This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shen, Y.
Right arrow Articles by Foster, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shen, Y.
Right arrow Articles by Foster, D. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2001, p. 595-602, Vol. 21, No. 2
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.2.595-602.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role for Phospholipase D in Receptor-Mediated Endocytosis

Yingjie Shen, Lizhong Xu, and David A. Foster*

Department of Biological Sciences, Hunter College of The City University of New York, New York, New York 10021

Received 22 June 2000/Returned for modification 27 July 2000/Accepted 12 October 2000

In response to epidermal growth factor (EGF), the EGF receptor is endocytosed and degraded. A substantial lag period exists between endocytosis and degradation, suggesting that endocytosis is more than a simple negative feedback. Phospholipase D (PLD), which has been implicated in vesicle formation in the Golgi, is activated in response to EGF and other growth factors. We report here that EGF receptor endocytosis is dependent upon PLD and the PLD1 regulators, protein kinase C alpha  and RalA. EGF-induced receptor degradation is accelerated by overexpression of either wild-type PLD1 or PLD2 and retarded by overexpression of catalytically inactive mutants of either PLD1 or PLD2. EGF-induced activation of mitogen-activated protein kinase, which is dependent upon receptor endocytosis, is also dependent upon PLD. These data suggest a role for PLD in signaling that facilitates receptor endocytosis.

* Corresponding author. Mailing address: Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Ave., New York, NY 10021. Phone: (212) 772-4075. Fax: (212) 772-5227. E-mail: foster{at}genectr.hunter.cuny.edu.

Molecular and Cellular Biology, January 2001, p. 595-602, Vol. 21, No. 2
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.2.595-602.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Garrido, J. L., Wheeler, D., Vega, L. L., Friedman, P. A., Romero, G. (2009). Role of Phospholipase D in Parathyroid Hormone Type 1 Receptor Signaling and Trafficking. Mol. Endocrinol. 23: 2048-2059 [Abstract] [Full Text]  
  • Mor, A., Wynne, J. P., Ahearn, I. M., Dustin, M. L., Du, G., Philips, M. R. (2009). Phospholipase D1 Regulates Lymphocyte Adhesion via Upregulation of Rap1 at the Plasma Membrane. Mol. Cell. Biol. 29: 3297-3306 [Abstract] [Full Text]  
  • Guo, H.-B., Johnson, H., Randolph, M., Lee, I., Pierce, M. (2009). Knockdown of GnT-Va expression inhibits ligand-induced downregulation of the epidermal growth factor receptor and intracellular signaling by inhibiting receptor endocytosis. Glycobiology 19: 547-559 [Abstract] [Full Text]  
  • Toschi, A., Lee, E., Xu, L., Garcia, A., Gadir, N., Foster, D. A. (2009). Regulation of mTORC1 and mTORC2 Complex Assembly by Phosphatidic Acid: Competition with Rapamycin. Mol. Cell. Biol. 29: 1411-1420 [Abstract] [Full Text]  
  • Garcia, A., Zheng, Y., Zhao, C., Toschi, A., Fan, J., Shraibman, N., Brown, H. A., Bar-Sagi, D., Foster, D. A., Arbiser, J. L. (2008). Honokiol Suppresses Survival Signals Mediated by Ras-Dependent Phospholipase D Activity in Human Cancer Cells. Clin. Cancer Res. 14: 4267-4274 [Abstract] [Full Text]  
  • Lopez, J. A., Kwan, E. P., Xie, L., He, Y., James, D. E., Gaisano, H. Y. (2008). The RalA GTPase Is a Central Regulator of Insulin Exocytosis from Pancreatic Islet Beta Cells. J. Biol. Chem. 283: 17939-17945 [Abstract] [Full Text]  
  • Alvarez-Breckenridge, C. A., Waite, K. A., Eng, C. (2007). PTEN regulates phospholipase D and phospholipase C. Hum Mol Genet 16: 1157-1163 [Abstract] [Full Text]  
  • Grodnitzky, J. A., Syed, N., Kimber, M. J., Day, T. A., Donaldson, J. G., Hsu, W. H. (2007). Somatostatin Receptors Signal through EFA6A-ARF6 to Activate Phospholipase D in Clonal beta-Cells. J. Biol. Chem. 282: 13410-13418 [Abstract] [Full Text]  
  • Shmuel, M., Nodel-Berner, E., Hyman, T., Rouvinski, A., Altschuler, Y. (2007). Caveolin 2 Regulates Endocytosis and Trafficking of the M1 Muscarinic Receptor in MDCK Epithelial Cells. Mol. Biol. Cell 18: 1570-1585 [Abstract] [Full Text]  
  • Zheng, Y., Rodrik, V., Toschi, A., Shi, M., Hui, L., Shen, Y., Foster, D. A. (2006). Phospholipase D Couples Survival and Migration Signals in Stress Response of Human Cancer Cells. J. Biol. Chem. 281: 15862-15868 [Abstract] [Full Text]  
  • Kim, J. H., Kim, H.-w., Jeon, H., Suh, P.-G., Ryu, S. H. (2006). Phospholipase D1 Regulates Cell Migration in a Lipase Activity-independent Manner. J. Biol. Chem. 281: 15747-15756 [Abstract] [Full Text]  
  • Hornberger, T. A., Chu, W. K., Mak, Y. W., Hsiung, J. W., Huang, S. A., Chien, S. (2006). The role of phospholipase D and phosphatidic acid in the mechanical activation of mTOR signaling in skeletal muscle. Proc. Natl. Acad. Sci. USA 103: 4741-4746 [Abstract] [Full Text]  
  • Cai, D., Zhong, M., Wang, R., Netzer, W. J., Shields, D., Zheng, H., Sisodia, S. S., Foster, D. A., Gorelick, F. S., Xu, H., Greengard, P. (2006). Phospholipase D1 corrects impaired betaAPP trafficking and neurite outgrowth in familial Alzheimer's disease-linked presenilin-1 mutant neurons. Proc. Natl. Acad. Sci. USA 103: 1936-1940 [Abstract] [Full Text]  
  • Padron, D., Tall, R. D., Roth, M. G. (2006). Phospholipase D2 Is Required for Efficient Endocytic Recycling of Transferrin Receptors. Mol. Biol. Cell 17: 598-606 [Abstract] [Full Text]  
  • Kim, J., Lee, Y. H., Kwon, T. K., Chang, J.-S., Chung, K. C., Min, D. S. (2006). Phospholipase D Prevents Etoposide-Induced Apoptosis by Inhibiting the Expression of Early Growth Response-1 and Phosphatase and Tensin Homologue Deleted on Chromosome 10. Cancer Res. 66: 784-793 [Abstract] [Full Text]  
  • Lalli, G., Hall, A. (2005). Ral GTPases regulate neurite branching through GAP-43 and the exocyst complex. JCB 171: 857-869 [Abstract] [Full Text]  
  • Hui, L., Rodrik, V., Pielak, R. M., Knirr, S., Zheng, Y., Foster, D. A. (2005). mTOR-dependent Suppression of Protein Phosphatase 2A Is Critical for Phospholipase D Survival Signals in Human Breast Cancer Cells. J. Biol. Chem. 280: 35829-35835 [Abstract] [Full Text]  
  • Rodrik, V., Zheng, Y., Harrow, F., Chen, Y., Foster, D. A. (2005). Survival Signals Generated by Estrogen and Phospholipase D in MCF-7 Breast Cancer Cells Are Dependent on Myc. Mol. Cell. Biol. 25: 7917-7925 [Abstract] [Full Text]  
  • Vitale, N., Mawet, J., Camonis, J., Regazzi, R., Bader, M.-F., Chasserot-Golaz, S. (2005). The Small GTPase RalA Controls Exocytosis of Large Dense Core Secretory Granules by Interacting with ARF6-dependent Phospholipase D1. J. Biol. Chem. 280: 29921-29928 [Abstract] [Full Text]  
  • Huang, P., Altshuller, Y. M., Hou, J. C., Pessin, J. E., Frohman, M. A. (2005). Insulin-stimulated Plasma Membrane Fusion of Glut4 Glucose Transporter-containing Vesicles Is Regulated by Phospholipase D1. Mol. Biol. Cell 16: 2614-2623 [Abstract] [Full Text]  
  • Chen, Y., Rodrick, V., Yan, Y., Brazill, D. (2005). PldB, a Putative Phospholipase D Homologue in Dictyostelium discoideum Mediates Quorum Sensing during Development. Eukaryot Cell 4: 694-702 [Abstract] [Full Text]  
  • Hitomi, T., Zhang, J., Nicoletti, L. M., Grodzki, A. C. G., Jamur, M. C., Oliver, C., Siraganian, R. P. (2004). Phospholipase D1 regulates high-affinity IgE receptor-induced mast cell degranulation. Blood 104: 4122-4128 [Abstract] [Full Text]  
  • Bhattacharya, M., Babwah, A. V., Godin, C., Anborgh, P. H., Dale, L. B., Poulter, M. O., Ferguson, S. S. G. (2004). Ral and Phospholipase D2-Dependent Pathway for Constitutive Metabotropic Glutamate Receptor Endocytosis. J. Neurosci. 24: 8752-8761 [Abstract] [Full Text]  
  • Yamamoto, K., Takahashi, Y., Mano, T., Sakata, Y., Nishikawa, N., Yoshida, J., Oishi, Y., Hori, M., Miwa, T., Inoue, S., Masuyama, T. (2004). N-Methylethanolamine attenuates cardiac fibrosis and improves diastolic function: inhibition of phospholipase D as a possible mechanism. Eur Heart J 25: 1221-1229 [Abstract] [Full Text]  
  • Hui, L., Abbas, T., Pielak, R. M., Joseph, T., Bargonetti, J., Foster, D. A. (2004). Phospholipase D Elevates the Level of MDM2 and Suppresses DNA Damage-Induced Increases in p53. Mol. Cell. Biol. 24: 5677-5686 [Abstract] [Full Text]  
  • Hughes, W. E., Elgundi, Z., Huang, P., Frohman, M. A., Biden, T. J. (2004). Phospholipase D1 Regulates Secretagogue-stimulated Insulin Release in Pancreatic {beta}-Cells. J. Biol. Chem. 279: 27534-27541 [Abstract] [Full Text]  
  • Du, G., Huang, P., Liang, B. T., Frohman, M. A. (2004). Phospholipase D2 Localizes to the Plasma Membrane and Regulates Angiotensin II Receptor Endocytosis. Mol. Biol. Cell 15: 1024-1030 [Abstract] [Full Text]  
  • Wang, Y., Pennock, S. D., Chen, X., Kazlauskas, A., Wang, Z. (2004). Platelet-derived Growth Factor Receptor-mediated Signal Transduction from Endosomes. J. Biol. Chem. 279: 8038-8046 [Abstract] [Full Text]  
  • Larsen, J. E., Massol, R. H., Nieland, T. J. F., Kirchhausen, T. (2004). HIV Nef-mediated Major Histocompatibility Complex Class I Down-Modulation Is Independent of Arf6 Activity. Mol. Biol. Cell 15: 323-331 [Abstract] [Full Text]  
  • Lim, H.-K., Choi, Y.-A., Park, W., Lee, T., Ryu, S. H., Kim, S.-Y., Kim, J.-R., Kim, J.-H., Baek, S.-H. (2003). Phosphatidic Acid Regulates Systemic Inflammatory Responses by Modulating the Akt-Mammalian Target of Rapamycin-p70 S6 Kinase 1 Pathway. J. Biol. Chem. 278: 45117-45127 [Abstract] [Full Text]  
  • Wang, L., Cummings, R., Zhao, Y., Kazlauskas, A., Sham, J. K. S., Morris, A., Georas, S., Brindley, D. N., Natarajan, V. (2003). Involvement of Phospholipase D2 in Lysophosphatidate-induced Transactivation of Platelet-derived Growth Factor Receptor-{beta} in Human Bronchial Epithelial Cells. J. Biol. Chem. 278: 39931-39940 [Abstract] [Full Text]  
  • Coon, M., Ball, A., Pound, J., Ap, S., Hollenback, D., White, T., Tulinsky, J., Bonham, L., Morrison, D. K., Finney, R., Singer, J. W. (2003). Inhibition of lysophosphatidic acid acyltransferase {beta} disrupts proliferative and survival signals in normal cells and induces apoptosis of tumor cells. Molecular Cancer Therapeutics 2: 1067-1078 [Abstract] [Full Text]  
  • Mitchell, R., Robertson, D. N., Holland, P. J., Collins, D., Lutz, E. M., Johnson, M. S. (2003). ADP-ribosylation Factor-dependent Phospholipase D Activation by the M3 Muscarinic Receptor. J. Biol. Chem. 278: 33818-33830 [Abstract] [Full Text]  
  • Foster, D. A., Xu, L. (2003). Phospholipase D in Cell Proliferation and Cancer. Mol Cancer Res 1: 789-800 [Abstract] [Full Text]  
  • Pennock, S., Wang, Z. (2003). Stimulation of Cell Proliferation by Endosomal Epidermal Growth Factor Receptor As Revealed through Two Distinct Phases of Signaling. Mol. Cell. Biol. 23: 5803-5815 [Abstract] [Full Text]  
  • Ahn, B.-H., Kim, S. Y., Kim, E. H., Choi, K. S., Kwon, T. K., Lee, Y. H., Chang, J.-S., Kim, M.-S., Jo, Y.-H., Min, D. S. (2003). Transmodulation between Phospholipase D and c-Src Enhances Cell Proliferation. Mol. Cell. Biol. 23: 3103-3115 [Abstract] [Full Text]  
  • Koch, T., Brandenburg, L.-O., Schulz, S., Liang, Y., Klein, J., Hollt, V. (2003). ADP-ribosylation Factor-dependent Phospholipase D2 Activation Is Required for Agonist-induced {micro}-Opioid Receptor Endocytosis. J. Biol. Chem. 278: 9979-9985 [Abstract] [Full Text]  
  • Xu, L., Frankel, P., Jackson, D., Rotunda, T., Boshans, R. L., D'Souza-Schorey, C., Foster, D. A. (2003). Elevated Phospholipase D Activity in H-Ras- but Not K-Ras-Transformed Cells by the Synergistic Action of RalA and ARF6. Mol. Cell. Biol. 23: 645-654 [Abstract] [Full Text]  
  • Wang, Y., Pennock, S., Chen, X., Wang, Z. (2002). Internalization of Inactive EGF Receptor into Endosomes and the Subsequent Activation of Endosome-Associated EGF Receptors. Sci Signal 2002: pl17-pl17 [Abstract] [Full Text]  
  • Freyberg, Z., Bourgoin, S., Shields, D. (2002). Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian Cells. Mol. Biol. Cell 13: 3930-3942 [Abstract] [Full Text]  
  • Han, J. M., Kim, Y., Lee, J. S., Lee, C. S., Lee, B. D., Ohba, M., Kuroki, T., Suh, P.-G., Ryu, S. H. (2002). Localization of Phospholipase D1 to Caveolin-enriched Membrane via Palmitoylation: Implications for Epidermal Growth Factor Signaling. Mol. Biol. Cell 13: 3976-3988 [Abstract] [Full Text]  
  • Wang, Y., Pennock, S., Chen, X., Wang, Z. (2002). Endosomal Signaling of Epidermal Growth Factor Receptor Stimulates Signal Transduction Pathways Leading to Cell Survival. Mol. Cell. Biol. 22: 7279-7290 [Abstract] [Full Text]  
  • O'Luanaigh, N., Pardo, R., Fensome, A., Allen-Baume, V., Jones, D., Holt, M. R., Cockcroft, S. (2002). Continual Production of Phosphatidic Acid by Phospholipase D Is Essential for Antigen-stimulated Membrane Ruffling in Cultured Mast Cells. Mol. Biol. Cell 13: 3730-3746 [Abstract] [Full Text]  
  • Salazar, G., Gonzalez, A. (2002). Novel Mechanism for Regulation of Epidermal Growth Factor Receptor Endocytosis Revealed by Protein Kinase A Inhibition. Mol. Biol. Cell 13: 1677-1693 [Abstract] [Full Text]  
  • Skippen, A., Jones, D. H., Morgan, C. P., Li, M., Cockcroft, S. (2002). Mechanism of ADP Ribosylation Factor-stimulated Phosphatidylinositol 4,5-Bisphosphate Synthesis in HL60 Cells. J. Biol. Chem. 277: 5823-5831 [Abstract] [Full Text]  
  • Zha, X., Genest, J. Jr., McPherson, R. (2001). Endocytosis Is Enhanced in Tangier Fibroblasts. POSSIBLE ROLE OF ATP-BINDING CASSETTE PROTEIN A1 IN ENDOSOMAL VESICULAR TRANSPORT. J. Biol. Chem. 276: 39476-39483 [Abstract] [Full Text]  
  • Brymora, A., Valova, V. A., Larsen, M. R., Roufogalis, B. D., Robinson, P. J. (2001). The Brain Exocyst Complex Interacts with RalA in a GTP-dependent Manner. IDENTIFICATION OF A NOVEL MAMMALIAN Sec3 GENE AND A SECOND Sec15 GENE. J. Biol. Chem. 276: 29792-29797 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»