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Molecular and Cellular Biology, February 2001, p. 1272-1284, Vol. 21, No. 4
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.4.1272-1284.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Candida albicans INT1-Induced Filamentation in Saccharomyces cerevisiae Depends on Sla2p

Catherine M. Asleson,1,dagger Eric S. Bensen,1 Cheryl A. Gale,2 A.-S. Melms,3 Cornelia Kurischko,3 and Judith Berman1,4,*

Department of Genetics, Cell Biology, and Development1 and Department of Microbiology,4 University of Minnesota, St. Paul, Minnesota 55108; Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota 554552; and Department of Cell and Molecular Biology, Hans-Knöll-Institut für Naturstoff-Forschung e.V., D-07745 Jena, Germany3

Received 19 September 2000/Returned for modification 17 October 2000/Accepted 17 November 2000

The Candida albicans INT1 gene is important for hyphal morphogenesis, adherence, and virulence (C. Gale, C. Bendel, M. McClellan, M. Hauser, J. M. Becker, J. Berman, and M. Hostetter, Science 279:1355-1358, 1998). The ability to switch between yeast and hyphal morphologies is an important virulence factor in this fungal pathogen. When INT1 is expressed in Saccharomyces cerevisiae, cells grow with a filamentous morphology that we exploited to gain insights into how C. albicans regulates hyphal growth. In S. cerevisiae, INT1-induced filamentous growth was affected by a small subset of actin mutations and a limited set of actin-interacting proteins including Sla2p, an S. cerevisiae protein with similarity in its C terminus to mouse talin. Interestingly, while SLA2 was required for INT1-induced filamentous growth, it was not required for polarized growth in response to several other conditions, suggesting that Sla2p is not required for polarized growth per se. The morphogenesis checkpoint, mediated by Swe1p, contributes to INT1-induced filamentous growth; however, epistasis analysis suggests that Sla2p and Swe1p contribute to INT1-induced filamentous growth through independent pathways. The C. albicans SLA2 homolog (CaSLA2) complements S. cerevisiae sla2Delta mutants for growth at 37°C and INT1-induced filamentous growth. Furthermore, in a C. albicans Casla2/Casla2 strain, hyphal growth did not occur in response to either nutrient deprivation or to potent stimuli, such as mammalian serum. Thus, through analysis of INT1-induced filamentous growth in S. cerevisiae, we have identified a C. albicans gene, SLA2, that is required for hyphal growth in C. albicans.

* Corresponding author. Mailing address: Department of Genetics, Cell Biology, and Development, University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN 55108. Phone: (612) 625-1971. Fax: (612) 625-5754. E-mail: judith{at}cbs.umn.edu.

dagger Present address: Cargill Dow LLC, Minnetonka, MN 55345.

Molecular and Cellular Biology, February 2001, p. 1272-1284, Vol. 21, No. 4
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.4.1272-1284.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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