-C., Roden, R., Wang, T.-L., Shih, I.-M. (2006). Diffuse Mesothelin Expression Correlates with Prolonged Patient Survival in Ovarian Serous Carcinoma. Clin. Cancer Res. 12: 827-831 [Abstract] [Full Text]  
  • Hassan, R., Remaley, A. T., Sampson, M. L., Zhang, J., Cox, D. D., Pingpank, J., Alexander, R., Willingham, M., Pastan, I., Onda, M. (2006). Detection and Quantitation of Serum Mesothelin, a Tumor Marker for Patients with Mesothelioma and Ovarian Cancer. Clin. Cancer Res. 12: 447-453 [Abstract] [Full Text]  
  • Onda, M., Willingham, M., Nagata, S., Bera, T. K., Beers, R., Ho, M., Hassan, R., Kreitman, R. J., Pastan, I. (2005). New Monoclonal Antibodies to Mesothelin Useful for Immunohistochemistry, Fluorescence-Activated Cell Sorting, Western Blotting, and ELISA. Clin. Cancer Res. 11: 5840-5846 [Abstract] [Full Text]  
  • Ho, M., Hassan, R., Zhang, J., Wang, Q.-c., Onda, M., Bera, T., Pastan, I. (2005). Humoral Immune Response to Mesothelin in Mesothelioma and Ovarian Cancer Patients. Clin. Cancer Res. 11: 3814-3820 [Abstract] [Full Text]  
  • Rump, A., Morikawa, Y., Tanaka, M., Minami, S., Umesaki, N., Takeuchi, M., Miyajima, A. (2004). Binding of Ovarian Cancer Antigen CA125/MUC16 to Mesothelin Mediates Cell Adhesion. J. Biol. Chem. 279: 9190-9198 [Abstract] [Full Text]  
  • Hassan, R., Lerner, M. R., Benbrook, D., Lightfoot, S. A., Brackett, D. J., Wang, Q.-C., Pastan, I. (2002). Antitumor Activity of SS(dsFv)PE38 and SS1(dsFv)PE38, Recombinant Antimesothelin Immunotoxins against Human Gynecologic Cancers Grown in Organotypic Culture in Vitro. Clin. Cancer Res. 8: 3520-3526 [Abstract] [Full Text]  
  • Fan, D., Yano, S., Shinohara, H., Solorzano, C., Van Arsdall, M., Bucana, C. D., Pathak, S., Kruzel, E., Herbst, R. S., Onn, A., Roach, J. S., Onda, M., Wang, Q.-c., Pastan, I., Fidler, I. J. (2002). Targeted Therapy against Human Lung Cancer in Nude Mice by High-Affinity Recombinant Antimesothelin Single-Chain Fv Immunotoxin. Molecular Cancer Therapeutics 1: 595-600 [Abstract] [Full Text]  
  • Bera, T. K., Bera, J., Brinkmann, U., Tessarollo, L., Pastan, I. (2001). Cse1l Is Essential for Early Embryonic Growth and Development. Mol. Cell. Biol. 21: 7020-7024 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»