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Molecular and Cellular Biology, July 2001, p. 4089-4096, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4089-4096.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Phosphorylation of the RNA Polymerase II Carboxy-Terminal Domain by the Bur1 Cyclin-Dependent Kinase

Stuart Murray,1,dagger Rajesh Udupa,1 Sheng Yao,1 Grant Hartzog,2 and Gregory Prelich1,*

Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461,1 and Department of Biology, Sinsheimer Labs, University of California at Santa Cruz, Santa Cruz, California 950642

Received 17 January 2001/Returned for modification 13 February 2001/Accepted 9 April 2001

BUR1, which was previously identified by a selection for mutations that have general effects on transcription in Saccharomyces cerevisiae, encodes a cyclin-dependent kinase that is essential for viability, but none of its substrates have been identified to date. Using an unbiased biochemical approach, we have identified the carboxy-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II, as a Bur1 substrate. Phosphorylation of Rpb1 by Bur1 is likely to be physiologically relevant, since bur1 mutations interact genetically with rpb1 CTD truncations and with mutations in other genes involved in CTD function. Several genetic interactions are presented, implying a role for Bur1 during transcriptional elongation. These results identify Bur1 as a fourth S. cerevisiae CTD kinase and provide striking functional similarities between Bur1 and metazoan P-TEFb.

* Corresponding author. Mailing address: Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-2181. Fax: (718) 430-8778. E-mail: prelich{at}aecom.yu.edu.

dagger Present address: Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.

Molecular and Cellular Biology, July 2001, p. 4089-4096, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4089-4096.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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