This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yeh, J.-H.
Right arrow Articles by Imbert, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yeh, J.-H.
Right arrow Articles by Imbert, J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2001, p. 4515-4527, Vol. 21, No. 14
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.14.4515-4527.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel CD28-Responsive Enhancer Activated by CREB/ATF and AP-1 Families in the Human Interleukin-2 Receptor alpha -Chain Locus

Jung-Hua Yeh,1 Patrick Lecine,1 Jacques A. Nunes,1 Salvatore Spicuglia,2 Pierre Ferrier,2 Daniel Olive,1 and Jean Imbert1,*

INSERM U119-IFR57, 13009 Marseilles,1 and Centre d'Immunologie CNRS-INSERM-Univ.Med., 13288 Marseille Cedex 09,2 France

Received 20 December 2000/Returned for modification 13 February 2001/Accepted 16 April 2001

The interaction of interleukin-2 (IL-2) with its receptor (IL-2R) critically regulates the T-cell immune response, and the alpha  chain CD25/IL-2Ralpha is required for the formation of the high-affinity receptor. Tissue-specific, inducible expression of the IL-2Ralpha gene is regulated by at least three positive regulatory regions (PRRI, PRRII, and PRRIII), but none responded to CD28 engagement in gene reporter assays although CD28 costimulation strongly amplifies IL-2Ralpha gene transcription. By DNase I hypersensitivity analysis, we have identified a novel TCR-CD3- and CD28-responsive enhancer (CD28rE) located 8.5 kb 5' of the IL-2Ralpha gene. PRRIV/CD28rE contains a functional CRE/TRE element required for CD28 signaling. The T-cell-specific, CD28-responsive expression of the IL-2Ralpha gene appears controlled through PRRIV/CD28rE by cooperation of CREB/ATF and AP-1 family transcription factors.

* Corresponding author. Mailing address: INSERM U119-IFR57, 27 Blvd. Lei Roure, 13009 Marseilles, France. Phone: 33 (0)491 75 84 04. Fax: 33 (0)491 26 03 64. E-mail: imbert{at}marseille.inserm.fr.

Molecular and Cellular Biology, July 2001, p. 4515-4527, Vol. 21, No. 14
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.14.4515-4527.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hua, G., Zhu, B., Rosa, F., Deblon, N., Adelaide, J., Kahn-Perles, B., Birnbaum, D., Imbert, J. (2009). A Negative Feedback Regulatory Loop Associates the Tyrosine Kinase Receptor ERBB2 and the Transcription Factor GATA4 in Breast Cancer Cells. Mol Cancer Res 7: 402-414 [Abstract] [Full Text]  
  • Samten, B., Townsend, J. C., Weis, S. E., Bhoumik, A., Klucar, P., Shams, H., Barnes, P. F. (2008). CREB, ATF, and AP-1 Transcription Factors Regulate IFN-{gamma} Secretion by Human T Cells in Response to Mycobacterial Antigen. J. Immunol. 181: 2056-2064 [Abstract] [Full Text]  
  • Schneider, O. D., Weiss, A. A., Miller, W. E. (2007). Pertussis Toxin Utilizes Proximal Components of the T-Cell Receptor Complex To Initiate Signal Transduction Events in T Cells. Infect. Immun. 75: 4040-4049 [Abstract] [Full Text]  
  • Zhou, L., Nazarian, A. A., Xu, J., Tantin, D., Corcoran, L. M., Smale, S. T. (2007). An Inducible Enhancer Required for Il12b Promoter Activity in an Insulated Chromatin Environment. Mol. Cell. Biol. 27: 2698-2712 [Abstract] [Full Text]  
  • Kim, H.-P., Kim, B.-G., Letterio, J., Leonard, W. J. (2005). Smad-dependent Cooperative Regulation of Interleukin 2 Receptor {alpha} Chain Gene Expression by T Cell Receptor and Transforming Growth Factor-{beta}. J. Biol. Chem. 280: 34042-34047 [Abstract] [Full Text]  
  • O'Sullivan, A., Chang, H.-C., Yu, Q., Kaplan, M. H. (2004). STAT4 Is Required for Interleukin-12-induced Chromatin Remodeling of the CD25 Locus. J. Biol. Chem. 279: 7339-7345 [Abstract] [Full Text]  
  • Mitchell, T. J., Whittaker, S. J., John, S. (2003). Dysregulated Expression of COOH-Terminally Truncated Stat5 and Loss of IL2-Inducible Stat5-Dependent Gene Expression in Sezary Syndrome. Cancer Res. 63: 9048-9054 [Abstract] [Full Text]  
  • Kovanen, P. E., Rosenwald, A., Fu, J., Hurt, E. M., Lam, L. T., Giltnane, J. M., Wright, G., Staudt, L. M., Leonard, W. J. (2003). Analysis of gamma c-Family Cytokine Target Genes. IDENTIFICATION OF DUAL-SPECIFICITY PHOSPHATASE 5 (DUSP5) AS A REGULATOR OF MITOGEN-ACTIVATED PROTEIN KINASE ACTIVITY IN INTERLEUKIN-2 SIGNALING. J. Biol. Chem. 278: 5205-5213 [Abstract] [Full Text]  
  • Wong, A. W., Ghosh, N., McKinnon, K. P., Reed, W., Piskurich, J. F., Wright, K. L., Ting, J. P.-Y. (2002). Regulation and Specificity of MHC2TA Promoter Usage in Human Primary T Lymphocytes and Cell Line. J. Immunol. 169: 3112-3119 [Abstract] [Full Text]  
  • Yeh, J.-H., Spicuglia, S., Kumar, S., Sanchez-Sevilla, A., Ferrier, P., Imbert, J. (2002). Control of IL-2R{alpha} gene expression: structural changes within the proximal enhancer/core promoter during T-cell development. Nucleic Acids Res 30: 1944-1951 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»