This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rébora, K.
Right arrow Articles by Daignan-Fornier, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rébora, K.
Right arrow Articles by Daignan-Fornier, B.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2001, p. 7901-7912, Vol. 21, No. 23
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.23.7901-7912.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Yeast AMP Pathway Genes Respond to Adenine through Regulated Synthesis of a Metabolic Intermediate

Karine Rébora, Christine Desmoucelles, Françoise Borne, Benoît Pinson, and Bertrand Daignan-Fornier*

Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, 33077 Bordeaux Cedex, France

Received 31 May 2001/Returned for modification 3 July 2001/Accepted 27 August 2001

In Saccharomyces cerevisiae, AMP biosynthesis genes (ADE genes) are transcriptionally activated in the absence of extracellular purines by the Bas1p and Bas2p (Pho2p) transcription factors. We now show that expression of the ADE genes is low in mutant strains affected in the first seven steps of the pathway, while it is constitutively derepressed in mutant strains affected in later steps. Combined with epistasy studies, these results show that 5'-phosphoribosyl-4-succinocarboxamide-5-aminoimidazole (SAICAR), an intermediate metabolite of the pathway, is needed for optimal activation of the ADE genes. Two-hybrid studies establish that SAICAR is required to promote interaction between Bas1p and Bas2p in vivo, while in vitro experiments suggest that the effect of SAICAR on Bas1p-Bas2p interaction could be indirect. Importantly, feedback inhibition by ATP of Ade4p, catalyzing the first step of the pathway, appears to regulate SAICAR synthesis in response to adenine availability. Consistently, both ADE4 dominant mutations and overexpression of wild-type ADE4 lead to deregulation of ADE gene expression. We conclude that efficient transcription of yeast AMP biosynthesis genes requires interaction between Bas1p and Bas2p which is promoted in the presence of a metabolic intermediate whose synthesis is controlled by feedback inhibition of Ade4p acting as the purine nucleotide sensor within the cell.

* Corresponding author. Mailing address: Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, 1, rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France. Phone: (33) 5 56 99 90 55. Fax: (33) 5 56 99 90 59. E-mail: : B.Daignan-Fornier{at}ibgc.u-bordeaux2.fr.

Molecular and Cellular Biology, December 2001, p. 7901-7912, Vol. 21, No. 23
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.23.7901-7912.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saint-Marc, C., Pinson, B., Coulpier, F., Jourdren, L., Lisova, O., Daignan-Fornier, B. (2009). Phenotypic Consequences of Purine Nucleotide Imbalance in Saccharomyces cerevisiae. Genetics 183: 529-538 [Abstract] [Full Text]  
  • Pinson, B., Vaur, S., Sagot, I., Coulpier, F., Lemoine, S., Daignan-Fornier, B. (2009). Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways. Genes Dev. 23: 1399-1407 [Abstract] [Full Text]  
  • Kowalski, D., Pendyala, L., Daignan-Fornier, B., Howell, S. B., Huang, R.-Y. (2008). Dysregulation of Purine Nucleotide Biosynthesis Pathways Modulates Cisplatin Cytotoxicity in Saccharomyces cerevisiae. Mol. Pharmacol. 74: 1092-1100 [Abstract] [Full Text]  
  • Andersen, M. P., Nelson, Z. W., Hetrick, E. D., Gottschling, D. E. (2008). A Genetic Screen for Increased Loss of Heterozygosity in Saccharomyces cerevisiae. Genetics 179: 1179-1195 [Abstract] [Full Text]  
  • Breton, A., Pinson, B., Coulpier, F., Giraud, M.-F., Dautant, A., Daignan-Fornier, B. (2008). Lethal Accumulation of Guanylic Nucleotides in Saccharomyces cerevisiae HPT1-Deregulated Mutants. Genetics 178: 815-824 [Abstract] [Full Text]  
  • Philpott, C. C., Protchenko, O. (2008). Response to Iron Deprivation in Saccharomyces cerevisiae. Eukaryot Cell 7: 20-27 [Full Text]  
  • Koehler, R. N., Rachfall, N., Rolfes, R. J. (2007). Activation of the ADE Genes Requires the Chromatin Remodeling Complexes SAGA and SWI/SNF. Eukaryot Cell 6: 1474-1485 [Abstract] [Full Text]  
  • Mateos, L., Jimenez, A., Revuelta, J. L., Santos, M. A. (2006). Purine Biosynthesis, Riboflavin Production, and Trophic-Phase Span Are Controlled by a Myb-Related Transcription Factor in the Fungus Ashbya gossypii.. Appl. Environ. Microbiol. 72: 5052-5060 [Abstract] [Full Text]  
  • Mieczkowski, P. A., Dominska, M., Buck, M. J., Gerton, J. L., Lieb, J. D., Petes, T. D. (2006). Global Analysis of the Relationship between the Binding of the Bas1p Transcription Factor and Meiosis-Specific Double-Strand DNA Breaks in Saccharomyces cerevisiae. Mol. Cell. Biol. 26: 1014-1027 [Abstract] [Full Text]  
  • Jimenez, A., Santos, M. A., Pompejus, M., Revuelta, J. L. (2005). Metabolic Engineering of the Purine Pathway for Riboflavin Production in Ashbya gossypii. Appl. Environ. Microbiol. 71: 5743-5751 [Abstract] [Full Text]  
  • Som, I., Mitsch, R. N., Urbanowski, J. L., Rolfes, R. J. (2005). DNA-Bound Bas1 Recruits Pho2 To Activate ADE Genes in Saccharomyces cerevisiae. Eukaryot Cell 4: 1725-1735 [Abstract] [Full Text]  
  • Todeschini, A.-L., Morillon, A., Springer, M., Lesage, P. (2005). Severe Adenine Starvation Activates Ty1 Transcription and Retrotransposition in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 7459-7472 [Abstract] [Full Text]  
  • Rebora, K., Laloo, B., Daignan-Fornier, B. (2005). Revisiting Purine-Histidine Cross-Pathway Regulation in Saccharomyces cerevisiae: A Central Role for a Small Molecule. Genetics 170: 61-70 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»