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Molecular and Cellular Biology, February 2001, p. 1011-1023, Vol. 21, No. 4
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.4.1011-1023.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Splicing Factor Slt11p and Its Involvement in Formation of U2/U6 Helix II in Activation of the Yeast Spliceosome

Deming Xu and James D. Friesen*

Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5G 1L6

Received 29 September 2000/Returned for modification 31 October 2000/Accepted 22 November 2000

Slt11p is a new splicing factor identified on the basis of synthetic lethality with a mutation in the 5' end of U2 snRNA, a region that is involved in intermolecular U2/U6 helix II interaction. Slt11p is required for spliceosome assembly. Our genetic results suggest that Slt11p is involved in the base-pairing interaction of U2/U6 helix II in vivo. We showed that the recombinant protein binds to RNAs with some degree of structural specificity. Slt11p also anneals RNA and binds to the resulting duplexes, which contain two separated helical regions. These RNA structures are reminiscent of U2/U6 helix II, which is formed concomitantly with U4/U6 stem II, and suggest that Slt11p facilitates the cooperative formation of helix II in association with stem II in the spliceosome. We show that Slt11p and Slu7p, a second-step factor, interact with each other both in vivo and in vitro and that the binding of Slu7p to Slt11p impairs the RNA-binding activity of the latter. These results suggest that the function of Slt11p is regulated by Slu7p in the spliceosome.

* Corresponding author. Mailing address: Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, 112 College St., Toronto, Ontario, Canada M5G 1L6. Phone: (416) 946-3016. Fax: (416) 978-8528. E-mail: james.friesen{at}utoronto.ca.

Molecular and Cellular Biology, February 2001, p. 1011-1023, Vol. 21, No. 4
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.4.1011-1023.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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