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Molecular and Cellular Biology, December 2001, p. 8336-8345, Vol. 21, No. 24
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.24.8336-8345.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Deficiency of Reproductive Tract alpha (1,2)Fucosylated Glycans and Normal Fertility in Mice with Targeted Deletions of the FUT1 or FUT2 alpha (1,2)Fucosyltransferase Locus

Steven E. Domino,1,* Liang Zhang,1,dagger Patrick J. Gillespie,2,Dagger Thomas L. Saunders,3 and John B. Lowe3,4

Department of Obstetrics and Gynecology,1 Department of Physiology,2 Howard Hughes Medical Institute,3 and Department of Pathology,4 The University of Michigan Medical School, Ann Arbor, Michigan 48109

Received 31 August 2001/Accepted 10 September 2001

The fucose alpha (1right-arrow2) galactose beta  structure is expressed by uterine epithelial cells in the mouse and has been implicated in blastocyst adhesion events thought to be required for murine implantation. Fucalpha (1right-arrow2)Galbeta moieties and cognate fucosyltransferases are also expressed by epithelial cells of the male reproductive tract and have been implicated in sperm maturation events that may contribute to fertilization. To determine directly if Fucalpha (1right-arrow2)Galbeta moieties are required for fertility, we have generated strains of mice that are deficient in genes encoding FUT1 and FUT2, a pair of GDP-L-fucose:beta (1right-arrow4)-D-galactosyl-R 2-alpha -L-fucosyltransferase enzymes (EC 2.4.1.69) responsible for Fucalpha (1right-arrow2)Galbeta synthesis and expression. FUT1 null mice and FUT2 null mice develop normally and exhibit no gross phenotypic abnormalities. The Fucalpha (1right-arrow2)Galbeta epitope is absent from the uterine epithelia of FUT2 null mice and from the epithelia of the epididymis of FUT1 null mice. Fully normal fertility is observed in FUT1 null intercrosses and in FUT2 null intercrosses. These observations indicate that Fucalpha (1right-arrow2)Galbeta moieties are not essential to blastocyst-uterine epithelial cell interactions required for implantation and are not required for sperm maturation events that permit fertilization and that neither the FUT loci nor their cognate fucosylated glycans are essential to normal development.

* Corresponding author. Mailing address: Department of Obstetrics and Gynecology, 6428 Medical Science Bldg. I, 1150 West Medical Center Dr., The University of Michigan, Ann Arbor, MI 48109-0617. Phone: (734) 647-9562. Fax: (734) 936-8617. E-mail: sedomino{at}med.umich.edu.

dagger Present address: Henry Ford Hospital, Detroit, MI 48202.

Dagger Present address: Pfizer Global Research & Development, Ann Arbor, MI 48105.

Molecular and Cellular Biology, December 2001, p. 8336-8345, Vol. 21, No. 24
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.24.8336-8345.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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