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Molecular and Cellular Biology, October 2001, p. 6681-6694, Vol. 21, No. 19
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.19.6681-6694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Study of Cyclin Proteolysis in Anaphase-Promoting Complex (APC) Mutant Cells Reveals the Requirement for APC Function in the Final Steps of the Fission Yeast Septation Initiation Network

Louise Chang,dagger Jennifer L. Morrell, Anna Feoktistova, and Kathleen L. Gould*

Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Received 15 May 2001/Returned for modification 26 June 2001/Accepted 5 July 2001

Cytokinesis in eukaryotic cells requires the inactivation of mitotic cyclin-dependent kinase complexes. An apparent exception to this relationship is found in Schizosaccharomyces pombe mutants with mutations of the anaphase-promoting complex (APC). These conditional lethal mutants arrest with unsegregated chromosomes because they cannot degrade the securin, Cut2p. Although failing at nuclear division, these mutants septate and divide. Since septation requires Cdc2p inactivation in wild-type S. pombe, it has been suggested that Cdc2p inactivation occurs in these mutants by a mechanism independent of cyclin degradation. In contrast to this prediction, we show that Cdc2p kinase activity fluctuates in APC cut mutants due to Cdc13/cyclin B destruction. In APC-null mutants, however, septation and cutting do not occur and Cdc13p is stable. We conclude that APC cut mutants are hypomorphic with respect to Cdc13p degradation. Indeed, overproduction of nondestructible Cdc13p prevents septation in APC cut mutants and the normal reorganization of septation initiation network components during anaphase.

* Corresponding author. Mailing address: B2309 MCN, 1161 21st Ave. S, Nashville, TN 37232. Phone: (615) 343-9502. Fax: (615) 343-0723. E-mail: Kathy.Gould{at}mcmail.vanderbilt.edu.

dagger Present address: Division of Medical Genetics, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109-0650.

Molecular and Cellular Biology, October 2001, p. 6681-6694, Vol. 21, No. 19
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.19.6681-6694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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