This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Roermund, C. W. T.
Right arrow Articles by Wanders, R. J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Roermund, C. W. T.
Right arrow Articles by Wanders, R. J. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2001, p. 4321-4329, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4321-4329.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of a Peroxisomal ATP Carrier Required for Medium-Chain Fatty Acid beta -Oxidation and Normal Peroxisome Proliferation in Saccharomyces cerevisiae

Carlo W. T. van Roermund,1 Roy Drissen,1 Marlene van den Berg,2 Lodewijk Ijlst,1 Ewald H. Hettema,2 Henk F. Tabak,2 Hans R. Waterham,1,3 and Ronald J. A. Wanders1,3,*

University of Amsterdam, Academic Medical Centre, Departments of Clinical Chemistry,1 Biochemistry,2 and Paediatrics,3 Emma Children's Hospital, 1100 DE Amsterdam, The Netherlands

Received 18 December 2000/Returned for modification 6 February 2001/Accepted 4 April 2001

We have characterized the role of YPR128cp, the orthologue of human PMP34, in fatty acid metabolism and peroxisomal proliferation in Saccharomyces cerevisiae. YPR128cp belongs to the mitochondrial carrier family (MCF) of solute transporters and is localized in the peroxisomal membrane. Disruption of the YPR128c gene results in impaired growth of the yeast with the medium-chain fatty acid (MCFA) laurate as a single carbon source, whereas normal growth was observed with the long-chain fatty acid (LCFA) oleate. MCFA but not LCFA beta -oxidation activity was markedly reduced in intact ypr128cDelta mutant cells compared to intact wild-type cells, but comparable activities were found in the corresponding lysates. These results imply that a transport step specific for MCFA beta -oxidation is impaired in ypr128cDelta cells. Since MCFA beta -oxidation in peroxisomes requires both ATP and CoASH for activation of the MCFAs into their corresponding coenzyme A esters, we studied whether YPR128cp is an ATP carrier. For this purpose we have used firefly luciferase targeted to peroxisomes to measure ATP consumption inside peroxisomes. We show that peroxisomal luciferase activity was strongly reduced in intact ypr128cDelta mutant cells compared to wild-type cells but comparable in lysates of both cell strains. We conclude that YPR128cp most likely mediates the transport of ATP across the peroxisomal membrane.

* Corresponding author. Mailing address: University of Amsterdam, Academic Medical Centre, Meibergdreef 9 (Room F0-224), 1105 AZ Amsterdam, The Netherlands. Phone: 31-20-5662427. Fax: 31-20-6962596. E-mail: wanders{at}amc.uva.nl.

Molecular and Cellular Biology, July 2001, p. 4321-4329, Vol. 21, No. 13
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.13.4321-4329.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • van Roermund, C. W. T., Visser, W. F., IJlst, L., van Cruchten, A., Boek, M., Kulik, W., Waterham, H. R., Wanders, R. J. A. (2008). The human peroxisomal ABC half transporter ALDP functions as a homodimer and accepts acyl-CoA esters. FASEB J. 22: 4201-4208 [Abstract] [Full Text]  
  • Linka, N., Theodoulou, F. L., Haslam, R. P., Linka, M., Napier, J. A., Neuhaus, H. E., Weber, A. P.M. (2008). Peroxisomal ATP Import Is Essential for Seedling Development in Arabidopsis thaliana. Plant Cell 20: 3241-3257 [Abstract] [Full Text]  
  • Strijbis, K., van Roermund, C. W. T., Visser, W. F., Mol, E. C., van den Burg, J., MacCallum, D. M., Odds, F. C., Paramonova, E., Krom, B. P., Distel, B. (2008). Carnitine-Dependent Transport of Acetyl Coenzyme A in Candida albicans Is Essential for Growth on Nonfermentable Carbon Sources and Contributes to Biofilm Formation. Eukaryot Cell 7: 610-618 [Abstract] [Full Text]  
  • Hynes, M. J., Murray, S. L., Khew, G. S., Davis, M. A. (2008). Genetic Analysis of the Role of Peroxisomes in the Utilization of Acetate and Fatty Acids in Aspergillus nidulans. Genetics 178: 1355-1369 [Abstract] [Full Text]  
  • Cavalier-Smith, T. (2006). Origin of mitochondria by intracellular enslavement of a photosynthetic purple bacterium. Proc R Soc B 273: 1943-1952 [Abstract] [Full Text]  
  • Colasante, C., Alibu, V. P., Kirchberger, S., Tjaden, J., Clayton, C., Voncken, F. (2006). Characterization and Developmentally Regulated Localization of the Mitochondrial Carrier Protein Homologue MCP6 from Trypanosoma brucei.. Eukaryot Cell 5: 1194-1205 [Abstract] [Full Text]  
  • Zhang, B., Carlson, R., Srienc, F. (2006). Engineering the Monomer Composition of Polyhydroxyalkanoates Synthesized in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 72: 536-543 [Abstract] [Full Text]  
  • Antonenkov, V. D., Sormunen, R. T., Hiltunen, J. K. (2004). The rat liver peroxisomal membrane forms a permeability barrier for cofactors but not for small metabolites in vitro. J. Cell Sci. 117: 5633-5642 [Abstract] [Full Text]  
  • van Roermund, C. W. T., de Jong, M., IJlst, L., van Marle, J., Dansen, T. B., Wanders, R. J. A., Waterham, H. R. (2004). The peroxisomal lumen in Saccharomyces cerevisiae is alkaline. J. Cell Sci. 117: 4231-4237 [Abstract] [Full Text]  
  • del Arco, A., Satrustegui, J. (2004). Identification of a Novel Human Subfamily of Mitochondrial Carriers with Calcium-binding Domains. J. Biol. Chem. 279: 24701-24713 [Abstract] [Full Text]  
  • Voncken, F., van Hellemond, J. J., Pfisterer, I., Maier, A., Hillmer, S., Clayton, C. (2003). Depletion of GIM5 Causes Cellular Fragility, a Decreased Glycosome Number, and Reduced Levels of Ether-linked Phospholipids in Trypanosomes. J. Biol. Chem. 278: 35299-35310 [Abstract] [Full Text]  
  • Li, X., Gould, S. J. (2002). PEX11 promotes peroxisome division independently of peroxisome metabolism. JCB 156: 643-651 [Abstract] [Full Text]  
  • Li, X., Gould, S. J. (2002). PEX11 promotes peroxisome division independently of peroxisome metabolism. JCB 156: 643-651 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»