This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Määttä, A.
Right arrow Articles by Watt, F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Määttä, A.
Right arrow Articles by Watt, F. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2001, p. 7047-7053, Vol. 21, No. 20
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.20.7047-7053.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Gene Targeting of Envoplakin, a Cytoskeletal Linker Protein and Precursor of the Epidermal Cornified Envelope

Arto Määttä,dagger Teresa DiColandrea,Dagger Karen Groot, and Fiona M. Watt*

Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom

Received 11 May 2001/Accepted 10 July 2001

Envoplakin, a member of the plakin family of cytoskeletal linker proteins, is localized in desmosomes of stratified epithelial cells and is a component of the epidermal cornified envelope. Gene targeting in mouse embryonic stem cells was used to generate a null allele of envoplakin. No envoplakin transcripts from the targeted allele could be detected in the skin of newborn mice. Mice homozygous for the targeted allele were born in the normal Mendelian ratio and were fertile. They did not develop any discernible pathological phenotype up to the age of 1 year. The ultrastructural appearance of cornified envelopes from adult epidermis was indistinguishable between wild-type and knockout mice, and there was no evidence that the absence of envoplakin affected the subcellular distribution of periplakin or desmoplakin, two other plakins found in desmosomes. The proportion of immature cornified envelopes in the epidermis of newborn mice was greater in envoplakin-null animals than in heterozygous littermates or wild-type mice, and the envelopes had a larger surface area. This correlated with a slight delay in barrier acquisition during embryonic development. We conclude that although envoplakin is part of the scaffolding on which the cornified envelope is assembled, it is not essential for envelope formation or epidermal barrier function.

* Corresponding author. Mailing address: Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom. Phone: 44 20 7269 3528. Fax: 44 20 7269 3078. E-mail: watt{at}icrf.icnet.uk.

dagger Present address: Department of Biological Sciences, University of Durham, Durham DH1 3LE, United Kingdom.

Dagger Present address: New Link Genetics, Ames, IA 50014.

Molecular and Cellular Biology, October 2001, p. 7047-7053, Vol. 21, No. 20
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.20.7047-7053.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nachat, R., Cipolat, S., Sevilla, L. M., Chhatriwala, M., Groot, K. R., Watt, F. M. (2009). KazrinE is a desmosome-associated liprin that colocalises with acetylated microtubules. J. Cell Sci. 122: 4035-4041 [Abstract] [Full Text]  
  • Sevilla, L. M., Nachat, R., Groot, K. R., Klement, J. F., Uitto, J., Djian, P., Maatta, A., Watt, F. M. (2007). Mice deficient in involucrin, envoplakin, and periplakin have a defective epidermal barrier. JCB 179: 1599-1612 [Abstract] [Full Text]  
  • Long, H. A., Boczonadi, V., McInroy, L., Goldberg, M., Maatta, A. (2006). Periplakin-dependent re-organisation of keratin cytoskeleton and loss of collective migration in keratin-8-downregulated epithelial sheets. J. Cell Sci. 119: 5147-5159 [Abstract] [Full Text]  
  • de Guzman Strong, C., Wertz, P. W., Wang, C., Yang, F., Meltzer, P. S., Andl, T., Millar, S. E., Ho, I-C., Pai, S.-Y., Segre, J. A. (2006). Lipid defect underlies selective skin barrier impairment of an epidermal-specific deletion of Gata-3. JCB 175: 661-670 [Abstract] [Full Text]  
  • Spazierer, D., Fuchs, P., Reipert, S., Fischer, I., Schmuth, M., Lassmann, H., Wiche, G. (2006). Epiplakin Is Dispensable for Skin Barrier Function and for Integrity of Keratin Network Cytoarchitecture in Simple and Stratified Epithelia. Mol. Cell. Biol. 26: 559-568 [Abstract] [Full Text]  
  • Osada, M., Park, H. L., Nagakawa, Y., Yamashita, K., Fomenkov, A., Kim, M. S., Wu, G., Nomoto, S., Trink, B., Sidransky, D. (2005). Differential Recognition of Response Elements Determines Target Gene Specificity for p53 and p63. Mol. Cell. Biol. 25: 6077-6089 [Abstract] [Full Text]  
  • Raymond, K., Kreft, M., Janssen, H., Calafat, J., Sonnenberg, A. (2005). Keratinocytes display normal proliferation, survival and differentiation in conditional {beta}4-integrin knockout mice. J. Cell Sci. 118: 1045-1060 [Abstract] [Full Text]  
  • Jang, S.-I., Kalinin, A., Takahashi, K., Marekov, L. N., Steinert, P. M. (2005). Characterization of human epiplakin: RNAi-mediated epiplakin depletion leads to the disruption of keratin and vimentin IF networks. J. Cell Sci. 118: 781-793 [Abstract] [Full Text]  
  • Aho, S., Li, K., Ryoo, Y., McGee, C., Ishida-Yamamoto, A., Uitto, J., Klement, J. F. (2004). Periplakin Gene Targeting Reveals a Constituent of the Cornified Cell Envelope Dispensable for Normal Mouse Development. Mol. Cell. Biol. 24: 6410-6418 [Abstract] [Full Text]  
  • Kalinin, A. E., Idler, W. W., Marekov, L. N., McPhie, P., Bowers, B., Steinert, P. M., Steven, A. C. (2004). Co-assembly of Envoplakin and Periplakin into Oligomers and Ca2+-dependent Vesicle Binding: IMPLICATIONS FOR CORNIFIED CELL ENVELOPE FORMATION IN STRATIFIED SQUAMOUS EPITHELIA. J. Biol. Chem. 279: 22773-22780 [Abstract] [Full Text]  
  • Zeeuwen, P. L.J.M., van Vlijmen-Willems, I. M.J.J., Olthuis, D., Johansen, H. T., Hitomi, K., Hara-Nishimura, I., Powers, J. C., James, K. E., op den Camp, H. J., Lemmens, R., Schalkwijk, J. (2004). Evidence that unrestricted legumain activity is involved in disturbed epidermal cornification in cystatin M/E deficient mice. Hum Mol Genet 13: 1069-1079 [Abstract] [Full Text]  
  • Spazierer, D., Fuchs, P., Proll, V., Janda, L., Oehler, S., Fischer, I., Hauptmann, R., Wiche, G. (2003). Epiplakin Gene Analysis in Mouse Reveals a Single Exon Encoding a 725-kDa Protein with Expression Restricted to Epithelial Tissues. J. Biol. Chem. 278: 31657-31666 [Abstract] [Full Text]  
  • Park, G. T., Lim, S. E., Jang, S.-I., Morasso, M. I. (2002). Suprabasin, a Novel Epidermal Differentiation Marker and Potential Cornified Envelope Precursor. J. Biol. Chem. 277: 45195-45202 [Abstract] [Full Text]  
  • Zeeuwen, P. L.J.M., van Vlijmen-Willems, I. M.J.J., Hendriks, W., Merkx, G. F.M., Schalkwijk, J. (2002). A null mutation in the cystatin M/E gene of ichq mice causes juvenile lethality and defects in epidermal cornification. Hum Mol Genet 11: 2867-2875 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»